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Endophytic fungi from Brachiaria grasses in Brazil and preliminary screening of Sclerotinia sclerotiorum antagonists Scientia Agricola
Gama,Danielly de Souza; Santos,Ítalo Augusto Férrer Melo; Abreu,Lucas Magalhães de; Medeiros,Flávio Henrique Vasconcelos de; Duarte,Whasley Ferreira; Cardoso,Patrícia Gomes.
ABSTRACT: Fungal endophytes of Brachiaria, a nonhost of Sclerotinia sclerotiorum, may harbor species with antagonistic effects against this plant pathogen. The objective of this work was to investigate the diversity of endophytic fungi associated with different Brachiaria species and hybrids and evaluate their potential to inhibit the plant pathogen S. sclerotiorum. Stem samples from 39 Brachiaria spp. plants were collected in pasture fields and experimental areas of three states of Brazil resulting in 74 endophytes isolated. Twenty-eight species were identified by sequences of the Internal Transcribed Spacer (ITS) and 18S rDNA regions. Paraconiothyrium sp. was the most abundant endophyte, accounting for 24 % (14 isolates) of total, and it was isolated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coniothyrium-like fungi; Urochloa; Tropical forage grasses; Antagonism; White mold.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000300200
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Exopolysaccharides produced by the symbiotic nitrogen-fixing bacteria of leguminosae Rev. Bras. Ciênc. Solo
Bomfeti,Cleide Aparecida; Florentino,Ligiane Aparecida; Guimarães,Ana Paula; Cardoso,Patrícia Gomes; Guerreiro,Mário César; Moreira,Fatima Maria de Souza.
The process of biological nitrogen fixation (BNF), performed by symbiotic nitrogen fixing bacteria with legume species, commonly known as α and β rhizobia, provides high sustainability for the ecosystems. Its management as a biotechnology is well succeeded for improving crop yields. A remarkable example of this success is the inoculation of Brazilian soybeans with Bradyrhizobium strains. Rhizobia produce a wide diversity of chemical structures of exopolysaccharides (EPS). Although the role of EPS is relatively well studied in the process of BNF, their economic and environmental potential is not yet explored. These EPS are mostly species-specific heteropolysaccharides, which can vary according to the composition of sugars, their linkages in a single...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Exopolysaccharides; Nodules; Environmental stress; Soil aggregation; Rhizobia.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832011000300001
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Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential R. Bras. Zootec.
Maia,Natália da Costa; Souza,Patrícia Nirlane da Costa; Godinho,Bárbara Temponi Vilarino; Moreira,Silvino Intra; Abreu,Lucas Magalhães de; Jank,Liana; Cardoso,Patrícia Gomes.
ABSTRACT This study aimed to isolate and identify endophytic fungi from the forage grass P. maximum and evaluate their ability to inhibit the growth of plant pathogenic fungi. One sample from P. purpureum grass was also included. Surface disinfected stem fragments were used for endophytic fungal isolation. One hundred and twenty-six endophytic fungi were isolated, of which 118 were from P. maximum and eight from P. purpureum. Morphological characteristics and internal transcribed spacer (ITS) and 18S (NS) sequence comparisons identified most isolated endophytic fungi as belonging to the phylum Ascomycota, with Sarocladium being the dominant genus. The isolates were subjected to in vitro antagonism tests against pathogenic fungi, and 31 endophytic fungi...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antagonism; Grasses; Molecular identification; Plant pathogens; RDNA.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100303
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Morphological and molecular differentiation of the pectinase producing fungi Penicillium expansum and Penicillium griseoroseum BJM
Cardoso,Patrícia Gomes; Queiroz,Marisa Vieira de; Pereira,Olinto Liparini; Araújo,Elza Fernandes de.
Two species from the genus Penicillium, Penicillium expansum and P. griseoroseum (Brasilian isolates) were characterized morphologic and molecularlly. Morphological variability was detected among isolates in regard to colony morphology and to conidia coloration. The molecular characterization was based on the RAPD markers, telomeric fingerprinting and ITS sequencing. A total of 78 RAPD primers were used and 8 presented differences in band patterns with 54% of the amplified polymorphic fragments. The monomorphic fragments of 600 bp (P. expansum) and 594 bp (P. griseoroseum) were amplified. The only internal transcribed spacer region variation detected between the two species was the additional six initial nucleotides. The analysis by telomeric...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ribossomal DNA; Internal Transcribed Spacer; Subtelomeric Region; Penicillium spp.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100015
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