Consulta: subjectFacets:"Zymography"
Registros recuperados: 2
Data/hora: 27/07/2024 02:11:38

Partial Purification and Characterization of β-glucosidase fromMonascus sanguineus
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Dikshit,Rashmi; Tallapragada,Padmavathi.
The aim of the present work was to study the production and characterization of β-glucosidase from Monascus sanguineus. Agro-waste residues were screened to obtain the maximum yield of enzyme. Jack fruit seed was the best substrate for enzyme production. Studies on the optimization of pH and temperature showed acidic pH favorable for enzymatic activity, whereas the optimum temperature was 60°C. Enzyme kinetics studies with different concentration of pNPG showed the calculated value of Km approximately 0.89 mM with the non-linear regression and 0.98 mM with the linear regression techniques. The enzyme was predominantly inhibited by KCl (69.8%) and moderately inhibited by CaCl2(14.8%). Studies on the sensitivity for glucose showed that after 100 mM...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zymography; Agro-waste; Kinetics; Monascus sanguineus; PNPG.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200185

Expression of matrix metalloproteases-2 and -9 in horse hoof laminae after intestinal obstruction, with or without Hydrocortisone treatment
Provedor de dados: Ciência Rural
Autores: Laskoski,Luciane Maria; Valadão,Carlos Augusto Araújo; Vasconcelos,Rosemeri de Oliveira; Ferrucci,Danilo; Silva,Juliete Aparecida Francisco; Machado,Dagmar Ruth Stach; Doria,Renata Gebara Sampaio; Mendonça,Fabio de Sousa.
Twenty horses were used in the experiment, for composed control group, (Cg) instrumented group, (Ig;without intestinal obstruction), treated group (Tg;submitted to intestinal obstruction and hydrocortisone treatment) and non-treated group (Ntg;submitted to intestinal obstruction without treatment). Immunohistochemistry and zymography techniques were used for researches on MMPs 2 and 9 in horse hoof laminae. There was an increase in the expression of MMP-2 in animals of Tg and Ntg. MMP-9 increased on animals from groups Ntg and Ig, however there was no rise of this MMP on the Tg when compared to the other groups in the immunohistochemistry analysis. Based on the results, it was observed that the intestinal injury caused by enterotomy and intestinal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laminitis; Metalloprotease; Horse; Zymography; Immunohistochemistry.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000100012