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| Mello,S.L.V.; Henry,M.; Souza,M.C.; Oliveira,S.M.P.. |
| The aim of this study was to evaluate the longevity of donkey sperm comparing the rich seminal fraction and the whole semen in two extenders, Kenney and modified Baken extenders. Semen of five donkeys were collected through an open-end artificial vagina once a week for five consecutive weeks. The two first jets (rich fraction) of semen were collected separately from the rest of the ejaculate. Whole semen samples were obtained mixing proportionally part of the rich with part of the poor seminal fractions. Seminal samples were immediately diluted 1:1 in each extender and maintained at room temperature during sperm concentration analysis. Samples were further diluted to rich 50×10(6) sperm per ml, cooled in a refrigerator at the initial rate of -0.6° C/min... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Donkey; Cooled semen; Sperm longevity. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400015 |
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| OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G.; PENEIRAS, A. B. V.; BALARO, M. F. A.; FREITAS, V. J. F.; FONSECA, J. F. da; BRANDÃO, F. Z.. |
| Abstract: Sperm capacitation is an essential event for fertilization; however, it decreases the sperm lifespan and viability. The aim of this study was to evaluate the effects of four sperm selection techniques on sperm capacitation and viability after incubation. A pool of frozen-thawed sperm from 10 Santa Inês rams was used. The samples were submitted to one of the following sperm selection techniques: sperm washing, Percoll gradient, mini-Percoll gradient, Swim-up and control group. At mini-Percoll technique, was used 400 microliters of 90% and 45% gradients and a centrifugation at 500 xg for 5 minutes. In Percoll, was used 1 mL of each gradient and a centrifugation at 700 xg for 10 minutos. During Swim-up, the sperm was incubated in 1 ml of SPERM-TALP... |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: Longevidade espermática; Seleção espermática; Raça Santa Inês; Sperm longevity; Sperm selection; Embryo Technology.; Ovino; Carneiro; Reprodução animal; Transferência de Embrião; Sêmen; Espermatozóide; Sheep; Reproduction; Rams.. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1074502 |
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