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Registros recuperados: 7
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Adaptation of fluorescent technique for genotyping with new microsatellite markers in common bean PAB
Oblessuc,Paula Rodrigues; Campos,Tatiana de; Cardoso,Juliana Morini Kupper; Sforça,Danilo Augusto; Baroni,Renata Moro; Souza,Anete Pereira de; Benchimol,Luciana Lasry.
Tipo: Info:eu-repo/semantics/article
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000600013
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Efeito xênia em híbridos de milho visando ao aumento da produtividade por meio de marcadores microssatélites Bragantia
Lüders,Reginaldo Roberto; Souza,João Cândido de; Balestre,Marcio; Aguiar,Marcelo Sfeir; Amorim,Edson Perito; Benchimol,Luciana Lasry.
Foram desenvolvidos dois experimentos, um com polinização natural e outro com polinização controlada, em duas localidades do Estado de Minas Gerais (Campo Experimental do Departamento de Biologia da Universidade Federal de Lavras - UFLA e Fazenda Experimental da UFLA, conhecida como "Vitorinha") em 2004/2005, para verificar e quantificar o efeito xênia em milho. Nos dois experimentos, marcadores microssatélites foram utilizados para distinguir grãos de origem cruzada dos resultantes de autopolinização. Para o estudo foram utilizados três híbridos simples, P 30F90, A 2555 e DKB 333 B, realizando todos os cruzamentos possíveis entre eles, incluindo os recíprocos. Nos dois ensaios, avaliaram-se amostras compostas de grãos coletados na região mediana de 10...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays; Efeito xênia; Híbridos simples; Produção de grãos.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052008000300008
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Genetic diversity in common bean accessions evaluated by means of morpho-agronomical and RAPD data Scientia Agricola
Chiorato,Alisson Fernando; Carbonell,Sérgio Augusto Morais; Benchimol,Luciana Lasry; Chiavegato,Marilia Barbosa; Dias,Luiz Antonio dos Santos; Colombo,Carlos Augusto.
Germplasm banks store genotype samples, improved varieties, landraces and wild species, all generically denominated accessions. The importance of characterizing germplasm banks is based on the identification and knowledge of relevant traits for genetic improvement and ex situ germplasm conservation. Thus, the present study had as aim the evaluation of the genetic diversity among 220 accessions of a Brazilian common bean germplasm bank of the "Instituto Agronômico de Campinas" (IAC) by means of 23 morpho-agronomical descriptors and 19 RAPD loci. These accessions correspond to genotypes from the Andean and Middle American gene pool as well as from cultivars derived from common bean improvement programs. The Middle American accessions and the improved...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; Germplasm bank; Botanical descriptors; Multivariate analyses.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000300007
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Genetic diversity of cultivated accessions and wild species of rubber tree using EST‑SSR markers PAB
Perseguini,Juliana Morini Küpper Cardoso; Romão,Lineu Roberto de Castro; Briñez,Boris; Scaloppi Junior,Erivaldo José; Gonçalves,Paulo de Souza; Benchimol,Luciana Lasry.
The objective of this work was to evaluate the efficiency of EST‑SSR markers in the assessment of the genetic diversity of rubber tree genotypes (Hevea brasiliensis) and to verify the transferability of these markers for wild species of Hevea. Forty‑five rubber tree accessions from the Instituto Agronômico (Campinas, SP, Brazil) and six wild species were used. Information provided by modified Roger's genetic distance were used to analyze EST‑SSR data. UPGMA clustering divided the samples into two major groups with high genetic differentiation, while the software Structure distributed the 51 clones into eight groups. A parallel could be established between both clustering analyses. The 30 polymorphic EST‑SSRs showed from two to ten alleles and were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fingerprinting analysis; Genetic structure; Functional molecular markers; Polymorphism information content; Transferability.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000800008
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Microsatellite-assisted backcross selection in maize Genet. Mol. Biol.
Benchimol,Luciana Lasry; Souza Jr.,Cláudio Lopes de; Souza,Anete Pereira de.
A microsatellite marker (SSR) was chosen to simulate a target allele and three criteria (02, 04 and 06 markers per chromosome) were tested to evaluate the most efficient parameters for performing marker-assisted backcross (MAB) selection. We used 53 polymorphic SSRs to genotype 186 BC1 maize (Zea mays L.) plants produced by crossing the inbred maize lines L-08-05 (donor parent) and L-14-4B (recurrent parent). The second backcross (BC2) generation was produced with 180 plants and screened with markers which were not recovered from the first backcross (BC1) generation. A total of 480 plants were evaluated in the third backcross (BC3) generation from which 48 plants were selected for parental genotype recovery. Recurrent genotype recovery averages in three...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Maize; Marker-assisted backcrossing (MAB); Marker-assisted selection; Microsatellites.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500022
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Microsatellites for genetic studies and breeding programs in common bean PAB
Campos,Tatiana de; Benchimol,Luciana Lasry; Carbonell,Sérgio Augusto Moraes; Chioratto,Alisson Fernando; Formighieri,Eduardo Fernandes; Souza,Anete Pereira de.
Twenty microsatelitte loci were identified and characterized in common bean. Microsatellites were tested in 14 genotypes. The allele number ranged from 1 to 3, and the polymorphism information content (PIC) was between 0.14 and 0.65. These polymorphic markers are available to be used for breeding programs.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; SSR; Molecular markers.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000400018
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New microsatellite markers developed from an enriched microsatellite common bean library PAB
Cardoso,Juliana Morini Kupper; Oblessuc,Paula Rodrigues; Campos,Tatiana de; Sforça,Danilo Augusto; Carbonell,Sérgio Augusto Moraes; Chioratto,Alisson Fernando; Formighieri,Eduardo Fernandes; Souza,Anete Pereira de; Benchimol,Luciana Lasry.
The objective of this work was to develop new microsatellite markers in common bean. Ninety nine new microsatelitte loci were developed from a microsatellite enriched library for (CT)8 and (GT)8 motifs, from CAL-143 line. The majority of microsatellite sequences (51%) was related to cellular metabolism. The remaining sequences were associated to transcription functions. Only 17.2% of the sequences presented some level of similarity with other plant species genes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; Molecular markers; Plant breeding; QTL; SSR.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000700019
Registros recuperados: 7
Primeira ... 1 ... Última
 

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