Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 10
Primeira ... 1 ... Última
Imagem não selecionada

Imprime registro no formato completo
Variation in growth at different temperatures and production and size of conidia in hybrid strains of Verticillium lecanii (Lecanicillium spp.) (Deuteromycotina: Hyphomycetes) OAK
AIUCHI, Daigo; BABA, Yukiko; INAMI, Keigo; SHINYA, Ryoji; TANI, Masayuki; KOIKE, Masanori; 相内, 大吾; 谷, 昌幸; 小池, 正徳.
In a previous study, we confirmed alterations in several fungal traits in hybrid strains of Verticillium lecanii generated via protoplast fusion. In the present study, we surveyed radial colony growth in different temperature regimes as well as conidia productivity and conidia size of hybrid strains of V. lecanii to elucidate the various primary fungal properties of these strains and to determine the correlation between these properties and virulence to insect pests. Minimum temperature thresholds for all tested strains (except Vertalec®) were below 5°C. Optimal temperatures for colony growth were 20–25°C. Colony growth at high temperature was good or poor depending on the genotype. Temperature and strain significantly affected colony growth. Conidial...
Palavras-chave: Lecanicillium spp; Phenotypic variation; Protoplast fusion; Temperature growth response; Verticillium lecanii.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2848
Imagem não selecionada

Imprime registro no formato completo
Bacillus subtilis for the control of powdery mildew on cucumber and zucchini squash. Repositório Alice
BETTIOL, W.; GARIBALDI, A.; MIGHELI, Q..
Application of concentrated metabolites of Bacillus subtilis - CMBS - (5,000 ug/mL) one and 24 h before or after inoculation of Sphaerotheca fuliginea (3 x 10 4 conidia/mL) reduced the number of lesions on cucumber leaves by 90-99%. The average number of lesions on control plants was 16.7 per leaf. A wettable powder product formulated with cells (10%) and metabolites (10%) of B. subtilis (WPBS), and CMBS sprayed on cucumber plants (1,000 ug/mL and 10,000 ug/mL) twice a week totally controlled powdery mildew. In the control treatment, 18 days after the first spray, the percent leaf surface covered by lesions was 99.0 and 46.7%, on the cotyledonary and expanded leaves, respectively. In the control treatment, 30 days after the first spray, the percent leaf...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Biological control; Resistance; Control methods; Fungicides; Natural enemies; Tilletiopsis spp; Ampelomyces quisqualis; Cladosporium sp; Verticillium lecanii; Acremonium alternatum; Controle biológico; Resistência; Métodos de controle; Fungicidas; Inimigos naturais.
Ano: 1997 URL: http://www.alice.cnptia.embrapa.br/handle/doc/12884
Imagem não selecionada

Imprime registro no formato completo
Efeito de diferentes meios de cultura e da luz no crescimento e na esporulação de Verticillium lecanii. Repositório Alice
BIZI, R. M.; GRIGOLETTI JÚNIOR, A.; AUER, C. G.; LEITE, M. S. P..
2003
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Verticillium lecanii; Fungo; Entomopatógeno; Controle biológico; Arroz.
Ano: 2003 URL: http://www.alice.cnptia.embrapa.br/handle/doc/308549
Imagem não selecionada

Imprime registro no formato completo
Pre-inoculation of cucumber roots with Verticillium lecanii (Lecanicillium muscarium) induces resistance to powdery mildew OAK
Hirano, Emiko; Koike, Masanori; Aiuchi, Daigo; Tani, Masayuki; 平野, 恵美子; 小池, 正徳; 相内, 大吾; 谷, 昌幸.
The ability of Verticillium lecanii (Lecanicillium muscarium; B-2) to induce host resistance and defense responses against subsequent challenge with cucumber powdery mildews was examined in a glasshouse. A root test showed that, after inoculation with V. lecanii blastspore on cucumber roots, induction of systemic resistance in those V. lecanii pre-inoculated plants engendered significantly fewer lesions and reduced disease severity compared with non-inoculated plants. Furthermore, in plants inoculated with V. lecanii blastspore on their roots, the fungi showed high colonizing ability on cucumber rhizoplane and inside root tissues. Nevertheless, activities of peroxidase (PO) and phenylalanine ammonia-lyase (PAL) increased slightly, but not significantly. In...
Palavras-chave: Induced resistance; Lecanicillium muscarium; Powdery Mildew; Verticillium lecanii.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2277
Imagem não selecionada

Imprime registro no formato completo
Efeito de diferentes regimes de luminosidade na produção de Verticillium lecanii (Zimm.) Viégas em meio sólido. Repositório Alice
RIBEIRO, R. D.; CAMARGO, J. M. M.; ZALESKI, S. R. M.; LEITE, M. S. P.; PENTEADO, S. do R. C.; GRIGOLETTI JÚNIOR, A..
2003
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Fungo; Verticillium lecanii; Produção; Luminosidade.
Ano: 2003 URL: http://www.alice.cnptia.embrapa.br/handle/doc/308579
Imagem não selecionada

Imprime registro no formato completo
Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii BJM
Zhu,Yanping; Pan,Jieru; Qiu,Junzhi; Guan,Xiong.
Entomopathogenic fungus Verticillium lecanii is a promising whitefly and aphid control agent. Chitinases secreted by this insect pathogen have considerable importance in the biological control of some insect pests. An endochitinase gene Vlchit1 from the fungus was cloned and overexpressed in Escherichia coli. The Vlchit1 gene not only contains an open reading frame (ORF) which encodes a protein of 423 amino acids (aa), but also is interrupted by three short introns. A homology modelling of Vlchit1 protein showed that the chitinase Vlchit1 has a (α/β)8 TIM barrel structure. Overexpression test and Enzymatic activity assay indicated that the Vlchit1 is a functional enzyme that can hydrolyze the chitin substrate, so the Vlchit1 gene can service as a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Cloning; Overexpression; Homology modeling; Verticillium lecanii.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200022
Imagem não selecionada

Imprime registro no formato completo
予防型微生物農薬 OAK
小池, 正徳; 杉本, みどり; 東尾, 俊基.
Palavras-chave: 微生物農薬; Verticillium lecanii.
Ano: 2003 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/842
Imagem não selecionada

Imprime registro no formato completo
Reclassification of Japanese Isolates of Verticillium lecanii to Lecanicillium spp OAK
小池, 正徳; 杉本, みどり; 相内, 大吾; 長尾, 英幸; 新谷, 良治; 谷, 昌幸; 倉持, 勝久.
Recently, Verticillium lecanii was transferred and subdivided into mainly Lecanicillium lecanii, L. muscarium, and L. longisporum according to morphological observations and PCR-RFLPs of ITS, mtDNA and β-tubulin. To clarify whether 65 isolates of V. lecanii of mainly Japanese isolates were identified as Lecanicillium spp., conidia size and the formation of secondary phialide formation were investigated under a light microscope, and DNA polymorphism in the j8-tubulin gene of V. lecanii was analyzed by PCR-RFLP. These results indicated that the examined isolates belonged to L. muscarium (60 isolates) and L. longisporum (4 isolates), except for one isolate which alone did not belong to both Lecanicillium spp.
Palavras-chave: Conidia size; Lecanicillium spp.; Secondaryphialide formation; Verticillium lecanii.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2534
Imagem não selecionada

Imprime registro no formato completo
昆虫寄生菌Verticillium lecanii (Lecanicillium spp.) によるダイコンバーティシリウム黒点病の生物防除 OAK
楠木, 芳; 川井, 彩子; 相内, 大吾; 小池, 正徳; 谷, 昌幸; 倉持, 勝久; Kusunoki, Kaori; Kawai, Ayako; Aiuchi, Daigo; Koike, Masanori; Tani, Masayuki; Kuramochi, Katsuhisa.
ダイコンバーティシリウム黒点病に対する防除効果を昆虫寄生菌Verticillium lecaniiを用いて検討した。温室試験と圃場試験でV. lecaniiの7菌株(A-2、B-2、C-1、2aF30、2aF31、2aF43、AaF101)を用いて実施した。V. lecaniiの分生胞子を処理したポット試験では全てのV. lecanii菌株で高い防除効果があり、病原菌単独処理と比較して有意な差が認められた。なかでもB-2が最も高い防除効果を示し、病原菌単独処理の発病率51.2%と比較して31.4%となった。V. lecaniiのフスマ培養土を用いたポット試験における病徴抑制効果は病原菌単独処理区との有意な差が認められたのはC-1処理区のみであった。A-2、B-2処理区においても抑制効果の傾向がみられたが、生長促進効果では顕著な差はみられなかった。また、圃場試験では全てのV. lecanii処理区で発病が抑制された。特にB-2、C-1においては高い発病抑制効果があり、病原菌単独処理区の半分以下に抑制していた。商品価値調査においてはV. lecanii処理区で商品価値のあるダイコンの数が優った。以上の結果から、病原菌単独処理と有意な差が認められなかったものもあるが、V. lecaniiはダイコンバーティシリウム黒点病を抑制するのに有効である傾向が確認された。よって、V. lecaniiはダイコンバーティシリウム黒点病に対して生物防除資材として有望であることが示唆された。 Seven strains of Verticillium lecanii were tested for their capacity to control Verticillium black-spot of Japanese radish...
Palavras-chave: ダイコンバーティシリウム黒点病; Black spot of Japanese radish; Verticillium dahliae; Verticillium lecanii.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/96
Imagem não selecionada

Imprime registro no formato completo
Screening of Verticillium lecanii (Lecanicillium spp.) Hybrid Strains Based on Evaluation of Pathogenicity against Cotton Aphid and Greenhouse Whitefly, and Viability on the Leaf Surface OAK
相内, 大吾; 馬場, ゆき子; 稲見, 圭吾; 新屋, 良治; 谷, 昌幸; 倉持, 勝久; 堀江, 早弥佳; 小池, 正徳.
In a previous study, protoplast fusion was performed among 3 strains of the entomopathogenic fungi Verticillium lecanii, as a result of which 174 hybrid strains were obtained. The aim of the present study was to screen desirable hybrid strains that have a wide host range or durable effects. Initially, 43 hybrid strains were used in bioassays against the cotton aphid. Of these strains, 30 induced equal or higher mortality as compared to Vertalec (41.9%). Secondly, 50 hybrid strains were used in bioassays against the greenhouse whitefly. Of these strains, 37 exhibited an equal or higher infection rate as compared to that of Mycotal (36.2%). Finally, 50 hybrid strains were applied to cucumber leaves in order to test strain viability under low humidity...
Palavras-chave: Biological control; Lecanicillium spp; Protoplast fusion; Screening; Verticillium lecanii.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2587
Registros recuperados: 10
Primeira ... 1 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional