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Registros recuperados: 10
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Alternative genotyping method for the single nucleotide polymorphism A2959G (AF159246) of the bovine CAST gene PAB
Curi,Rogério Abdallah; Chardulo,Luis Artur Loyola; Silveira,Antônio Carlos; Oliveira,Henrique Nunes de.
The objective of this work was to genotype the single nucleotide polymorphism (SNP) A2959G (AF159246) of bovine CAST gene by PCR-RFLP technique, and to report its use for the first time. For this, 147 Bos indicus and Bos taurus x Bos indicus animals were genotyped. The accuracy of the method was confirmed through the direct sequencing of PCR products of nine individuals. The lowest frequency of the meat tenderness favorable allele (A) in Bos indicus was confirmed. The use of PCR-RFLP for the genotyping of the bovine CAST gene SNP was shown to be robust and inexpensive, which will greatly facilitate its analysis by laboratories with basic structure.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Calpastatin gene; Meat texture; PCR-RFLP; SNP.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000500014
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Análise cromossômica e molecular do javali europeu Sus scrofa scrofa e do suíno doméstico Sus scrofa domesticus Braz. J. Vet. Res. Anim. Sci.
Gimenez,Danilo Lucano; Mota,Lígia Souza Lima Silveira da; Curi,Rogério Abdallah; Rosa,Guilherme Jordão de Magalhães; Gimenes,Marcos Aparecido; Lopes,Catalina Romero; Lucca,Edmundo José de.
A presente investigação teve como objetivos: analisar animais presentes em diferentes criações de javalis no estado de São Paulo, com o intuito de auxiliar a identificação de javalis "puros" assim como javalis híbridos provenientes do cruzamento com o suíno doméstico, para tanto foram utilizadas avaliação do fenótipo dos animais, análises citogenéticas e da técnica molecular de RAPD (Random Amplified Polymorphic DNA).O estudo do número de cromossomos nas células diplóides em 104 animais destinados a análise citogenética e fenotípica, revelou polimorfismo de 2n=36, 37 e 38 cromossomos. Por meio da técnica de bandamento GTG foi possível identificação da translocação Robertsoniana entre os cromossomos 15 e 17 como responsável por esse polimorfismo. Todavia,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Javali; Híbrido; Citogenética; RAPD; Fenótipos.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000200009
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Análise genética de características de crescimento e perímetro escrotal em bovinos da raça Brangus PAB
Silva,Josineudson Augusto II de Vasconcelos; Marcelo,Erika Tagima; Ribeiro,Cinthia Brosco; Maiorano,Amanda Marchi; Curi,Rogério Abdallah; Oliveira,Henrique Nunes de; Mota,Marcílio Dias Silveira da.
O objetivo deste trabalho foi estimar os parâmetros genéticos e a tendência genética de características de crescimento e perímetro escrotal em animais da raça Brangus. Dados de 6.340 animais, criados em cinco propriedades nas regiões Sul, Sudeste e Centro‑Oeste do Brasil, foram utilizados para avaliação de: perímetro escrotal (PE) ao sobreano e pesos ao nascer (PN), à desmama (P205), ao ano (P365) e ao sobreano (P550). Os componentes de covariância foram estimados por inferência bayesiana, sob modelo animal, com efeitos fixos de grupos de contemporâneos e de classe de idade da vaca ao parto, e efeitos aleatórios genético aditivo direto e residual. Os efeitos aleatórios genético materno e de ambiente permanente materno foram incluídos somente para PN e...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Herdabilidade; Inferência bayesiana; Valor genético.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000800018
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Bovine carcass sexing by PCR method Braz. J. Vet. Res. Anim. Sci.
Curi,Rogério Abdallah; Mota,Lígia Souza Lima Silveira da; Amarante,Mônica Regina Vendrame; Lopes,Catalina Romeiro.
The objective of the present study was to develop a methodology that would permit sexing bovine meat ready for commercialization. A male-specific primer sequence was used, followed by analysis of the amplified product. The method proved to be efficient for sex verification and is of practical utility in the prevention of fraud in beef sale.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Carcass; Sexing; PCR.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000300007
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Candidate SNPs for carcass and meat traits in Nelore animals and in their crosses with Bos taurus PAB
Curi,Rogério Abdallah; Krauskopf,Monique Marcondes; Hadlich,Janaína Conte; Fortes,Marina Rufino Salinas; Vankan,Dianne Margaret; Silva,Josineudson Augusto II Vasconcelos; Oliveira,Henrique Nunes de; Mota,Marcílio Dias Silveira da.
The objective of this work was to evaluate the effects of single-nucleotide polymorphisms (SNPs) in the genes IGF1 (AF_017143.1:g.198C>T), MSTN (AF_320998.1:g.433C>A), MYOD1 (NC_007313:g.1274A>G) and MYF5 (NC_007303:g.1911A>G) on carcass and meat traits in Nelore (Bos indicus) and Nelore x B. taurus. A total of 300 animals were genotyped and phenotyped for rib eye area (REA), backfat thickness (BT), intramuscular fat (IF), shear force (SF) and myofibrillar fragmentation index (MFI). The effects of allele substitution for each SNP were estimated by regression of the evaluated phenotypes on the number of copies of a particular allele using the general linear model. The polymorphism at IGF1 was non-informative in Nelore animals. In crossbred...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bos indicus; Candidate gene; Fat deposition; Meat tenderness; Polymorphism.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000200019
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Evaluation of nine microsatellite loci and misidentification paternity frequency in a population of Gyr breed bovines Braz. J. Vet. Res. Anim. Sci.
Curi,Rogério Abdallah; Lopes,Catalina Romero.
Paternity misidentification is harmful due to the reduction in annual genetic earnings of the population and because it endangers an efficient genetic improvement program. The objectives of the present study was to evaluate nine microsatellites in Paternity Testing and to investigate misidentification paternity frequency in families of Gyr breed bovines population. In the present experiment blood samples from forty Gir breed families ( bull / cow / calf ), registered pure breed in the Zebu Breeders Brazilian Association (ABCZ) were used. The most part of the microsatellites used in this work were recommended by the International Society of Animal Genetics (ISAG). The genomic DNA extraction was performed from whole blood samples. The microsatellites...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA; Microsatellites; Paternity; Bovine; Gyr.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000300004
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Frequencies of candidate genes and associations with carcass and meat traits in Nellore and crossbred cattle PAB
Dias,Victor Augusto Domingos; Curi,Rogério Abdallah; Pereira,Guilherme Luis; Malheiros,Jéssica Moraes; Espigolan,Rafael; Albuquerque,Lúcia Galvão de; Chardulo,Luis Artur Loyola; Oliveira,Henrique Nunes de.
Abstract: The objective of this work was to estimate allelic frequencies of the polymorphisms IGF2/MboII (G > T) of the insulin-like growth factor 2 (IGF2) gene, DQ499531.1:g.134A > T of the pro-melanin-concentrating hormone (PMCH) gene, and DQ667048.1:g.3290G > T of the RARrelated orphan receptor C (RORC) gene in beef cattle of different genetic groups, and to evaluate the associations between these polymorphisms and traits related to carcass composition and meat quality. Data on carcass and meat quality of 499 animals was used: of 313 Nellore (Bos indicus) and of 186 Nellore crossed with different taurine (Bos taurus) breeds. For the IGF2/MboII polymorphism, the frequencies found for the G allele were 0.231 and 0.631 for Nellore and crossed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bos indicus; Beef cattle; Beef quality; Polymorphism..
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000200169
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Genetic polymorphisms related to meat traits in purebred and crossbred Nelore cattle PAB
Curi,Rogério Abdallah; Fortes,Marina Rufino Salinas; Chardulo,Luis Artur Loyola; Silveira,Antonio Carlos; Arrigoni,Mário De Beni; Martins,Cyntia Ludovico; Assumpção,Mayra Elena Ortiz D' Avila; Oliveira,Henrique Nunes de.
The objective of this work was to estimate the allelic and genotypic frequencies of CAST/XmnI, a calpastatin gene polymorphism, and CAPN530, a calpain 1 large subunit gene polymorphism, in different beef genetic groups (Nelore and Nelore x Bos taurus), and to investigate associations between these polymorphisms and carcass and meat traits. Three hundred animals - comprising 114 Nelore, 67 Angus x Nelore, 44 Rubia Gallega x Nelore, 41 Canchim, 19 Brangus three-way cross and 15 Braunvieh three-way cross- were genotyped by PCR-RFLP and phenotyped for rib-eye area (REA), back-fat thickness (BT), intramuscular fat (IF), shear force (SF) and myofibrillar fragmentation index (MFI). The occurrence of the two alleles of the CAST/XmnI and CAPN530 single nucleotide...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bos indicus; Calpain gene; Calpastatin gene; Meat tenderness; Single nucleotide polymorphisms.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009001200015
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Mitochondrial DNA of Nellore and European x Nellore crossing cattle of high performance PAB
Curi,Rogério Abdallah; Mota,Lígia Souza Lima Silveira da; Silveira,Antônio Carlos.
The objective of this work was to evaluate, through a polymorphism in the ND5 gene of the bovine mitochondrial DNA, the frequency of Bos taurus indicus mtDNA individuals in a sample of Nellore purebred origin animals (n = 69) and crossbred animals originated from crosses of European sires and Nellore purebred origin females (n = 275). Only 2.26% (8/354) of the animals presented Bos taurus indicus mtDNA. The high frequency of Bos taurus taurus mtDNA in these animals can be a consequence of selection, once the animals studied are originated from selected lineages of high performance for meat production.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bos taurus indicus; Bos taurus taurus; Beef cattle; DNA polymorphism.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000800019
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Polymorphisms in candidate genes and their association with carcass traits and meat quality in Nellore cattle PAB
Borges,Bárbara Oliveira; Curi,Rogério Abdallah; Baldi,Fernando; Feitosa,Fabieli Loise Braga; Andrade,Willian Bruno Fernandes de; Albuquerque,Lucia Galvão de; Oliveira,Henrique Nunes de; Chardulo,Luis Artur Loyola.
The objective of this work was to estimate the allele polymorphism frequencies of genes in Nellore cattle and associate them with meat quality and carcass traits. Six hundred males were genotyped for the following polymorphisms: DGAT1 (VNTR with 18 nucleotides at the promoter region); ANK1, a new polymorphism, identified and mapped here at the gene regulatory region NW_001494427.3; TCAP (AY428575.1:g.346G>A); and MYOG (NW_001501985:g.511G>C). In the association study, phenotype data of hot carcass weight, ribeye area, backfat thickness, percentage of intramuscular fat, shear force, myofibrillar fragmentation index, meat color (L*, a*, b*), and cooking losses were used. Allele B from the ANK1 gene was associated with greater redness (a*). Alleles 5R,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bos indicus; Ankyrin; Assisted selection; Beef cattle; Myogenin; Titin.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2014000500364
Registros recuperados: 10
Primeira ... 1 ... Última
 

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