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An efficient and rapid DNA minipreparation procedure suitable for PCR/SSR and RAPD analyses in tropical forest tree species BABT
Alzate-Marin,Ana Lilia; Guidugli,Marcela Corbo; Soriani,Hilda Hildebrand; Martinez,Carlos Alberto; Mestriner,Moacyr Antônio.
An efficient and rapid DNA minipreparation modified method for frozen samples was developed for five tropical tree species: Copaifera langsdorffii, Hymenaea courbaril, Eugenia uniflora, Tabebuia roseo alba and Cariniana estrellensis. This procedure that dispenses the use of liquid nitrogen, phenol and the addition of proteinase K, is an adaptation of the CTAB-based DNA extraction method. The modifications included the use of PVP to eliminate the polyphenols, only one chloroform-isoamyl alcohol step and the addition of RNase immediately after extraction with chloroform. The yields of the DNA samples ranged from 25.7 to 42.1 µg from 100 mg leaf tissue. The DNA samples extracted by this method were successfully used for PCR (SSR and RAPD) analyses in these...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Copaifera langsdorffii; Hymenaea courbaril; Eugenia uniflora; Tabebuia roseo alba; Cariniana estrellensis; DNA extraction.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500020
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Co-evolution model of Colletotrichum lindemuthianum (melanconiaceae, melanconiales) races that occur in some Brazilian regions Genet. Mol. Biol.
Alzate-Marin,Ana Lilia; Barros,Everaldo Gonçalves de; Moreira,Maurílio Alves.
Colletotrichum lindemuthianum, the causal agent of anthracnose in the common bean (Phaseolus vulgaris L.), displays a high level of virulence diversity, which explains the large number of existing pathotypes. Several lines of evidence indicate that such diversity is, at least in part, due to plant and pathogen co-evolution. A co-evolution model based on the binary classification of 25 races identified in Brazil by inoculation of differential cultivars and random amplified polymorphic DNA (RAPD) data is proposed. In this model, races 8 and 64 that infected bean cultivar Cornell 49-242 (Are gene) and Mexico 222 (Mexico I gene) are considered to be sources of two important evolutionary routes. Inferences about undescribed races from Brazil could be made.
Tipo: Info:eu-repo/semantics/other
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100022
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Diagnostic fingerprints ISSR/SSR for tropical leguminous species Stylosanthes capitata and Stylosanthes macrocephala Scientia Agricola
Alzate-Marin,Ana Lilia; Costa-Silva,Carolina; Rivas,Priscila Marlys Sá; Bonifacio-Anacleto,Fernando; Santos,Leticia Gobett; Moraes Filho,Rômulo Maciel de; Martinez,Carlos Alberto.
ABSTRACT: Stylosanthes capitata Vogel and Stylosanthes macrocephala M.B.Ferreira & Sousa Costa are two forage leguminous species of agronomic importance for animal husbandry in tropical environments. The physical mixture of both species (80 % S. capitata and 20 % S. macrocephala) comprises the commercial cultivar “Estilosantes Campo Grande”. However, proximity of fields for seed production may contaminate seed lots, compromising seeds quality. The combined use of dominant and co-dominant molecular markers is an appropriate strategy to certificate genetic purity and perform diversity studies of cultivars. In this research, a set of ISSR (Inter-Simple Sequence Repeat polymorphic DNA) and SSR (Simple Sequence Repeat polymorphic DNA) molecular...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic purity; Genetic diversity; Seed production.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000301101
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Diversidade genética, endogamia e fluxo gênico em pequena população fragmentada de Copaifera langsdorffii Rev. Bras. Bot.
Carvalho,Ana Cristina Magalhães de; Moraes,Selma Maria Bozite de; Stranghetti,Valéria; Alzate-Marin,Ana Lilia; Sebbenn,Alexandre Magno.
O objetivo deste trabalho foi investigar a taxa de imigração e a distância de dispersão de pólen e sementes em uma pequena população fragmentada de Copaifera langsdorffii Desf., com base no polimorfismo de oito locos microssatélites. Foram mapeadas e genotipadas todas as 47 árvores adultas e 65 subadultas existentes no fragmento. Adultos tinham um total número menor de alelos (142) do que subadultos (164), sendo 20 alelos exclusivos aos adultos e 41 aos subadultos. Adultos tinham significativamente menor diversidade genética e maior endogamia (Â = 17,75 ± 0,51; Âe = 8,97 ± 0,27; ^F = 0,191 ± 0,017) do que subadultos (Â = 20,5 ± 0,65; Âe = 10,86 ± 0,29; ^F = 0,119 ± 0,013). O poder de exclusão do primeiro parente foi alto para o conjunto de locos nos...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Análise de parentesco; Espécies arbóreas tropicais; Tamanho efetivo.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042010000400008
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Genetic characterization of 12 heterologous microsatellite markers for the giant tropical tree Cariniana legalis (Lecythidaceae) Genet. Mol. Biol.
Guidugli,Marcela Corbo; Accoroni,Klaus Alvaro Guerrieri; Mestriner,Moacyr Antonio; Contel,Eucleia Primo Betioli; Martinez,Carlos Alberto; Alzate-Marin,Ana Lilia.
Twelve microsatellite loci previously developed in the tropical tree Cariniana estrellensis were genetically characterized in Cariniana legalis. Polymorphisms were assessed in 28 C. legalis individuals found between the Pardo and Mogi-Guaçu River basins in the state of São Paulo, Brazil. Of the 12 loci, 10 were polymorphic and exhibited Mendelian inheritance. The allelic richness at each locus ranged from 2-11, with an average of 7 alleles per locus, and the expected heterozygosity ranged from 0.07-0.88. These loci showed a high probability of paternity exclusion. The characteristics of these heterologous microsatellite markers indicate that they are suitable tools for investigating questions concerning population genetics in C. legalis.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Jequitibá rosa; Population genetics; SSR markers; Transferability.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100022
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Genetic variability and pedigree analysis of Brazilian common bean elite genotypes Scientia Agricola
Alzate-Marin,Ana Lilia; Costa,Márcia Regina; Sartorato,Aloísio; Del Peloso,María José; Barros,Everaldo Gonçalves de; Moreira,Maurilio Alves.
Genetic diversity is essential for any breeding program. However, breeders tend to concentrate on specific genotypes, which combine traits of interest and may be used as progenitors in several breeding programs. Common bean (Phaseolus vulgaris L.) breeding programs are not different in this sense. In this study, the genetic diversity of 21 common bean elite lines from the Bean Regional Trials conducted by the Embrapa Rice and Bean Research Center was evaluated using the Random Amplified Polymorphic DNA (RAPD) and pedigree analyses. Based on genetic dissimilarity, three groups were defined: group I - lines 1, 9 and 10, with low genetic distances among them (0.00 to 0.06), originated from 11 Mesoamerican parents; group II - 17 lines with genetic distances...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; Andean cultivars; Mesoamerican cultivars; RAPD molecular markers; Genetic distances.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000200012
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Identification of a RAPD marker linked to the Co-6 anthracnose resistant gene in common bean cultivar AB 136 Genet. Mol. Biol.
Alzate-Marin,Ana Lilia; Menarim,Henrique; Chagas,José Mauro; Barros,Everaldo Gonçalves de; Moreira,Maurilio Alves.
The pathogenic variability of the fungus Colletotrichum lindemuthianum represents an obstacle for the creation of resistant common bean (Phaseolus vulgaris L.) varieties. Gene pyramiding is an alternative strategy for the development of varieties with durable resistance. RAPD markers have been proposed as a means to facilitate pyramiding of resistance genes without the need for multiple inoculations of the pathogens. The main aims of this work were to define the inheritance pattern of resistance present in common bean cultivar AB 136 in segregating populations derived from crosses with cultivar Rudá (susceptible to most C. lindemuthianum races) and to identify RAPD markers linked to anthracnose resistance. The two progenitors, populations F1 and F2, F2:3...
Tipo: Info:eu-repo/semantics/article
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300023
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Seleção assistida por marcadores moleculares visando ao desenvolvimento de plantas resistentes a doenças, com ênfase em feijoeiro e soja Trop. Plant Pathol.
Alzate-Marin,Ana Lilia; Cervigni,Gerardo D. L.; Moreira,Maurilio A.; Barros,Everaldo G..
A transferência de alelos de resistência a doenças em plantas pode ser facilitada pelo uso de marcadores moleculares do DNA. Se proximamente ligados a alelos de resistência, eles podem ser usados na seleção assistida por marcadores (S.A.M.). Uma aplicação concreta dos marcadores na S.A.M. é durante o processo de piramidação de alelos de resistência. Por meio da S.A.M., em três gerações de retrocruzamento, o Programa de Melhoramento do Feijoeiro do BIOAGRO, Universidade Federal de Viçosa (Minas Gerais, Brasil), obteve linhagens de feijoeiro (Phaseolus vulgaris) com características fenotípicas similares às da cultivar Rudá (recorrente), contendo alelos de resistência à antracnose, ferrugem e mancha-angular. No momento, sementes das linhagens RC3F4,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Melhoramento de plantas; Piramidação; Culturas anuais; Glycine max; Phaseolus vulgaris.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400001
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Transferability and characterization of microsatellite markers in two Neotropical Ficus species Genet. Mol. Biol.
Nazareno,Alison Gonçalves; Pereira,Rodrigo Augusto Santinelo; Feres,Juliana Massimino; Mestriner,Moacyr Antônio; Alzate-Marin,Ana Lilia.
Microsatellite markers were transferred and characterized for two Neotropical fig tree species, Ficus citrifolia and Ficus eximia. Our study demonstrated that microsatellite markers developed from different subgenera of Ficus can be transferred to related species. In the present case, 12 of the 15 primer pairs tested (80%) were successfully transferred to both of the above species. Eleven loci were polymorphic when tested across 60 F. citrifolia and 60 F. eximia individuals. For F. citrifolia, there were 4 to 15 alleles per locus, whereas expected heterozygosities ranged from 0.31 to 0.91. In the case of F. eximia, this was 2 to 12 alleles per locus and expected heterozygosities from 0.42 to 0.87.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ecological genetics; Ficus citrifolia; Ficus eximia; Moraceae; SSRs.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300021
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Use of RAPD-PCR to identify true hybrid plants from crosses between closely related progenitors Braz. J. Genet.
Alzate-Marin,Ana Lilia; Baía,Gilson Soares; Martins Filho,Sebastião; Paula Júnior,Trazilbo José de; Sediyama,Carlos Sigueyuki; Barros,Everaldo Gonçalves de; Moreira,Maurilio Alves.
RAPD-PCR molecular markers were used to identify common bean and soybean hybrid plants derived from crosses between closely related progenitors, with no apparent phenotypic differences. Primers OP-F12 and OP-0O3 were used to identify true hybrids derived from crosses between common bean cultivars Rudá (A 285) and AN 910408, and soybean cultivars Cristalina and Bossier, respectively. Each primer generated one polymorphic DNA band which was present in the male progenitor and absent in the female progenitor. As RAPD bands are normally inherited as dominant characters, the presence of these bands in the F1 plants confirmed their status.
Tipo: Info:eu-repo/semantics/article Palavras-chave: RAPD-PCR; Hybrid plants; Progenitors.
Ano: 1996 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400016
Registros recuperados: 10
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