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| Weiblen,Carla; Azevedo,Maria Isabel de; Ianiski,Lara Baccarin; Stibbe,Paula Cristina; Pereira,Daniela Isabel Brayer; Zanette,Régis Adriel; Sangioni,Luís Antônio; Rivero,Rodolfo; Santurio,Janio Morais; Botton,Sônia de Avila. |
| ABSTRACT: We aimed to genotype the South American clinical isolates of Pythium insidiosum using the single nucleotide polymorphisms (SNP) of the ribosomal DNA sequences (rDNA). Previously, an SNP-based multiplex-PCR was able to distinguish three different clades of P. insidiosum isolates. Thus, we used this assay to evaluate South American clinical isolates of P. insidiosum (n=32), standard strains from Costa Rica (n=4), Thailand (n=3), Japan (n=1), and India (n=1), a standard strain of Pythium aphanidermatum, and Brazilian environmental isolates of Pythium torulosum, Pythium rhizo-oryzae and Pythium pachycaule voucher (n=3). It was possible to allocate each American P. insidiosum isolate to clade I, the isolates of India, Japan, and Thailand to clade II,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pythium insidiosum; Pythiosis; Molecular detection; Genotype; Single nucleotide polimorphisms. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000100450 |
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| Pereira,Daniela Isabel Brayer; Botton,Sônia de Avila; Azevedo,Maria Isabel de; Monteiro,Danieli Urach; Weiblen,Carla; Machado,Vanessa Schopf; Donatti,Michele Guimarães; Loeck,Alci Enimar. |
| Leaf-cutting ants of the genera Atta and Acromyrmex determine serious agricultural problems and live on symbiosis with Leucoagaricus gongylophorus. The aim of this study is to identify morphological and molecularly, as well as to verify the genotypic variability of the symbiotic fungus cultivated by A. heyeri and A. ambiguus from three different regions of the state of Rio Grande do Sul, Brazil. Fungus gardens were collected and fragments of mycelia were grown in selective medium. Total DNA was extracted and amplification of the ITS region was performed by PCR using universal primers. After DNA sequencing, the chromatograms were assembled and phylogenetic analyzes were performed by the Neighbor-Joining method. A total of six isolates of L. gongylophorus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Basidiomycete; Leucoagaricus gongylophorus; Internal transcribed spacer.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000701256 |
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| Pereira,Daniela Isabel Brayer; Oliveira,Luiz Sergio Segala de; Bueno,Andressa; Cavalheiro,Ayrton Sydnei; Schwendler,Stela Elisangela; Azevedo,Maria Isabel de; Eckhardt Júnior,João Carlos; Aguiar,Leila Cristiane; Santurio,Deise Flores; Santurio,Janio Morais; Alves,Sydney Hartz. |
| Descreve-se, neste trabalho, um surto de dermatofitose eqüina no Sul do Brasil. Cinqüenta e oito animais jovens (55,2%) apresentavam lesões clínicas características da enfermidade. O diagnóstico micológico realizado pela presença do fungo nos pêlos, associado às características macro e micromorfológicas das colônias isoladas em meio de cultivo, permitiram a classificação do dermatófito como Trichophyton equinum var. equinum. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eqüinos; Dermatófitos; Tinha; Dermatofitose; Trichophyton equinum var. equinum. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000600028 |
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