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| Chitarra,Cristiane Silva; Silva,Mayara Inácio Vincenzi da; Brandão,Laila Natasha Santos; Kagueyama,Francielle Cristina; Candido,Stefhano Luis; Rosa,Janaina Marcela Assunção; Nakazato,Luciano; Dutra,Valéria. |
| ABSTRACT: Swine respiratory diseases such as atrophic rhinitis and bronchopneumonia caused by Pasteurella (P.) multocida cause important economic losses to the modern swine industry. The purpose of this study was to characterize P. multocida strains isolated from swine lungs by RAPD (Randomly Amplified Polymorphic DNA) to demonstrate their genetic diversity. Ninety-four samples of fragments from lungs with pneumonia and sixty one samples without pneumonia were collected in slaughterhouses in Mato Grosso during the period from December 2009 to March 2010. Clinical cases in 2012 and 2013 were also included in this study. Among the lung fragments with macroscopic lesions, without macroscopic lesions and clinical samples, 40.42%, 4.49% and 100% were positive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pulmonary pasteurellosis; Swine; Pneumonia; Pasteurella multocida; RAPD. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100119 |
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| Castro,Vanessa Vieira; Ayres,Eveline da Cruz Boa Sorte; Canei,Darlan Henrique; Pereira,Mariana Elisa; Sousa,Valéria Régia Franco; Chitarra,Cristiane Silva; Dutra,Valéria; Nakazato,Luciano; Almeida,Arleana do Bom Parto Ferreira de. |
| ABSTRACT: Canine babesiosis is a common haemoparasitosis in Brazil. Caused by parasites of the genus Babesia, it is transmitted by ixodid ticks and affects domestic and wild canids. The objective of this study was to verify the prevalence of Babesia species (spp.) using molecular methods in dogs living in urban and rural areas of Cuiabá, Mato Grosso State, Brazil, and to identify the main factors associated with infection. A total of 407 samples from 407 dogs were evaluated using a polymerase chain reaction (PCR) technique, among which Babesia species (spp.) was amplified in 10 (2.5%). Although, no statistical association was found among the variables studied (p>0.05), greater positivity was observed in dogs<1 year of age, male sex, those with free... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Babesiosis; Molecular identification; Cuiaba. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000200500 |
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| Witter,Rute; Melo,Andréia Lima Tomé; Pacheco,Thábata dos Anjos; Meneguzzi,Mirella; Boas,Ricardo Vilas; Dutra,Valéria; Nakazato,Luciano; Chitarra,Cristiane Silva; Oliveira,Anderson Castro Soares de; Pacheco,Richard Campos. |
| ABSTRACT: The municipality of Ji-Paraná, Rondônia, is one of the major dairy production areas in the north region of Brazil. Thus, it is important to evaluate infectious agents that have the potential to negatively affect productivity in the industry. The objective of this study was to investigate the prevalence of ' Candidatus Mycoplasma haemobos' by using a PCR-based detection method and correlate this with dairy herd variables (abortion frequency, weak calf birth rate, total cattle number, >24-month-old cow number, farm size, and production system) in family farms of the Ji-Paraná municipality, north region, Brazil. Blood samples were collected from 320 dairy cows located across 64 farms (i.e., five animals per farm) from September 2012 to November... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hemoplasmas; Hemotropic mycoplasmas; Dairy herd. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300451 |
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| Faria,Ana Carolina Silva de; Silva,Maria Cristina da; Oliveira Filho,João Xavier; Oliveira,Juçara Tinasi de; Paula,Daphine Ariadne Jesus de; Chitarra,Cristiane Silva; Nakazato,Luciano; Dutra,Valéria. |
| Streptococcus suis é um patógeno que a afeta a produção industrial de suínos em todo o mundo. É de extrema importância, pois está associado a doenças em suínos e humanos. O objetivo deste estudo foi determinar a prevalência do Streptococcus suis tipo 2 em 201 amostras de tonsilas de animais clinicamente sadios a partir da técnica de PCR. As amostras positivas foram submetidas à pesquisa do gene codificador do fator extracelular (ef). Os resultados demonstraram que a prevalência (23,38%) foi maior que em outro estudo recentemente realizado no mesmo Estado, indicando que a PCR é um método mais sensível em relação ao isolamento bacteriano. Houve baixa ocorrência do gene ef* (1,49%), o que mostra uma grande importância para população analisada, pois cepas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptococcus suis tipo 2; PCR; Ef; Suínos. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000100021 |
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