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CaV 3.1 and CaV 3.3 account for T-type Ca2+ current in GH3 cells BJMBR
Mudado,M.A.; Rodrigues,A.L.; Prado,V.F.; Beirão,P.S.L.; Cruz,J.S..
T-type Ca2+ channels are important for cell signaling by a variety of cells. We report here the electrophysiological and molecular characteristics of the whole-cell Ca2+ current in GH3 clonal pituitary cells. The current inactivation at 0 mV was described by a single exponential function with a time constant of 18.32 ± 1.87 ms (N = 16). The I-V relationship measured with Ca2+ as a charge carrier was shifted to the left when we applied a conditioning pre-pulse of up to -120 mV, indicating that a low voltage-activated current may be present in GH3 cells. Transient currents were first activated at -50 mV and peaked around -20 mV. The half-maximal voltage activation and the slope factors for the two conditions are -35.02 ± 2.4 and 6.7 ± 0.3 mV (pre-pulse of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Calcium channel; T-type; Electrophysiology; RT-PCR; GH3 cells.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600020
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