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Agronomic performance, chromosomal stability and resistance to velvetbean caterpillar of transgenic soybean expressing cry1Ac gene PAB
Homrich,Milena Schenkel; Passaglia,Luciane Maria Pereira; Pereira,Jorge Fernando; Bertagnolli,Paulo Fernando; Salvadori,José Roberto; Nicolau,Marcio; Kaltchuk-Santos,Eliane; Alves,Laura Bianchessi; Bodanese-Zanettini,Maria Helena.
The objective of this work was to analyze the agronomic performance and chromosomal stability of transgenic homozygous progenies of soybean [Glycine max (L.) Merrill.], and to confirm the resistance of these plants against Anticarsia gemmatalis. Eleven progenies expressing cry1Ac, hpt and gusA genes were evaluated for agronomic characteristics in relation to the nontransformed parent IAS 5 cultivar. Cytogenetical analysis was carried out on transgenic and nontransgenic plants. Two out of the 11 transgenic progenies were also evaluated, in vitro and in vivo, for resistance to A. gemmatalis. Two negative controls were used in resistance bioassays: a transgenic homozygous line, containing only the gusA reporter gene, and nontransgenic 'IAS 5' plants. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anticarsia gemmatalis; Glycine max; Plant resistance to insects; Gene transfer.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000700003
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Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent PAB
Poersch-Bortolon,Liane Balvedi; Scagliusi,Sandra Maria Mansur; Yamazaki-Lau,Elene; Bodanese-Zanettini,Maria Helena.
Abstract The objective of this work was to analyze the androgenic response of Brazilian wheat genotypes to different pretreatments of the spikes, prior to the culture of isolated microspores, and to the effect of a gelling agent in the induction culture medium. Five genotypes were evaluated for embryo formation, green plant regeneration, and spontaneous chromosome duplication. Wheat spikes were subjected to two pretreatments: cold, at 4ºC for 21 days; and 2-hydroxynicotinic acid, at 32ºC for two days. Culture media were evaluated with or without Ficoll as a gelling agent. Cold produced more embryos and green plants than the chemical pretreatment in four out of five genotypes. Only two genotypes treated with 2-hydroxynicotinic acid were able to produce...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Albinism; Androgenesis; Doubled haploid; Isolated microspore culture; Recalcitrance.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016001101839
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Association between floral bud size and developmental stage in soybean microspores BABT
Lauxen,Mozart da Silva; Kaltchuk- Santos,Eliane; Hu,Ching -yeh; Callegari- Jacques,Sidia Maria; Bodanese-Zanettini,Maria Helena.
This study was carried out to establish the association between floral bud size and the corresponding microspore developmental stages for Brazilian soybean cultivars. Microspore developmental stage distributions were examined in young buds from cv Década, IAS5 and RS7. The data indicated that for a given bud-size group, the microspores of different cultivars were at different developmental stages, with cv RS7 and Década distributed at the youngest and cv IAS5 at the most advanced stages. Microspore stages distribution were also compared among the ten anthers of the same bud of the above cultivars. The ten anthers from a given bud were clearly distributed at different developmental stages. Caution should be exercised when adopting the standard anther...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Soybean; Microsporogenesis; Anther culture.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400004
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Detection of cry1 genes in Bacillus thuringiensis isolates from South of Brazil and activity against Aanticarsia gemmatalis (Lepidoptera:Noctuidae) BJM
Bobrowski,Vera Lucia; Pasquali,Giancarlo; Bodanese-Zanettini,Maria Helena; Fiuza,Lidia Mariana.
The bacterium Bacillus thuringiensis (Bt) is characterized by its ability to produce proteic crystalline inclusions during sporulation. Cry1 protein has insecticidal activity and is highly specific to certain insects and not toxic to unrelated insects, plants or vertebrates. In this work, the patogenicity of twelve Bt isolates was tested against Anticarsia gemmatalis, one of the most important insect pests of soybeans. Spore-crystal complex was applied to the surface of artificial diets and the mortality of A. gemmatalis larvae was assessed seven days after each treatment. When compared to a control Bt isolate known by its high toxicity to A. gemmatalis larvae, four novel Bt isolates exhibited even higher toxic activities against the insect, resulting in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus thuringiensis; Velvetbean caterpillar; Anticarsia gemmatalis; Cry1 gene; Delta-endotoxin.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200006
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Evaluation of gelling agents on anther culture: response of two soybean cultivars BABT
Cardoso,Milena Barcelos; Bodanese-Zanettini,Maria Helena; Mundstock,Elsa Cristina de; Kaltchuk-Santos,Eliane.
Anthers of two soybean cultivars were cultured in B5 long basal culture media gelled with agarose or PhytagelTM. Cytological examinations of the anthers were carried out during the first 45 days of culture to assay the viability and developmental stage of microspores. Frequency of callus formation was recorded at 45 days of culture. The analysis of variance of the microspore viability assay showed significant Cultivar X Gelling Agent X Day of Culture interactions. The frequencies of viable microspores decreased significantly with time of culture, within each cultivar and gelling agent tested. The interaction Day X Cultivar was significant for the frequencies of binucleate symmetrical grains and multinucleate/multicellular structures. The effect of gelling...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anther culture; Glycine max; Culture media; Binucleate pollen grains; Multinucleate/multicellular structures; Callus formation.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700004
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Gene expression analysis reveals important pathways for drought response in leaves and roots of a wheat cultivar adapted to rainfed cropping in the Cerrado biome Genet. Mol. Biol.
Poersch-Bortolon,Liane Balvedi; Pereira,Jorge Fernando; Nhani Junior,Antonio; Gonzáles,Hebert Hernán Soto; Torres,Gisele Abigail Montan; Consoli,Luciano; Arenhart,Rafael Augusto; Bodanese-Zanettini,Maria Helena; Margis-Pinheiro,Márcia.
Abstract Drought limits wheat production in the Brazilian Cerrado biome. In order to search for candidate genes associated to the response to water deficit, we analyzed the gene expression profiles, under severe drought stress, in roots and leaves of the cultivar MGS1 Aliança, a well-adapted cultivar to the Cerrado. A set of 4,422 candidate genes was found in roots and leaves. The number of down-regulated transcripts in roots was higher than the up-regulated transcripts, while the opposite occurred in leaves. The number of common transcripts between the two tissues was 1,249, while 2,124 were specific to roots and 1,049 specific to leaves. Quantitative RT-PCR analysis revealed a 0.78 correlation with the expression data. The candidate genes were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 454 sequencing; Candidate genes; RT-qPCR; Triticum aestivum; Water deficit.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400629
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Genes de Bacillus thuringiensis: uma estratégia para conferir resistência a insetos em plantas Ciência Rural
Bobrowski,Vera Lucia; Fiuza,Lidia Mariana; Pasquali,Giancarlo; Bodanese-Zanettini,Maria Helena.
Os insetos constituem uma das principais causas de danos à produção agrícola no mundo. O controle de insetos tem sido realizado por meio de agroquímicos e, em muito menor escala, pelo emprego de inseticidas biológicos. As plantas transgênicas resistentes a insetos representam uma nova alternativa no combate aos insetos-praga das lavouras. A bactéria entomopatogênica Bacillus thuringiensis Berlinier (Bt) é a fonte dos genes de resistência nas chamadas plantas-Bt, produzidas comercialmente. No presente trabalho de revisão, são abordados os aspectos relacionados à bactéria Bt como fonte de genes de resistência a insetos-pragas, plantas geneticamente modificadas, vantagens do uso de plantas-Bt, bem como perspectivas dessa ferramenta biotecnológica.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus thuringiensis; Bt; Plantas transgênicas; Resistência a insetos; Insetos-praga; Delta-endotoxina.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000500008
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Identification and in silico characterization of soybean trihelix-GT and bHLH transcription factors involved in stress responses Genet. Mol. Biol.
Osorio,Marina Borges; Bücker-Neto,Lauro; Castilhos,Graciela; Turchetto-Zolet,Andreia Carina; Wiebke-Strohm,Beatriz; Bodanese-Zanettini,Maria Helena; Margis-Pinheiro,Márcia.
Environmental stresses caused by either abiotic or biotic factors greatly affect agriculture. As for soybean [Glycine max (L.) Merril], one of the most important crop species in the world, the situation is not different. In order to deal with these stresses, plants have evolved a variety of sophisticated molecular mechanisms, to which the transcriptional regulation of target-genes by transcription factors is crucial. Even though the involvement of several transcription factor families has been widely reported in stress response, there still is a lot to be uncovered, especially in soybean. Therefore, the objective of this study was to investigate the role of bHLH and trihelix-GT transcription factors in soybean responses to environmental stresses. Gene...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Drought; Gene expression; Glycine max; Phylogeny; Plant-microbe interactions.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200005
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Induction and identification of polyploids in Cattleya intermedia Lindl. (orchidaceae) by in vitro techniques Ciência Rural
Silva,Paulo Artur Konzen Xavier de Mello e; Callegari-Jacques,Sídia; Bodanese-Zanettini,Maria Helena.
The effect of colchicine on the protocorm-like bodies of Cattleya intermedia L. (Orchidaceae) cultured in vitro was studied. Different concentrations of the drug (0.00, 0.05, 0.10 and 0.20%) as well as two times of culture (4 or 8 days) were tested to determine the best treatment for the induction of tetraploid plants. Of the 3 colchicine levels applied, 0.05 and 0.10% of drug treatments appeared to be effective on the production of mixoploids and tetraploids and could be further used in breeding programs. The suitability of stomatal area and stomata density as criteria for the distinction between diploids and tetraploids was also tested. The results suggested that tetraploid plants could be identified with a fair amount of certainty when the screening was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattleya; Colchicine; Chromosome number; Orchid improvement; Polyploidy; Stomata analysis.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000100017
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Influence of antibiotics on embryogenic tissue and Agrobacterium tumefaciens suppression in soybean genetic transformation Bragantia
Wiebke,Beatriz; Ferreira,Fabricio; Pasquali,Giancarlo; Bodanese-Zanettini,Maria Helena; Droste,Annette.
The influence of different antibiotic treatments in soybean genetic transformation was evaluated. First, an assay was performed to verify how different antibiotic treatments affect soybean embryogenic tissues. The effect of carbenicillin at 500 mg L-1 was genotype-dependent. This antibiotic did not affect embryo survival of cv. IAS5, but a three-fold increase of embryo proliferation was observed for cv. Bragg, when compared to the control. On the other hand, cefotaxime at 350 and 500 mg L-1 caused death of embryogenic tissues of both cultivars. Finally, the association of cefotaxime (250 mg L-1) + vancomycin (250 mg L-1) did not affect negatively the somatic embryos of tested cultivars until 63 days of treatment. Thereafter, a second experiment was carried...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agrobacterium tumefaciens; Carbenicillin; Cefotaxime; Genetic transformation; Glycine max; Vancomycin.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052006000400002
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Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling BABT
Cardoso,Milena Barcelos; Kaltchuk-Santos,Eliane; Mundstock,Elsa Cristina de; Bodanese-Zanettini,Maria Helena.
Anthers obtained from flowers buds of soybean cultivar IAS-5 were cultured in two basal culture media (B5 and B5 long). Cytological examinations of the in vitro anthers were performed during the first 20 days of culture to assay the viability (by propionic-carmine and fluorescein diacetate tests) and the stage of development of pollen grains. The frequencies of viable pollen grains varied significantly between bud sizes on the propionic-carmine analysis. The basal culture media and bud size had no clear effect on the frequencies of binucleate symmetrical and multinucleate pollen grains. Chromosome counts of metaphasic microspores throughout the culture period showed microspores with higher ploidy level in addition to normal chromosome number (n=20).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; FDA; Binucleate pollen grains; Multinucleate pollen grains; Spontaneous diploidization.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500005
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Isolation and characterization of two plant growth-promoting bacteria from the rhizoplane of a legume (Lupinus albescens) in sandy soil Rev. Bras. Ciênc. Solo
Giongo,Adriana; Beneduzi,Anelise; Ambrosini,Adriana; Vargas,Luciano Kayser; Stroschein,Marcos Roberto; Eltz,Flávio Luiz; Bodanese-Zanettini,Maria Helena; Passaglia,Luciane Maria Pereira.
Two bacterial strains that amplified part of the nifH gene, RP1p and RP2p, belonging to the genus Enterobacter and Serratia, were isolated from the rhizoplane of Lupinus albescens. These bacteria are Gram-negative, rod-shaped, motile, facultative anaerobic, and fast-growing; the colonies reach diameters of 3-4 mm within 24 h of incubation at 28 ºC. The bacteria were also able to grow at temperatures as high as 40 ºC, in the presence of high (2-3 % w/v) NaCl concentrations and pH 4 -10. Strain RP1p was able to utilize 10 of 14 C sources, while RP2p utilized nine. The isolates produced siderophores and indolic compounds, but none of them was able to solubilize phosphate. Inoculation of L. albescens with RP1p and RP2p strains resulted in a significant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Indolic compounds; Siderophores; Inoculation experiment.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000200009
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Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains BABT
Rodrigues,Lia Rosane; Forte,Bianca de Camargo; Bodanese-Zanettini,Maria Helena.
In the last three decades, research on soybean microspore embryogenesis was restricted to anther culture, which presents limitations such as the small number of responsive microspores and the high embryogenic potential of sporophytic tissues. Therefore, a sequence of studies was performed to establish appropriate conditions for the isolation and culture of soybean microspores and pollen grains as an alternative to anther culture. First, a pollen and microspore isolation technique was developed using floral buds from four soybean cultivars (Bragg, IAS 5, MG/BR-46 Conquista and BRSMT Uirapuru). This technique allowed the establishment of cultures with satisfactory density and characteristics. Subsequently, different culture conditions were tested. Although...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Androgenesis; Glycine max; Microspore culture; Microspore embryogenesis; Microspore isolation; Soybean.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500002
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Regeneration of soybean via embryogenic suspension culture Scientia Agricola
Droste,Annette; Leite,Paulo César Pimentel; Pasquali,Giancarlo; Mundstock,Elsa Cristina de; Bodanese-Zanettini,Maria Helena.
In an attempt to establish an alternative plant regeneration system for soybean [Glycine max (L.) Merrill] cultivars used in Brazilian breeding programs, ten genotypes were tested for their embryogenic potential. Cotyledons were removed as explants from immature seeds harvested from field-grown plants. After 45 days on induction medium, the number of responding cotyledons and the number of somatic embryos per immature cotyledon were evaluated. The percentage of explants that produced somatic embryos varied from 1 to 70% among cultivars. The average number of somatic embryos produced per cotyledon pair ranged from 0.01 to 10.3 with a mean of 3.4. Suspension cultures were initiated with three Agrobacterium tumefaciens susceptible cultivars. Suspensions were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Somatic embryogenesis; Liquid medium; Plant tissue culture.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162001000400017
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Resistance to Anticarsia gemmatalis Hübner (Lepidoptera, Noctuidae) in transgenic soybean (Glycine max (L.) Merrill Fabales, Fabaceae) cultivar IAS5 expressing a modified Cry1Ac endotoxin Genet. Mol. Biol.
Homrich,Milena Schenkel; Passaglia,Luciane Maria Pereira; Pereira,Jorge Fernando; Bertagnolli,Paulo Fernando; Pasquali,Giancarlo; Zaidi,Mohsin Abbas; Altosaar,Illimar; Bodanese-Zanettini,Maria Helena.
Somatic embryos of the commercial soybean (Glycine max) cultivar IAS5 were co-transformed using particle bombardment with a synthetic form of the Bacillus thuringiensis delta-endotoxin crystal protein gene cry1Ac, the beta-glucuronidase reporter gene gusA and the hygromycin resistance gene hpt. Hygromycin-resistant tissues were proliferated individually to give rise to nine sets of clones corresponding to independent transformation events. The co-bombardment resulted in a co-transformation efficiency of 44%. Many histodifferentiated embryos and 30 well-developed plants were obtained. Twenty of these plants flowered and fourteen set seeds. The integration and expression of the cry1Ac, gusA and hpt transgenes into the genomes of a sample of transformed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anticarsia gemmatalis; Cry1Ac gene; IAS5 cultivar; Insect resistance; Transgenic soybean.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300020
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Screening of Brazilian soybean genotypes with high potential for somatic embryogenesis and plant regeneration PAB
Droste,Annette; Silva,Anelise Machado da; Souza,Iliane Freitas de; Wiebke-Strohm,Beatriz; Bücker-Neto,Lauro; Bencke,Marta; Sauner,Márcia Viviane; Bodanese-Zanettini,Maria Helena.
The aim of this work was to identify Brazilian soybean (Glycine max) genotypes with potential to respond to in vitro culture stimuli for primary somatic embryo induction, secondary embryo proliferation and plant regeneration. Differences among eight tested cultivars were observed at each stage. Two cultivars, IAS-5 and BRSMG 68 Vencedora, were selected for the evaluation of the capacity for embryo differentiation and plant regeneration. These cultivars had high embryo induction frequencies, repetitive embryogenic proliferation, and low precocious embryo germination in the initial experiment. The effect of abscisic acid (ABA) and charcoal addition on plant regeneration was investigated. The addition of ABA to proliferation medium and of ABA and activated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Brazilian cultivars; Embryogenic potential; Genetic engineering; Soybean transformation.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000700012
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Somatic embryogenesis from immature cotyledons of soybean (Glycine max (L.) Merr.): ontogeny of somatic embryos BABT
Santos,Karla Galvão Bezerra dos; Mariath,Jorge Ernesto de Araújo; Moço,Maria Cecília C.; Bodanese-Zanettini,Maria Helena.
Soybean somatic embryos obtained from immature zygotic embryos were histologically analyzed in order to determine possible ontogenetic routes followed by these embryos. The proembryo stage and globular, heart-shaped, torpedo and cotyledonary embryo stages were found, closely resembling the ontogeny of zygotic embryos. However, the absence of a characteristic suspensor as well as the delay in the establishment of inner organization were the main differences between these two embryogenic processes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Ontogeny; Somatic embryo.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100006
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Soybean genetic transformation: a valuable tool for the functional study of genes and the production of agronomically improved plants Genet. Mol. Biol.
Homrich,Milena Schenkel; Wiebke-Strohm,Beatriz; Weber,Ricardo Luís Mayer; Bodanese-Zanettini,Maria Helena.
Transgenic plants represent an invaluable tool for molecular, genetic, biochemical and physiological studies by gene overexpression or silencing, transposon-based mutagenesis, protein sub-cellular localization and/or promoter characterization as well as a breakthrough for breeding programs, allowing the production of novel and genetically diverse genotypes. However, the stable transformation of soybean cannot yet be considered to be routine because it depends on the ability to combine efficient transformation and regeneration techniques. Two methods have been used with relative success to produce completely and stably transformed plants: particle bombardment and the Agrobacterium tumefaciens system. In addition, transformation by Agrobacterium rhizogenes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Transgenic plants; Particle bombardment; Agrobacterium systems; Functional analysis; Genetic improvement.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600015
Registros recuperados: 18
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