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A priori estimation of accuracy and of the number of wells to be employed in limiting dilution assays BJMBR
Chaui-Berlinck,J.G.; Barbuto,J.A.M..
The use of limiting dilution assay (LDA) for assessing the frequency of responders in a cell population is a method extensively used by immunologists. A series of studies addressing the statistical method of choice in an LDA have been published. However, none of these studies has addressed the point of how many wells should be employed in a given assay. The objective of this study was to demonstrate how a researcher can predict the number of wells that should be employed in order to obtain results with a given accuracy, and, therefore, to help in choosing a better experimental design to fulfill one's expectations. We present the rationale underlying the expected relative error computation based on simple binomial distributions. A series of simulated in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Limiting dilution assay: accuracy; Wells; Number of; Relative error.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800010
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Further analysis of open-respirometry systems: an a-compartmental mechanistic approach BJMBR
Chaui-Berlinck,J.G.; Bicudo,J.E.P.W..
A system is said to be "instantaneous" when for a given constant input an equilibrium output is obtained after a while. In the meantime, the output is changing from its initial value towards the equilibrium one. This is the transient period of the system and transients are important features of open-respirometry systems. During transients, one cannot compute the input amplitude directly from the output. The existing models (e.g., first or second order dynamics) cannot account for many of the features observed in real open-respirometry systems, such as time lag. Also, these models do not explain what should be expected when a system is speeded up or slowed down. The purpose of the present study was to develop a mechanistic approach to the dynamics of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Open-respirometry systems; Thermodynamic model.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800014
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