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Pathogenicity and its mode of action in different sedentary stages of Heterodera glycines (Tylenchida: Heteroderidae) by Verticillium lecanii hybrid strains OAK
SHINYA, Ryoji; AIUCHI, Daigo; KUSHIDA, Atsuhiko; TANI, Masayuki; KURAMOCHI, Katsuhisa; KOIKE, Masanori; 相内, 大吾; 谷, 昌幸; 倉持, 勝久; 小池, 正徳.
The current study was conducted to investigate the pathogenicity and its mode of action by Verticillium lecanii hybrid strains in different sedentary stages of Heterodera glycines. Three different sedentary stages (pale yellow female, yellow brown cyst, and dark brown cyst) of H. glycines were treated with V. lecanii and incubated for 3 weeks on water agar. After 3 weeks of incubation, eggs were investigated for the following parameters: (i) the infection frequencies of eggs, (ii) the number of eggs laid, and (iii) the number of mature and healthy eggs. Subsequently, the fecundity of H. glycines treated with V. lecanii was investigated in greater detail. Consequently, some strains had a greater ability to infect eggs and this significantly reduced the...
Palavras-chave: Soybean cyst nematode; Protoplast fusion; Fecundity; Egg laying; Biological control.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2849
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Variation in growth at different temperatures and production and size of conidia in hybrid strains of Verticillium lecanii (Lecanicillium spp.) (Deuteromycotina: Hyphomycetes) OAK
AIUCHI, Daigo; BABA, Yukiko; INAMI, Keigo; SHINYA, Ryoji; TANI, Masayuki; KOIKE, Masanori; 相内, 大吾; 谷, 昌幸; 小池, 正徳.
In a previous study, we confirmed alterations in several fungal traits in hybrid strains of Verticillium lecanii generated via protoplast fusion. In the present study, we surveyed radial colony growth in different temperature regimes as well as conidia productivity and conidia size of hybrid strains of V. lecanii to elucidate the various primary fungal properties of these strains and to determine the correlation between these properties and virulence to insect pests. Minimum temperature thresholds for all tested strains (except Vertalec®) were below 5°C. Optimal temperatures for colony growth were 20–25°C. Colony growth at high temperature was good or poor depending on the genotype. Temperature and strain significantly affected colony growth. Conidial...
Palavras-chave: Lecanicillium spp; Phenotypic variation; Protoplast fusion; Temperature growth response; Verticillium lecanii.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2848
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エンドファイトとしての昆虫病原糸状菌 (特集:昆虫病原糸状菌研究の現状と展望) OAK
小池, 正徳; 相内, 大吾; KOIKE, Masanori; AIUCHI, Daigo.
https://www.jstage.jst.go.jp/article/konchubiotec/82/3/82_3_169/_article/-char/ja/
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3912
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