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Registros recuperados: 11
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Development of an indirect ELISA based on recombinant capsid protein to detect antibodies to bovine leukemia virus BJM
Andreolla,Ana Paula; Erpen,Luana Marina Scheer; Frandoloso,Rafael; Kreutz,Luiz Carlos.
Abstract Serological testing and culling infected animals are key management practices aiming eradication of bovine leukemia virus infection. Here, we report the development of an indirect ELISA based on BLV recombinant capsid protein (BLVp24r) to detect anti-BLV antibodies in cattle serum. The BLVp24r was expressed in Escherichia coli and purified by affinity chromatography, and then used to set up the ELISA parameters. The Polysorp ® plate coated with 50 ng of antigen/well and bovine serum diluted 1:100 gave the best results during standardization. Using sera from infected and non-infected cattle we set up the cutoff point at 0.320 (OD450 nm) with a sensitivity of 98.5% and specificity of 100.0%. Then, we tested 1.187 serum samples from dairy (736...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine leukemia virus; Retrovirus; Diagnosis; ELISA.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500068
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Detección de anticuerpos contra lentivirus de pequeños rumiantes en fetos ovinos y caprinos Veterinaria México
Arcila López,Gerardo; Martínez Rodríguez,Humberto Alejandro; Tórtora Pérez,Jorge.
Se evaluó la presencia de anticuerpos contra los virus del Maedi-Visna (MV) y la artritis encefalitis caprina (AEC) en fetos ovinos y caprinos obtenidos de hembras gestantes sacrificadas para consumo en México. Se recolectaron sueros de 54 fetos caprinos y 65 fetos ovinos que se evaluaron mediante ELISA indirecto. Se detectaron anticuerpos en cinco fetos caprinos de 80 días de gestación y en cuatro fetos ovinos de 90 a 100 días; tres fetos caprinos y dos ovinos de 60 a 65 días de gestación resultaron sospechosos. Se confirma la posibilidad de la infección uterina por estos retrovirus y se discuten sus consecuencias en las actuales estrategias de control y erradicación de las enfermedades.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Retrovirus; Ovinos; Caprinos; Pequeños rumiantes; Artritis encefalitis caprina; Maedi-Visna; Inmunidad fetal; Infección transplacentaria; Infección uterina.
Ano: 2012 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0301-50922012000100002
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Viral Research in Brazilian Owls (Tyto alba and Rhinoptynx clamator) by Transmission Electron Microscopy International Journal of Morphology
Catroxo,M. H. B; Taniguchi,D. L; Melo,N. A; Milanelo,L; Petrella,S; Alves,M; Martins,A. M. C. R. P. F; Rebouças,M. M.
The barn-owl (Tyto Alba) and striped-owl (Rhinoptynx clamator) belong respectively to the families Tytonidae and Strigidae. Avian paramyxoviruses have been isolated from a variety of species of wild and domestic birds wordlwide causing diverse clinical symptoms and signs. Paramyxoviruses belong to the family Paramyxoviridae and Avulovirus genus, including nine serotypes (APMV 1 to 9). The lymphoid leukosis is a retrovirus-induced neoplasia. The avian retroviruses belong to the Retroviridae family and to the Alpharetrovirus genus. Coronaviruses can cause respiratory and enteric disease in several species of birds. They belong to the Coronaviridae family and to the groups 3a e 3c. In this study, we describe the presence of viruses in four owls, two barn owls...
Tipo: Journal article Palavras-chave: Paramyxovirus; Coronavirus; Retrovirus; Owls; Transmission electron microscopy.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200047
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Ocorrência do vírus da leucemia felina em Felis cattus em Belo Horizonte Arq. Bras. Med. Vet. Zootec.
Coelho,F.M.; Maia,M.Q.; Luppi,M.M.; Costa,E.A.; Luiz,A.P.M.F.; Ribeiro,N.A.; Bomfim,M.R.Q.; Fonseca,F.G.; Resende,M..
Blood samples from 1,072 domestic cats of nine administrative regions of Belo Horizonte, MG, were collected and tested using PCR nested for the occurrence of feline leukemia virus (FeLV). Overall occurrence was 47.5% (507/1072) being North (68.1%) and East (54.4%) the most prevalent areas. Epidemiological data showed that FeLV infection was very common among examined cats and breed neither gender nor were predisposing factors for FeLV. The results suggest that the agglomeration of a large number of cats in the same environment can be an important factor for the increase in the rate of transmission of this retrovirus among domestic cats in the studied city.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cat; Feline; Leukemia; Retrovirus; PCR-nested.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300037
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Autoimmunity and molecular mimicry in tropical spastic paraparesis/human T-lymphotropic virus-associated myelopathy BJMBR
García-Vallejo,F.; Domínguez,M.C.; Tamayo,O..
Viruses share antigenic sites with normal host cell components, a phenomenon known as molecular mimicry. It has long been suggested that viral infections might trigger an autoimmune response by several mechanisms including molecular mimicry. More than 600 antiviral monoclonal antibodies generated against 11 different viruses have been reported to react with 3.5% of cells specific for uninfected mouse organs. The main pathological feature of tropical spastic paraparesis/human T-lymphotropic virus type I (HTLV-I)-associated myelopathy (TSP/HAM) is a chronic inflammation of the spinal cord characterized by perivascular cuffing of mononuclear cells accompanied by parenchymal lymphocytic infiltration. We detected the presence of autoantibodies against a 98- to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HTLV-I; TSP/HAM; Retrovirus; Molecular mimicry.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200013
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Retroviral-mediated overexpression of human bone morphogenetic protein 2 affects human mesenchymal stem cells during monolayer proliferation: A cautionary note Electron. J. Biotechnol.
Neumann,Alexander J; Alini,Mauro; Archer,Charles W; Stoddart,Martin J.
Background: Retroviral vectors are commonly used for gene transfer applications and they represent an effective way to provide a sustained delivery of a bioactive factor in basic research and tissue engineering applications. Cells that have been transduced with retroviral vectors ex vivo are usually amplified on tissue culture plastic, for a prolonged period of time, in order to obtain sufficient cell numbers prior to the experiment of interest. However, the effect of the transgene product on the transduced cells, during this period of time, is rarely, if ever, investigated. The current study investigated if transduction with a VSG.G pseudotyped retroviral vector expressing human bone morphogenetic protein 2 (Rv.BMP-2) influences the gene expression...
Tipo: Journal article Palavras-chave: Constitutive expression; Controls; Gene transfer; Monolayer expansion; Retrovirus.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000200007
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Etude d'une prolifération hémocytaire chez la moule Mytilus edulis ArchiMer
Noel, Danièle.
Hemic proliferative disease in the bay mussel, Mytilus edulis, has been studied following mortalities in Washington State (USA). The disease has been reproduced in the labortory. The establishment of this experimental model led to study tumoral characteristics of the atypic hemocytes : ultrastrure, in vitro cultivation, transplantation. The infectious etiology of this tumor has been verified. It has been hypothesized to result from a viral infection since retrovirus-like particules have been observed following induction by bromodeoxyuridine. Specific monoclonal antibodies against atypic hemocytes have been obtained and characcterized
Tipo: Text Palavras-chave: Mytilus; Mollusque; Hémocyte; Tumeur; Ulstrastructure; In vitro culture; Transplantation; Rétrovirus; Anticorps monoclonaux; Mytilus; Mollusca; Hemocyte; Tumor; Ultrastructure; In vitro cultivation; Transplantation; Retrovirus; Monoclonal antibodies.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/00428/53930/55057.pdf
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Beyond retrovirus infection: HIV meets gene therapy Genet. Mol. Biol.
Silva,Flávia Helena da; Dalberto,Tiago Pires; Nardi,Nance Beyer.
The human immunodeficiency virus (HIV) is classified as a retrovirus because of its RNA genome and the fact that it requires reverse transcriptase to convert it into DNA. This virus belongs to the lentivirinae subfamily and is able to infect quiescent cells but is better known for its association with acquired immunodeficiency syndrome (AIDS) and can be described as one of the most effective vectors for gene transfer. Biosafety concerns are present whenever viral vectors are employed but are particularly pertinent to the development of HIV-based vectors. Insertional mutagenesis and the production of new replication-competent viruses (RCV) have been pointed to as major problems, but experimental data have shown that safe protocols can be developed for their...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HIV; MLV; Retrovirus; Lentivirus; Gene therapy.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200027
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Combating oncogene activation associated with retrovirus-mediated gene therapy of X-linked severe combined immunodeficiency BJMBR
Strauss,B.E.; Costanzi-Strauss,E..
A successful gene therapy clinical trial that also encountered serious adverse effects has sparked extensive study and debate about the future directions for retrovirus-mediated interventions. Treatment of X-linked severe combined immunodeficiency with an oncoretrovirus harboring a normal copy of the gc gene was applied in two clinical trials, essentially curing 13 of 16 infants, restoring a normal immune system without the need for additional immune-related therapies. Approximately 3 years after their gene therapy, tragically, 3 of these children, all from the same trial, developed leukemia as a result of this experimental treatment. The current understanding of the mechanism behind this leukemogenesis involves three critical and cooperating factors,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Retrovirus; Insertional mutagenesis; Severe combined immune deficiency; Gene therapy; Adverse event; Clinical trial.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500002
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Efficient retrovirus-mediated transfer of cell-cycle control genes to transformed cells BJMBR
Strauss,B.E.; Costanzi-Strauss,E..
The use of gene therapy continues to be a promising, yet elusive, alternative for the treatment of cancer. The origins of cancer must be well understood so that the therapeutic gene can be chosen with the highest chance of successful tumor regression. The gene delivery system must be tailored for optimum transfer of the therapeutic gene to the target tissue. In order to accomplish this, we study models of G1 cell-cycle control in both normal and transformed cells in order to understand the reasons for uncontrolled cellular proliferation. We then use this information to choose the gene to be delivered to the cells. We have chosen to study p16, p21, p53 and pRb gene transfer using the pCL-retrovirus. Described here are some general concepts and specific...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene therapy; Clinical trial; Retrovirus; Animal model; Cell cycle; Growth arrest.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700016
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Characterization of the long-terminal repeat single-strand tail-binding site of Moloney-MuLV integrase by crosslinking Biol. Res.
VERA,JORGE; VALENZUELA,BEATRIZ; ROTH,MÓNICA J; LEÓN,ÓSCAR.
Processing of viral DNA by retroviral integrase leaves a dinucleotide single-strand overhang in the unprocessed strand. Previous studies have stressed the importance of the 5' single-stranded (ss) tail in the integration process. To characterize the ss-tail binding site on M-MuLV integrase, we carried out crosslinking studies utilizing a disintegration substrate that mimics the covalent intermediate formed during integration. This substrate carried reactive groups at the 5' ss tail. A bromoacetyl derivative with a side chain of 6 A was crosslinked to the mutant IN 106-404, which lacks the N-terminal domain, yielding a crosslinked complex of 50 kDa. Treatment of IN 106-404 with N-ethylmaleimide (NEM) prevented crosslinking, suggesting that Cys209 was...
Tipo: Journal article Palavras-chave: Integrase; Retrovirus; Crosslinking.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100009
Registros recuperados: 11
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