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| Degrelle, S.A.; Hennequet-Antier, C.; Chiapello, H.; Piot-Kaminski, K.; Piumi, F.; Robin, S.; Renard, J.P.; Hue, I.. |
| BackgroundGene expression profiling has become a tool of choice to study pathological or developmental questions but in most cases the material is scarce and requires sample amplification. Two main procedures have been used: in vitro transcription (IVT) and polymerase chain reaction (PCR), the former known as linear and the latter as exponential. Previous reports identified enzymatic pitfalls in PCR and IVT protocols; however the possible differences between the sequences affected by these amplification defaults were only rarely explored.ResultsScreening a bovine cDNA array dedicated to embryonic stages with embryonic (n = 3) and somatic tissues (n = 2), we proceeded to moderate amplifications starting from 1 μg of total RNA (global PCR or IVT one round).... |
| Tipo: Journal Article |
Palavras-chave: AMPLIFICATION; IN VITRO TRANSCRIPTION (IVT); POLYMERASE CHAIN REACTION (PCR); GENE EXPRESSION; MICROARRAY. |
| Ano: 2008 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200839ed8f6c&uri=/notices/prodinra1/2009/11/ |
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| Yockteng, R.; Marthey, S.; Chiapello, H.; Gendrault, A.; Hood, M.E.; Rodolphe, F.; Devier, B.; Wincker, P.; Dossat, C.; Giraud. |
| The basidiomycete fungus Microbotryum violaceum is responsible for the anther-smut disease in many plants of the Caryophyllaceae family and is a model in genetics and evolutionary biology. Infection is initiated by dikaryotic hyphae produced after the conjugation of two haploid sporidia of opposite mating type. This study describes M. violaceum ESTs corresponding to nuclear genes expressed during conjugation and early hyphal production.ResultsA normalized cDNA library generated 24,128 sequences, which were assembled into 7,765 unique genes; 25.2% of them displayed significant similarity to annotated proteins from other organisms, 74.3% a weak similarity to the same set of known proteins, and 0.5% were orphans. We identified putative pheromone receptors and... |
| Tipo: Journal Article |
Palavras-chave: SELECTION POSITIVE; BASIDIOMYCETE; EXPRESSION GENIQUE; BANQUE DE GENES; GENOMIQUE MICROBOTRYUM VIOLACEUM. |
| Ano: 2007 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008243f0734&uri=/notices/prodinra1/2009/11/ |
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| Chiapello, H.; Bourgait, I.; Sourivong, F.; Heuclin, G.; Gendrault-Jacquemard, A.; Petit, M.A.; El Karoui, M.. |
| Background :Public databases now contain multitude of complete bacterial genomes, including several genomes of the same species. The available data offers new opportunities to address questions about bacterial genome evolution, a task that requires reliable fine comparison data of closely related genomes. Recent analyses have shown, using pairwise whole genome alignments, that it is possible to segment bacterial genomes into a common conserved backbone and strain-specific sequences called loops.Results :Here, we generalize this approach and propose a strategy that allows systematic and non-biased genome segmentation based on multiple genome alignments. Segmentation analyses, as applied to 13 different bacterial species, confirmed the feasibility of our... |
| Tipo: Journal Article |
Palavras-chave: GENOME BACTERIEN; EVOLUTION DU GENOME; COMPARAISON DE GENOME ENTIER BACTERIAL GENOME; GENOME EVOLUTION. |
| Ano: 2005 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007f72738ba&uri=/notices/prodinra1/2007/08/ |
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