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Expression analysis of vasa in Asian paddle crab (Charybdis japónica) exposed to Bisphenol A
Provedor de dados: 69
Autores: Chen,Jianhua; Wang,Cuihua; Gao,Huan; Yan,Binlun.
Background: Bisphenol A (BPA) is an endocrine-disrupting chemical (EDC) with a weak estrogen-like activity in fish that is found ubiquitously in aquatic environments. However, there has been little study about BPA on the endocrine disrupting effects of crab. In the present study, cDNA of vasa was cloned and characterized in the Charybdis japonica. Histological structures of testis and expression patterns of vasa gene in the testis of C. japonica after treatment with BPA were investigated. Results: The cDNA of vasa is composed of 3051 bp with a 2166 bp open reading frame encoding 721 AA. The deduced amino acid sequence contained eight conserved domains of the DEAD-box protein family. The tissue distribution showed that vasa mRNA was specifically expressed...
Tipo: Journal article
Palavras-chave: Aquatic environments; BPA; DEAD-box; Endocrine disrupting; Expression analysis; Expression patterns; Histological observation; Vasa; Vasa cDNA; Vasa gene; Vasa mRNA.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600008