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Effect of 0.9% saline solution and phosphate buffer saline at different temperatures and incubation times on the morphology of goat preantral follicles 62
Santos,Regiane Rodrigues dos; Silva,José Roberto Viana; Costa,Sonia Helena Furtado; Rodrigues,Ana Paula Ribeiro; Lôbo,Raimundo Nonato Braga; Figueiredo,José Ricardo de.
The present work investigated the efficiency of 0.9% saline solution and Phosphate Buffered Saline (PBS) in the preservation of goat preantral follicles in situ at different temperatures and incubation times. The ovarian pair of each animal was divided into 19 fragments. One ovarian fragment was taken randomly and fixed (control). The other 18 fragments were randomly distributed in tubes containing 0.9% saline solution or PBS at 4, 20 or 39 ºC for 4, 12 or 24 h. A total of 5,921 preantral follicles were examined. The quality of preantral follicles was evaluated by classical histology. The storage of ovarian fragments in 0.9% saline solution or PBS at 4 ºC did not reduce significantly the percentage of morphologically normal follicles when compared with the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Goat; Preantral follicle; Preservation; 0.9% saline solution; PBS.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000500007
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Preservation of goat preantral follicles in saline or coconut water solution 62
Costa,Sonia Helena Furtado; Santos,Regiane Rodrigues dos; Ferreira,Marcos Antônio Leal; Machado,Vanessa Porto; Rodrigues,Ana Paula Ribeiro; Ohashi,Otávio Mitio; Figueiredo,José Ricardo de.
The present study investigated the efficiency of saline solution and coconut water solution in the preservation of goat preantral follicles enclosed in ovarian tissue, at different temperatures and for different incubation periods. At the slaughterhouse, the ovarian pair was divided into 19 fragments; one ovarian fragment was immediately fixed for histology (control-time zero). The other 18 ovarian fragments were preserved in both solutions at 4ºC, 20ºC or 39ºC for 4 h, 12 h or 24 h. The histological analysis showed that the storage of ovarian fragments in both solutions at 4ºC for up to 24 h kept the percentage of normal preantral follicles similar to the control values. In contrast, preservation at 20°C or 39ºC, in either solution, reduced significantly...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Preservation; Goat; Preantral follicles; Coconut water; Saline solution.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000600009
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