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Registros recuperados: 7
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Heterologous expression, purification and refolding of an anti-listerial peptide produced by Pediococcus acidilactici K7 Electron. J. Biotechnol.
Halami,Prakash M; Chandrashekar,Arun.
The fusion protein, 6XHis-Xpress-PedA was constructed and expressed in Escherichia coli BL21 (DE3). The presence of a 12.8 kDa recombinant protein, localized in inclusion bodies (IBs) at high concentration, was confirmed by SDS-PAGE analysis and by western blotting using anti-His antibody. The rec-pediocin was purified by Nickel-nitrilotriacetic acid beads and refolded using 5 mM of β-mercaptoethanol along with 1 M glycine. Results indicated that the refolded rec-pediocin had an early elution profile in the RP-HPLC when compared to the unfolded protein and it exhibited biological activity against Listeria monocytogenes V7 which was approximately 25 times less active compared to native counterpart. The final yield of purified rec-pediocin was 3...
Tipo: Journal article Palavras-chave: Fusion protein; Inclusion bodies; In vitro refolding; Pediocin PA-1; Pediococcus acidilactici; RP-HPLC.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400009
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Reversed phase HPLC determination of zidovudine in rat plasma and its pharmacokinetics after a single intranasal dose administration Biol. Res.
MAINARDES,RUBIANA M; PALMIRA D. GREMIÃO,MARIA.
The development and validation of a simple and accurate method based on HPLC with ultraviolet detection for the quantification of zidovudine in rat plasma and its application to a pharmacokinetic study following a single intranasal dose zidovudine is described. Zidovudine was extracted from the plasma using a single-step deproteinization. Chromatographic separation of zidovudine from interfering components was achieved with a C-18 reverse phase column, a mobile phase consisting of a mixture of sodium acetate buffer (55mM) with pH adjusted to 7.0 and acetonitrile (91:9 v/v) and UV detection set at 265 nm. The method was linear from 100 to 10000 ng.mL"¹ (r² > 0.9995), and zidovudine had a mean recovery from plasma of 92.8%. The coefficient of variation of...
Tipo: Journal article Palavras-chave: RP-HPLC; Validation; AZT; Pharmacokinetics; Intranasal delivery.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300010
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Padronização de método cromatográfico para caracterização do queijo coalho. Repositório Alice
FONTENELE, M. A.; VASCONCELOS, A. S. do E.; FOLSTA, K. C. B. M.; BASTOS, M. do S. R..
2010
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Queijo coalho; Cromatografia; Padronização; RP-HPLC.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/handle/doc/883175
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Quality evaluation of simvastatin compounded capsules BJPS
Marques-Marinho,Flávia Dias; Zanon,José Carlos da Costa; Sakurai,Emilia; Reis,Ilka Afonso; Lima,Angélica Alves; Vianna-Soares,Cristina Duarte.
Simvastatin is commercially available as tablets and compounded capsules in Brazil. Very few reports regarding these capsules' quality, and consequently their efficacy, are available. The pharmaceutical quality of 30 batches of 20 mg simvastatin capsules from the market was evaluated by weight determination, content uniformity, disintegration (Brazilian Pharmacopeia), assay and dissolution test (USP32 tablet monograph). A HPLC method was developed for assay, content uniformity and dissolution test, and specifications were also established. Out of the 30 batches evaluated, 29 showed capsule disintegration within 45 min and individual weight variation was within ±10% or ±7.5% relative to average weight, for < or &gt; 300 mg, respectively. Only 27...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Compounded simvastatin capsules; Quality control; RP-HPLC.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300007
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Simultaneous RP-HPLC determination of sparfloxacin and dexamethasone in pharmaceutical formulations BJPS
Razzaq,Syed Naeem; Ashfaq,Muhammad; Mariam,Irfana; Khan,Islam Ullah; Razzaq,Syed Saleem.
The present study describes the development and subsequent validation of simple and accurate stability indicating RP-HPLC method for the determination of sparfloxacin and dexamethasone in pharmaceutical formulations in the presence of their stress-induced degradation products. Both the drugs and their stress-induced degradation products were separated within 10 minutes using C8 column and mixture of methanol and 0.02 M phosphate buffer pH 3.0 (60:40 v/v, respectively) as mobile phase at 270 nm using diode array detector. Regression analysis showed linearity in the range of 15-105 µg/mL for sparfloxacin and 5-35 µg/mL for dexamethasone. All the analytes were adequately resolved with acceptable tailing. Peak purity of the two drugs was also greater than...
Tipo: Info:eu-repo/semantics/article Palavras-chave: RP-HPLC; Sparfloxacin; Dexamethasone; Degradation products; Stability studies.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200012
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Stability indicating RP-HPLC method development and validation of cefepime and amikacin in pure and pharmaceutical dosage forms BJPS
Kalyani,Lella; Rao,Chava Venkata Nageswara.
A simple, accurate, isocratic stability indicating RP-HPLC method was developed for the determination of cefepime and amikacin in Pure and its pharmaceutical formulations. The method consists of methanol: acetonitrile:acetate buffer 75:20:05 (v/v) mobile phase at pH 5.1 with C18 column as stationary phase. The flow rate and detection wave length were 1.0 mL/min and 212 nm respectively. The linearity range for the method was found to be 2.5-25 µg/mL for amikacin and 10-100 µg/mL cefepime respectively. The developed method was validated as per ICH guidelines and the results of all the validation parameters were well within their acceptance values. Also the forced degradation studies were conducted with standard drugs. Degradation products formed during the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cefepime/method development and validation; Amikacin/method development and validation; RP-HPLC.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300607
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Método de Análise de Tocoferóis em Azeite de Oliva por Cromatografia Líquida de Alta Eficiência em Fase Reversa. Infoteca-e
MACHADO, A. F. de F.; ANTONIASSI, R.; GUEDES, A. M. M.; WILHELM, A. E..
Os tococromanóis são normalmente analisados por cromatografia líquida de alta eficiência (HPLC) em fase normal, conforme indicado pelos métodos oficiais. Considerando que a cromatografia em fase reversa (RP) apresenta algumas vantagens em relação à fase normal, neste trabalho um método baseado em RP-HPLC foi adaptado e validado para análise de azeites de oliva brasileiros. Para garantir a seletividade e a sensibilidade do método, foi selecionado o detector de fluorescência. O método apresentou boa linearidade (R2?0,995), com limites de detecção e quantificação de 2,5?0,7 ng e 7,4?2,1 ng de ?-tocoferol, respectivamente. O teor de tocoferóis totais nas amostras de azeite analisadas foi de 146-385 mg/kg, sendo ?-tocoferol o principal componente (78-96 %). A...
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: HPLC-FLR; RP-HPLC; Azeites brasileiros; Tecnologia de Alimento; Vitamina E; Azeite.
Ano: 2018 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1104313
Registros recuperados: 7
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