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| Benedito,Vagner Augusto; Mourão Filho,Francisco de Assis Alves; Mendes,Beatriz Madalena Januzzi. |
| Com o objetivo de produzir calos embriogênicos de citros, óvulos abortados de frutos maduros de 6 variedades de laranja doce (Citrus sinensis L. Osbeck), Bahia Cabula, Baianinha, Hamlin, Orvalho de Mel, Rubi e Valência foram introduzidos em meio de cultivo MT modificado com adição de extrato de malte, com e sem adição de 5 mg L-1 benziladenina (BA). Os calos obtidos das variedades Bahia Cabula, Orvalho de Mel, Rubi e Valência foram cultivados em meio basal MT, contendo os carboidratos maltose, galactose, lactose, glicose ou sacarose nas concentrações de 18; 37; 75; 110 e 150 mM para a indução da embriogênese somática. Os calos destas mesmas variedades foram submetidos ao isolamento de protoplastos, com o uso de 3 soluções enzimáticas.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus sinensis; Cultura de tecido. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000100007 |
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| Zsögön,Agustin; Lambais,Marcio Rodrigues; Benedito,Vagner Augusto; Figueira,Antonio Vargas de Oliveira; Peres,Lázaro Eustáquio Pereira. |
| Plant hormones are likely key regulators of arbuscular mycorrhizae (AM) development. However, their roles in AM are not well known. Here mutants in five hormone classes introgressed in a single tomato (Lycopersicon esculentum Mill. Syn Solanum lycopersicum L.) background (cv. Micro-Tom) were used to determine their effects on AM development and the expression of defense-related genes (chitinases and b-1,3-glucanases) in roots. Under low P conditions, mutant epinastic (epi) and Never ripe (Nr), ethylene overproducer and low sensitivity, respectively, had the intraradical colonization by Glomus clarum highly inhibited, as compared to the control Micro-Tom (MT). No significant alterations in fungal colonization were observed in mutants affecting other hormone... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lycopersicon; Micro-Tom; Defense-related genes; Hormones; Phosphate. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000300006 |
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| Scotton,Danielle C; Benedito,Vagner Augusto; Molfetta,Jeanne B de; Rodrigues,Benedita Inês FP; Tulmann-Neto,Augusto; Figueira,Antonio. |
| Garlic cultivars are sexually sterile under standard growth conditions, with direct implications for commercial production costs as well as breeding programs. Garlic is propagated commercially via bulblets, which facilitates disease transmission and virus load accumulation over vegetative generations. Tissue culture produces virus-free clones that are more productive, while keeping the desired traits of the cultivar. Consequently, this technique allows studies of garlic genetics as well as guarantees genetic conservation of varieties. We aimed at analyzing the in vitro regeneration of eight marketable cultivars of garlic using root segments as explants. For each genotype, bulblet-derived explants were isolated and introduced into MS medium supplemented... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allium sativum; Bulb; Callus induction; Organogenesis; Regeneration; Tissue culture. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000100013 |
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