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Canine ehrlichiosis: clinical, hematological, serological and molecular aspects Ciência Rural
Nakaghi,Andréa Cristina Higa; Machado,Rosangela Zacarias; Costa,Mirela Tinucci; André,Marcos Rogério; Baldani,Cristiane Divan.
The aim of the present study was to compare the direct detection methods of Ehrlichia canis (blood smears and nested PCR), serological tests (Dot-ELISA and Immunofluorescent Antibody Test - IFAT), and demonstrate the most suitable test for the diagnosis of different stages of infection. Blood samples and clinical data were collected from 30 dogs examined at the Veterinary Teaching Hospital, UNESP, Jaboticabal, SP, Brazil. The clinical signs most frequently observed were apathy, anorexia, pale mucous membrane, fever, lymphadenopathy, splenomegaly, hemorrhages and uveitis. Evaluating the humoral immune response, 63.3% of the sera were IFAT positive, while 70% were Dot-ELISA positive. By nestedPCR 53.3% of the samples were positive. Comparing these techniques...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ehrlichia canis; Dog; IFAT; Dot-ELISA; Nested PCR.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000300027
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Evaluation of dot-blot test for serological diagnosis of bovine brucellosis BJM
Bastos,Carla Resende; Mathias,Luis Antonio; Jusi,Márcia Mariza Gomes; Santos,Renata Ferreira dos; Silva,Glaucenyra Cecília Pinheiro da; André,Marcos Rogério; Machado,Rosangela Zacarias; Bürger,Karina Paes.
Abstract The objective of this study was to standardize and validate the dot-blot test for the serological diagnosis of bovine brucellosis, compare the results with those found in the 2-mercaptoethanol (2-ME) and complement fixation test (CF), and estimate the relative sensitivity and specificity of the dot-blot compared to these tests. Fifty bovine blood serum samples were used for the test standardization, and 1315 samples were used for evaluation and comparison between the tests; the results were compared using the Kappa indicator. At the end of standardization, it was established as optimal for the antigen obtained from Brucella abortus B19 after passing through a microorganism rupture process, the blood serum samples diluted at 1:100, and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brucella abortus; Animal health; Zoonoses; 2-Mercaptoethanol; Complement fixation test.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300564
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