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Efficacy of leptospiral commercial vaccines on the protection against an autochtonous strain recovered in Brazil BJM
Sonada,Rafael Bazaglia; Azevedo,Sérgio Santos de; Soto,Francisco Rafael Martins; Costa,Diego Figueiredo da; Morais,Zenaide Maria de; Souza,Gisele Oliveira de; Gonçales,Amane Paldês; Miraglia,Fabiana; Vasconcellos,Sílvio Arruda.
Abstract In swine and bovines, leptospirosis prevention and control is carried out via vaccination of susceptible animals using bacterins. However, the efficiency of leptospirosis vaccines has been questioned. This work aimed to investigate the potency of five leptospirosis vaccines sold commercially in Brazil, challenging the animals with one autochthonous strain of Leptospira, Canicola serovar, denoted LO4, isolated from swine. The standard protocol was followed, and renal carriers of Leptospira were identified among the surviving animals by culture and PCR. Of the five vaccines tested, only two proved effective. None of the surviving animals was positive by culture; however, one animal was positive by PCR. Three of the five vaccines sold commercially in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospirosis; Vaccination; Production animals; Control.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200347
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Leptospirosis in native mixed-breed sheep slaughtered in a semiarid region of Brazil Ciência Rural
Costa,Diego Figueiredo da; Silva,Aline Ferreira da; Brasil,Arthur Willian de Lima; Loureiro,Ana Paula Pereira; Santos,Fabrine Alexandre dos; Azevedo,Sergio Santos de; Lilenbaum,Walter; Alves,Clebert José.
ABSTRACT: Sheep breeding is an economic activity of great value for the Brazilian livestock industry, and infectious diseases can significantly compromise its productivity. The aim of this study was to detect Leptospira spp. in mixed breed sheep from a semiarid region of the State of Paraíba, Northeast Brazil. In total, 49 blood and renal tissue samples, as well as 25 urine samples, were collected from 49 sheep in the slaughter line. The microscopic agglutination test (MAT) was used to verify the presence of anti -Leptospira spp. antibodies in blood samples. Renal tissue and urine samples were analyzed by both PCR and bacterial culture. Sero reactivity was observed in 8.2% (4/49) of animals. Three renal samples were positive for leptospiral DNA by PCR...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospirosis; Small ruminants; PCR; Serology; Isolation; Slaughterhouse.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000200451
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