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Manutenção in vitro de células IDE8 em dois tipos de soro bovino Arq. Bras. Med. Vet. Zootec.
Bastos,C.V.; Vasconcelos,M.M.C.; Ribeiro,M.F.B.; Passos,L.M.F..
The present study had the objective of defining the culture conditions, optimizing the maintenance and expansion of an IDE-8 cell line in Brazil, with the aim to propose its use as a model for in vitro infection and multiplication of Brazilian strains of rickettsia and other hemoparasites. The supplementation of IDE-8 cells with two distinct fetal bovine sera (a Brazilian and an imported) was evaluated. Culture media were changed weekly and subcultures were carried out every 15 days. The development of cultures and subcultures was evaluated by the percentage of viability and cellular morphology. The results indicate that the imported SFB can be replaced by the Brazilian SFB one, as no significant differences (P<0.05) were seen among culture viabilities.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovino; IDE8; Cultivo in vitro; Células de carrapato; Soro fetal bovino.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000200046
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Retrospective study (1998-2001) on canine ehrlichiosis in Belo Horizonte, MG, Brazil Arq. Bras. Med. Vet. Zootec.
Moreira,S.M.; Bastos,C.V.; Araújo,R.B.; Santos,M.; Passos,L.M.F..
The present work describes a retrospective study of clinical cases of ehrlichiosis in dogs examined from March 1998 to September 2001. From the clinical records with laboratorial confirmation of Ehrlichia canis or E. platys infections, the following parameters were analyzed: demographic aspects (age, race, sex, period of the year and origin), clinical characteristics (body temperature, exposure to ticks and clinical signs), and hematological characteristics (blood cell counts and type of infected cell). A total of 194 clinical records were analyzed, from which 31 animals were infected with E. canis and 21 animals with E. platys. The number of cases of canine ehrlichiosis increased considerably from the year 2000 onwards, and 24.4% of the cases occurred in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Ehrlichia canis; Ehrlichia platys; Epidemiology.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000200003
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