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Allelic variation at the Vrn-A1, Vrn-B1, Vrn-D1, Vrn-B3 and Ppd-D1a loci of Pakistani spring wheat cultivars Electron. J. Biotechnol.
Iqbal,Muhammad; Shahzad,Armghan; Ahmed,Iftikhar.
Flowering time in bread wheat (Triticum aestivum L.) is controlled by vernalization and photoperiod response, and earliness per se genes. The genetic basis of flowering time has not been investigated in Pakistani bread wheat. This study was, therefore, conducted to determine the allelic composition at Vrn-A1, Vrn-B1, Vrn-D1, Vrn-B3 and Ppd-D1a loci of 59 Pakistani spring bread wheat cultivars. These cultivars, along with 4 isogenic lines for vernalization genes were characterized with previously reported DNA markers designed for detecting allelic variation at 4 Vrn (Vernalization) and 1 Ppd (Photoperiod) loci. Spring habit Vrn-A1a allele was found in 36% cultivars either alone or with spring habit Vrn-B1 and Vrn-D1 alleles. Two wheat cultivars had the...
Tipo: Journal article Palavras-chave: Photoperiod; Spring growth habit; Triticum aestivum; Vernalization.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100001
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Bioinformatics based structural characterization of glucose dehydrogenase (gdh) gene and growth promoting activity of Leclercia sp. QAU-66 BJM
Naveed,Muhammad; Ahmed,Iftikhar; Khalid,Nauman; Mumtaz,Abdul Samad.
Glucose dehydrogenase (GDH; EC 1.1. 5.2) is the member of quinoproteins group that use the redox cofactor pyrroloquinoline quinoine, calcium ions and glucose as substrate for its activity. In present study, Leclercia sp. QAU-66, isolated from rhizosphere of Vigna mungo, was characterized for phosphate solubilization and the role of GDH in plant growth promotion of Phaseolus vulgaris. The strain QAU-66 had ability to solubilize phosphorus and significantly (p < 0.05) promoted the shoot and root lengths of Phaseolus vulgaris. The structural determination of GDH protein was carried out using bioinformatics tools like Pfam, InterProScan, I-TASSER and COFACTOR. These tools predicted the structural based functional homology of pyrroloquinoline quinone domains...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucose dehydrogenase; Pyrroloquinoline quinone; Leclercia sp; PGPR; Bioinformatics.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200031
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Identification and characterization of rhizospheric microbial diversity by 16S ribosomal RNA gene sequencing BJM
Naveed,Muhammad; Mubeen,Samavia; khan,SamiUllah; Ahmed,Iftikhar; Khalid,Nauman; Suleria,Hafiz Ansar Rasul; Bano,Asghari; Mumtaz,Abdul Samad.
In the present study, samples of rhizosphere and root nodules were collected from different areas of Pakistan to isolate plant growth promoting rhizobacteria. Identification of bacterial isolates was made by 16S rRNA gene sequence analysis and taxonomical confirmation on EzTaxon Server. The identified bacterial strains were belonged to 5 genera i.e. Ensifer, Bacillus, Pseudomona, Leclercia and Rhizobium. Phylogenetic analysis inferred from 16S rRNA gene sequences showed the evolutionary relationship of bacterial strains with the respective genera. Based on phylogenetic analysis, some candidate novel species were also identified. The bacterial strains were also characterized for morphological, physiological, biochemical tests and glucose dehydrogenase (gdh)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhizosphere; Root nodules; 16S rRNA gene sequence; Glucose dehydrogenase; Pyrroloquinolone quinone.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300031
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