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BELESINI, A. A.; TELLES, B. R.; CASTRO, G. M. de; GIACHETTO, P. F.; VANTINI, J. da S.; CARVALHO, F. M. de S.; CARLIN, S. R.; CAZETTA, J. O.; FERRO, M. I. T.; PINHEIRO, D. G.. |
Sugarcane is an important crop, major source of sugar and alcohol, accounting for two-thirds of the world's sugar production. In Brazil, the sugarcane culture has expanded to areas with prolonged drought seasons, which is constraining its production. In order to identify genes and molecular process related to sugarcane drought tolerance, we performed de novo assembly and transcriptome analysis of two sugarcane genotypes, one tolerant and other sensitive to water stress, submitted to three water deficit condition (30, 60 and 90 days). The de novo assembly of leaves transcriptome was performed using short reads from Illumina RNA-Seq platform, which produced more than 1 billion reads, which were assembled into 177,509 and 185,153 transcripts sequences for the... |
Tipo: Anais e Proceedings de eventos |
Palavras-chave: Bioinformática; Cana-de-açúcar; Tolerância à seca; Transcriptoma; Bioinformatics; Sugarcane; Drought tolerance; Water stress; Transcriptomics. |
Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1038946 |
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BELESINI, A. A.; CARVALHO, F. M. S.; TELLES, B. R.; CASTRO, G. M. de; GIACHETTO, P. F.; VANTINI, J. S.; CARLIN, S. D.; CAZETTA, J. O.; PINHEIRO, D. G.; FERRO, M. I. T.. |
ABSTRACT. Sugarcane production is strongly influenced by drought, which is a limiting factor for agricultural productivity in the world. In this study, the gene expression profiles obtained by de novo assembly of the leaf transcriptome of two sugarcane cultivars that differ in their physiological response to water deficit were evaluated by the RNA-Seq method: drought-tolerant cultivar (SP81-3250) and drought-sensitive cultivar (RB855453). For this purpose, plants were grown in a greenhouse for 60 days and were then submitted to three treatments: control (-0.01 to -0.015 MPa), moderate water deficit (-0.05 to -0.055 MPa), and severe water deficit (-0.075 to -0.08 MPa). The plants were evaluated 30, 60, and 90 days after the beginning of treatment.... |
Tipo: Artigo de periódico |
Palavras-chave: Expressão genética; Cana de açúcar; Deficiência hídrica; Sugarcane; Drought; Gene expression; Saccharum; Water stress. |
Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1082783 |
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HONGO, J. A.; CASTRO, G. M. de; CINTRA, L. C.; ZERLOTINI, A.; LOBO, F. P.. |
Abstract: Background: Detection of genes evolving under positive Darwinian evolution in genome-scale data is nowadays a prevailing strategy in comparative genomics studies to identify genes potentially involved in adaptation processes. Despite the large number of studies aiming to detect and contextualize such gene sets, there is virtually no software available to perform this task in a general, automatic, large-scale and reliable manner. This certainly occurs due to the computational challenges involved in this task, such as the appropriate modeling of data under analysis, the computation time to perform several of the required steps when dealing with genome-scale data and the highly error-prone nature of the sequence and alignment data structures needed... |
Tipo: Artigo de periódico |
Palavras-chave: Potion; Evolução Darwiniana; Comparative genomics; Positive selection; Gene; Fenótipo; Pesquisa; Genomics; Phylogeny; Genes; Genome. |
Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1027912 |
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