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| Registros recuperados: 10 | |
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| Lima,Marcos Antonio Barbosa de; Nascimento,Aline Elesbão do; Souza,Wanderley de; Fukushima,Kazutaka; Campos-Takaki,Galba Maria de. |
| The content of inorganic polyphosphate and the polymeric degree of these compounds were evaluated during the growth of Cunninghamella elegans in medium containing varying orthophosphate (Pi) concentrations. For this purpose, a combination of chemical methods for polyphosphate extraction and ultrastructural cytochemistry were used. The orthophosphate and glucose consumption was also determined during the fungal cultivation. At Pi concentrations of 0.5, 2.5 and 0.0 g/L, the maximum amounts of biomass were 3.18, 3.29 and 0.24 g/L, respectively. During growth the cells accumulated Pi from the medium. At three days of growth the biomass consumed up to 100 and 95% of Pi from the media at initial concentrations of 0.5 and 2.5 g/L, respectively. Polyphosphate was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polyphosphate; Accumulation; Ultrastructural cytochemistry; Cunninghamella elegans; Ortophosfate. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400016 |
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| Stamford,Thayza Christina Montenegro; Stamford,Tânia Lucia Montenegro; Stamford,Newton Pereira; Barros Neto,Benicio de; Campos-Takaki,Galba Maria de. |
| Microbiological processes were used for chitin and chitosan productions by Cunninghamella elegans (UCP 542) grown in a new economic culture medium. The assay was carried out to evaluate the growth of C. elegans using yam bean (Pachyrhizus erosus L. Urban) medium, in different times of growth (24, 48, 72 and 96 hrs), incubated at 28ºC in an orbital shaker at 150 rpm. The lyophilized biomass was determined by gravimetry. The polysaccharides were extracted by alkali-acid treatment, and characterized by infrared spectroscopy, titration and viscosity. C. elegans grown in the yam bean medium and produced higher yields of biomass (24.3 g/ mL) in 96 hrs. The high level was chitosan (66 mg/g), and chitin (440 mg/g) were produced at 48 and 72 hrs of growth,... |
| Tipo: Journal article |
Palavras-chave: Biopolymers; Chitin; Chitosan. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100006 |
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| Franco,Luciana de Oliveira; Maia,Rita de Cássia C.; Porto,Ana Lúcia F.; Messias,Arminda Sacconi; Fukushima,Kazutaka; Campos-Takaki,Galba Maria de. |
| Chitin and chitosan were extracted from mycelial biomass of Cunninghamella elegans and the performance for copper, lead and iron biosorption in aqueous solution was evaluated. The growth curve of C. elegans was accomplished by determination of biomass, pH, glucose and nitrogen consumption. Chitin and chitosan were extracted by alkali-acid treatment and the yields were 23.8 and 7.8%, respectively. For the adsorption analysis, the process of heavy uptake metal sorption was evaluated using polysaccharides solutions (1% w/v). The rate of metallic biosorption was dependent upon the concentration and pH of metal solutions, and the best results were observed with pH 4.0. Chitosan showed the highest affinity for copper and chitin for iron adsorption. The results... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cunninghamella elegans; Chitin; Chitosan; Bioremediation; Biosorption. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200013 |
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| Vance-Harrop,Mabel H.; Gusmão,Norma B. de; Campos-Takaki,Galba Maria de. |
| Candida lipolytica IA 1055 produced extracellular biosurfactants with emulsification activity by fermentation using babassu oil and D-glucose as carbon sources. Natural seawater diluted at 50% supplemented with urea, ammonium sulfate, and phosphate was used as economic basal medium. The best results were achieved with the YSW-B2 medium, which contained urea, ammonium sulfate, and babassu oil and with YSW-B3 medium, which contained urea, ammonium sulfate, phosphate, and babassu oil, kept under fed batch fermentation for 60 hours with 5% of babassu oil. For the two media, the maximum specific growth rates were 0.02 h-1 and 0.04 h-1; the generation times were 34.6 h-1 and 17.3 h-1, and the emulsification activities were 0.666 and 0.158 units, respectively.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioemulsifier; Candida lipolytica; Seawater; Babassu oil. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200006 |
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| Shari'a,Allana Elesbão do Nascimento; Nascimento,Aline Elesbão do; Lima,Marcos Antonio Barbosa de; Campos-Takaki,Galba Maria de; Souza,Wanderley de. |
| The inorganic phosphorus content, and distribution, structure and localization of polyphosphate in mycelia of zygomycetous fungi was evaluated. Ultrastructural cytochemistry was successfully used to identifying the localization and distribution of polyphosphate in Absidia cylindrospora, Gongronella butleri and Mucor javanicus. The results revealed differences in the cytochemical staining pattern in all species studied and a uniform labeling on the cellular surface (cell wall and citoplasmic membrane). Reaction products were observed in intracellular structures and cellular membrane. Intracellular staining was observed in trabecullar, vacuolar and vesicular structures, in dense bodies and in the cytoplasm. However, the cytochemical staining intensity varied... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ultrastructural cytochemistry; Polyphosphate; Zygomycetes. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200004 |
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| Andrade,Vânia Sousa; Sarubbo,Leonie Asfora; Fukushima,Kasutaka; Miyaji,Makoto; Nishimura,Kazuko; Campos-Takaki,Galba Maria de. |
| Recently, some Mucorales species have been reported as protease producers. The production of extracellular proteases by Mucor circinelloides using glucose as substrate was studied. Experiments were carried out with different D-glucose concentrations (40, 60 and 80 g/L). Biomass, pH and protease activity were determined. Although biomass production had reached best yields for the medium containing D-glucose in a concentration of 80 g/L, the enzymatic production was higher when the substrate concentration was reduced to 40 g/L. The yield factor for product on cell growth and the yield factor for product on carbon substrate were higher when the microorganism grew in medium containing 40 g/L glucose. The kinetics parameters suggest that this strain seems to be... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Extracellular proteases; D-glucose; Substrate; Mucor circinelloides. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200002 |
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| Registros recuperados: 10 | |
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