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The establishment of in?vitro culture and drug screening systems for a newly isolated strain of Trypanosoma equiperdum OAK
Suganuma, Keisuke; Yamasaki, Shino; Molefe, Nthatisi Innocentia; Musinguzi, Peter Simon; Davaasuren, Batdorj; Mossaad, Ehab; Narantsatsral, Sandagdorj; Battur, Banzragch; Battsetseg, Badgar; Inoue, Noboru.
Dourine is caused by Trypanosoma equiperdum via coitus with an infected horse. Although dourine is distributed in Equidae worldwide and is listed as an internationally important animal disease by the World Organization for Animal Health (OIE), no effective treatment strategies have been established. In addition, there are no reports on drug discovery, because no drug screening system exists for this parasite. A new T.?equiperdum strain was recently isolated from the genital organ of a stallion that showed typical symptoms of dourine. In the present study, we adapted T.?equiperdum IVM-t1 from soft agarose media to HMI-9 liquid media to develop a drug screening assay for T.?equiperdum. An intracellular ATP-based luciferase assay using CellTiter-Glo reagent...
Palavras-chave: Colorimetric assay; Drug screening system; Liquid culture; Luciferase assay; Trypanosoma equiperdum.
Ano: 2017 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4538
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Isolation, cultivation and molecular characterization of a new Trypanosoma equiperdum strain in Mongolia OAK
Suganuma, Keisuke; Narantsatsral, Sandagdorj; Battur, Banzragch; Yamasaki, Shino; Otgonsuren, Davaajav; Musinguzi, Simon Peter; Davaasuren, Batdorj; Battsetseg, Badgar; Inoue, Noboru.
Background: Trypanosoma equiperdum causes dourine via sexual transmission in Equidae. T. equiperdum is classified under the subgenus Trypanozoon along with the T. brucei sspp. and T. evansi; however, the species classification of Trypanozoon remains a controversial topic due to the limited number of T. equiperdum reference strains. In addition, it is possible that some were misclassified T. evansi strains. Thus, there is a strong need for a new T. equiperdum strain directly isolated from the genital mucosa of a horse with a clinically-and parasitologically-confirmed dourine infection. Methods: Trypanosomes isolated from the urethral tract of a stallion with suspected dourine, were directly cultivated using soft agarose media at 37 degrees C in 5 % CO2. For...
Palavras-chave: Dourine; In vitro culture; Maxicircle DNA; Mongolia; Soft agarose media; Trypanosoma equiperdum.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4442
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