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Registros recuperados: 29
Primeira ... 12 ... Última
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Abnormal meiotic behavior in three species of Crotalaria PAB
Ferreira,Kátia; Torres,Giovana Augusta; Carvalho,Isabelle Vilela de; Davide,Lisete Chamma.
The objective of this work was to compare the meiotic behavior and pollen grain viability of three species of Crotalaria. Slides for meiotic analysis were prepared by the air-drying technique. Pollen grain viability was measured by three staining procedures (Alexander's solution, tetrazolium chloride and fluorescein diacetate) and in vitro germination in a sucrose solution. Eight bivalents were observed, confirming previous reports on populations from other regions of Brazil, as well as from other countries. All species showed abnormal meiotic behavior as follows: in Crotalaria micans, cytomixis and abnormal chromosome pairing in diakinesis; in C. spectabilis, abnormal chromosome pairing in diplotene; in C. zanzibarica, shrunk nuclei in leptotene and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crotalaria; Cytogenetics; Cytomixis; Meiosis; Pollen grain viability.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009001200012
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Application of Comet assay to assess the effects of white bean meal on DNA of human lymphocytes BJPS
Pereira,Luciana Lopes Silva; Marcussi,Silvana; Sátiro,Lívia Cabral; Pereira,Chrystian Araujo; Andrade,Larissa Fonseca; Davide,Lisete Chamma; Santos,Custódio Donizete dos.
This study was conducted to evaluate the potential induction of genotoxic effects of white bean flour using the Comet assay. The test was conducted with human lymphocytes present in whole blood immediately after collection, by incubation with white bean flour in three concentrations (3.92, 9.52 and 18.18 mg/mL) at 37 ºC for 4 h followed by preparation of slides. Samples were considered positive (above 20% damage) when the damage observed to cellular DNA was higher than the negative control. No genotoxic potential was found at the doses tested. However, it would be premature to suggest absence of risk to human health of DNA damage since the exposure of cells to the extract was restricted to four hours rather than a whole cell cycle. Additionally, further...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genotoxicity; White bean flour/genotoxic effects; Comet assay/toxicological analysis of food; Food/toxicological analysis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100012
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Characterization of interphase nuclei from triploid hybrids between Pennisetum purpureum Schumach and Pennisetum glaucum (L.) R. Br. Ciência e Agrotecnologia
Fajardo,Cristiane Gouvêa; Davide,Lisete Chamma; Pereira,Antônio Vander.
This study evaluated the structure and the volume of interphase nuclei from root meristems of the genotypes of napier grass (Pennisetum purpureum), pearl millet (Pennisetum glaucum) and hybrids resultant of such breeding. In napier grass, nuclei were areticulate. Both pearl millet and the triploid hybrid had semi-reticulate nuclei; also, the hybrid presented a small proportion (6%) of areticulate nuclei. Pearl millet had the highest averages of nuclear dimensions, such as volume, diameter and radius, followed by the interspecific hybrid and napier grass. There was no intraspecific variation for the type of nuclear structure, which indicates this feature is important for cytotaxonomic studies involving the genus Pennisetum. Results demonstrated that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Napier grass; Pearl millet; Interphase chromatin; Nuclear volume.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000500007
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Citogenética de Piper hispidinervum e Piper aduncum PAB
Nunes,Juliane Dornellas; Torres,Giovana Augusta; Davide,Lisete Chamma; Salgado,Caio Césio.
O objetivo deste trabalho foi detectar diferenças entre duas espécies de pimenta-longa por meio de análise de cariótipos. Foram analisados cinco acessos de Piper hispidinervum (Piperaceae) C. DC. e Piper aduncum L. (Piperaceae) pertencentes à coleção de germoplasma da Embrapa Acre, utilizando-se o método de esmagamento e coloração de Feulgen. As duas espécies apresentaram número cromossômico 2n = 24 e cromossomos pequenos e metacêntricos com comprimento médio de 1,38 µm em P. hispidinervum e 1,32 µm em P. aduncum. Pelos descritores citogenéticos obtidos não há diferença entre as duas espécies.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citologia; Diferença biológica; Cariótipo; Piperaceae; Número de cromossomos.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000700019
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Colchicine and oryzalin effects on tetraploid induction and leaf anatomy of Solanum commersonii ssp. Ciência Rural
Tomé,Lívia Gracielle Oliveira; Silva,Adriano Bortolotti da; Pinto,Cesar Augusto Brasil Pereira; Davide,Lisete Chamma; Pereira,Dalvana Sousa; Carvalho,Carlos Roberto de.
ABSTRACT: The aim of the present study was to evaluate the effect of the colchicine and oryzalin anti-mitotic substances on the induction of tetraploid plants and foliar anatomy of two diploids clones of Solanum commersonii ssp. Nodal segments of Solanum commersonii subsp. commersonii Dun. and Solanum commersonii subsp. malmeanum Bitt. were treated with colchicine (3.5, 5.0, and 6.5mM; 72h) or oryzalin (10, 30, and 50µM; 24h). After the treatment with anti-mitotic substances, nodal segments were inoculated in the MS culture medium and cultivated in vitro (60 days). After in vitro cultivation, the plants were transferred to vases with the substrate Plantmax(r) and kept in the greenhouse (45 days). Plant ploidy level was assessed by flow cytometry and leaf...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Potato; Crop breeding; Flow citometry; Foliar anatomy.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001101973
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Coloração de núcleos de esporos e hifas do cogumelo Agaricus blazei Ciência e Agrotecnologia
Labory,Cláudia Regina Gontijo; Dias,Eustáquio Souza; Davide,Lisete Chamma; Schawan,Rosane Freitas; Wenzel,Inajá Marchizeli.
Com o objetivo de se estudar o comportamento nuclear durante o ciclo sexual do cogumelo Agaricus blazei , técnicas de coloração e a utilização de fluorocromos foram testadas, visando a uma padronização metodológica para estudos citogenéticos futuros. Das técnicas avaliadas neste trabalho (método de Feulgen, coloração com Giemsa e fluorescência-DAPI), a coloração com Giemsa e o fluorocromo DAPI apresentaram os melhores resultados para evidenciar núcleos. O corante Giemsa permitiu a visualização de compartimentos multinucleados. Com o DAPI, não foi possível a visualização dos septos, sendo necessária a utilização adicional de calcofluor, que tem a propriedade de corar a quitina que delimita os compartimentos das hifas.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agaricus blazei; Giemsa; DAPI; Calcofluor; Citogenética.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000200030
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Cytogenetics of Pennisetum purpureum Schumack x Pennisetum glaucum L. hybrids and their parents Ciência e Agrotecnologia
Barbosa,Sandro; Davide,Lisete Chamma; Pereira,Antônio Vander.
Cytogenetic studies were carried out on five accesses of interspecific hybrids of Pennisetum purpureum and Pennisetum glaucum and their parentals, which are part of the Forage Germplasm Bank of the "Centro Nacional de Pesquisa de Gado de Leite" of EMBRAPA, Brazil. The mitotic cytogenetic analysis, performed using stain by Schiff reactive, confirmed the chromosome number of 2n = 14, 28 and 21 for the accesses of P. glaucum, P. purpureum and their hybrids, respectively. For each access, descriptions of the chromosome complement and idiograms are presented which allowed to establish the karyotypic relationships between the hybrids and their parentals. Based on the total chromosomal length data it was possible to establish the karyotype classification of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Elephant grass; Pearl millet; Interspecific hybrids; Cytogenetic; Ideograms; Chromosome.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000100003
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Cytotaxonomy of some species and of interspecific hybrids of Pennisetum (Poaceae, Poales) Genet. Mol. Biol.
Techio,Vânia Helena; Davide,Lisete Chamma; Pereira,Antônio Vander; Bearzoti,Eduardo.
Cytotaxonomic studies were carried out on 26 accessions of Pennisetum spp. originating from the Active Germplasm Bank of Embrapa Gado de Leite-Juiz de Fora, Minas Gerais, Brazil.This study presents cytogenetics and reproductive morphological data for each one of these accessions, which allowed groupings and suggest botanical identifications to be established. The metaphases of the accessions characterized as P. purpureum confirmed the presence of 2n = 28 chromosomes that have already been described for this species. For the hybrids between P. purpureum and P. glaucum and for the hexaploids, resulting from the chromosome duplication of these hybrids, 2n = 21 and 2n = 42 chromosomes were confirmed, respectively. The wild accession identified as P. setosum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pennisetum; Cytogenetic; Taxonomy; Germplasm; Genetic divergence.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200014
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Duplicação cromossômica de gramíneas forrageiras: uma alternativa para programas de melhoramento genético Ciência Rural
Pereira,Roselaine Cristina; Davide,Lisete Chamma; Techio,Vânia Helena; Timbó,Ana Luiza Oliveira.
A obtenção artificial de genótipos com duplicação cromossômica em forrageiras busca maximizar características de interesse agronômico, como valor nutricional e produção de forragem, resistência a pragas e doenças, tolerância a estresses abióticos e restauração da fertilidade de híbridos estéreis. Outro objetivo da duplicação cromossômica é a geração de variabilidade genética em espécies apomíticas, por meio da duplicação cromossômica de acessos sexuais igualando a ploidia de modo a permitir a realização de cruzamentos e a obtenção de descendentes férteis. A indução de poliploidia é feita utilizando substâncias antimitóticas, sendo a colchicina a mais amplamente usada em plantas forrageiras. Entretanto, devido a sua toxicidade, outras substâncias como...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Poliploidização; Substâncias antimitóticas; Colchicina; Herbicidas; Cafeína; Explantes.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000700023
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Effects of sorghum (sorghum bicolor L.) root exudates on the cell cycle of the bean plant (phaseolus vulgaris L.) root Genet. Mol. Biol.
Hallak,Angela Maria Gattás; Davide,Lisete Chamma; Souza,Itamar Ferreira.
Two experiments were conducted to test the allelopathic effect of sorghum (Sorghum bicolor L.) root exudates on bean (Phaseolus vulgaris L.) cell division. Research was conducted in the greenhouse of the Wistock Agricultural Research Institute of Minas Gerais State (EPAMIG) and in a laboratory of the Federal University of Lavras (UFLA). Sorghum variety BR-601 and bean variety Carioca MG were used. The exudate, called sorgoleone (SGL), was obtained by methylene chloride and acetic acid extraction from sorghum roots seven days after sowing on Petri dishes, and refrigerated until use. Solutions of 0.01, 0.05, 0.10, and 0.15 mM were prepared using Johanson solution as the SGL solvent. Seven-day-old bean seedlings grown in vermiculite in a greenhouse were...
Tipo: Info:eu-repo/semantics/article
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100018
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Embriogênese somática em híbridos de Pennisetum sp. e avaliação de estabilidade genômica por citometria PAB
Campos,José Marcello Salabert de; Calderano,Clara de Azevedo; Pereira,Antônio Vander; Davide,Lisete Chamma; Viccini,Lyderson Facio; Santos,Marcelo de Oliveira.
Os objetivos deste trabalho foram estabelecer um protocolo eficiente de embriogênese somática, em híbridos triploides entre capim elefante (Pennisetum purpureum Schumach.) e milheto (P. glaucum (L.) R. Br.), e avaliar por citometria de fluxo a estabilidade genômica das plantas obtidas in vitro. A embriogênese somática e a regeneração das plantas foram estabelecidas a partir de embriões zigóticos maduros de híbridos entre capim elefante e milheto. Foram testados quatro tratamentos com 2,4 ácido diclorofenoxiacético (2,4 D), nas concentrações 0, 1, 2 e 3 mg L-1, para indução de calos embriogênicos, e dois tratamentos com inositol a 1 e 2 g L-1, para regeneração das plantas. Os tratamentos foram dispostos em delineamento inteiramente ao acaso. A combinação...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pennisetum glaucum; Pennisetum purpureum; Cultura de tecidos.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000100006
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Época da indução e evocação floral em Citrus sinensis (L.) Osbeck cv. Pêra Rio Ciência Rural
Pereira,Iara Alvarenga Mesquita; Pinto,José Eduardo Brasil Pereira; Davide,Lisete Chamma.
A produção do estímulo floral (indução) e emissão das primeiras sépalas, na gema floralmente determinada (evocação floral) em Citrus sinensis (L.) Osbeck cv. Pêra Rio pôde ser acompanhada pelas variações anatômicas nos meristemas apicais e axilares. Com objetivo de determinar a época na qual ocorre a indução e evocação floral, cortes longitudinais de gemas apicais e axilares, corados com pironina Y-methylgreen foram efetuados em períodos regulares de maio a agosto. Através das mudanças no formato da gema, principalmente diâmetro, que aumentou com a indução, foi possível determinar a época do ano na qual gemas vegetativas são induzidas a florescerem. Foram detectadas variações nos diâmetros das gemas no período de início da morfogênese floral (meados até o...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Laranja; Citrus sinensis; Rutaceae; Florescimento; Indução floral; Evocação floral.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000500010
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Forage potential of Urochloa genotypes by using leaf anatomy Ciência Rural
Mauri,Janaína; Pereira,Daniele Laís; Silva,Gabrielle Avelar; Davide,Lisete Chamma; Techio,Vânia Helena; Souza Sobrinho,Fausto; Pereira,Fabricio José.
ABSTRACT: The digestibility potential of leaves from forages depends on the amount of nutrition in their tissues, with low lignin deposition in the cell wall, mainly parenchyma and phloem. This research evaluated the leaf structure of different Urochloa genotypes and discussed its potential for evaluating digestibility. The cultivars U. brizantha, U. decumbens, U. ruziziensis and three clones of U. ruziziensis (1, 95 and 97), which are under development in breeding programs, were evaluated. Plants were grown under the recommended culture conditions for the Urochloa species. Plants were cut 60 days after sowing, and leaves were collected at 15 days of regrowth. Leaves were fixed in FAA 70 and further stored in 70% ethanol until being submitted to the usual...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Digestibility; Forage production; Leaf anatomy; Sclerenchyma; Lignin.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000400401
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Genomic behavior of hybrid combinations between elephant grass and pearl millet PAB
Leão,Fernando Ferreira; Davide,Lisete Chamma; Campos,José Marcello Salabert de; Pereira,Antonio Vander; Bustamante,Fernanda de Oliveira.
The objective of this work was to evaluate the genomic behavior of hybrid combinations between elephant grass (Pennisetum purpureum) and pearl millet (P. glaucum). Tetraploid (AAA'B) and pentaploid (AA'A'BB) chromosome races resulting from the backcross of the hexaploid hybrid to its parents elephant grass (A'A'BB) and pearl millet (AA) were analyzed as to chromosome number and DNA content. Genotypes of elephant grass, millet, and triploid and hexaploid induced hybrids were compared. Pentaploid and tetraploid genomic combinations showed high level of mixoploidy, in discordance with the expected somatic chromosome set. The pentaploid chromosome number ranged from 20 to 34, and the tetraploid chromosome number from 16 to 28. Chromosome number variation was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pennisetum glaucum; Pennisetum purpureum; DNA content; Interspecific hybrids; Mixoploidy.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000700006
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Histogram score contributes for reliability of DNA content estimatives in Brachiaria spp Ciência e Agrotecnologia
Timbó,Ana Luiza de Oliveira; Pereira,Roselaine Cristina; Santos,Vanderley Borges dos; Souza Sobrinho,Fausto; Davide,Lisete Chamma.
Flow cytometry allows to estimate the DNA content of a large number of plants quickly. However, inadequate protocols can compromise the reliability of these estimates leading to variations in the values of DNA content the same species. The objective of this study was to propose an efficient protocol to estimate the DNA content of Brachiaria spp. genotypes with different ploidy levels using flow cytometry. We evaluated four genotypes (B. ruziziensis diploid and artificially tetraploidized; a tetraploid B. brizantha and a natural triploid hybrid), three buffer solutions (MgSO4, Galbraith and Tris-HCl) and three species as internal reference standards (Raphanus sativus, Solanum lycopersicum e Pisum sativum). The variables measured were: histogram score (1-5),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brachiaria ruziziensis; Brachiaria brizantha; Colchicine; Nuclear DNA content.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000600001
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Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids PAB
Santos,Fernanda Motta da Costa; Torres,Giovana Augusta; Techio,Vânia Helena; Pereira,Antônio Vander; Davide,Lisete Chamma.
Abstract: The objective of this work was to evaluate, by genomic in situ hybridization (GISH), pairing configurations as potential indicators of recombination between chromosomes of different parental genomes, in two interspecific hybrids (elephant grass x pearl millet) artificially polyploidized. Anthers from young flower buds were used in the chromosomal preparations. The genomic probe was prepared with pearl millet DNA and labeled with biotin-16-dUTP by the nick translation reaction. Blocking DNA was prepared with genomic elephant grass DNA. The homoeologous intergenomic pairing, observed in the two hybrids, indicates the possibility of recombination between chromosomes of the parental genomes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pennisetum glaucum; Pennisetum purpureum; In situ hybridization; Intergenomic pairing; Interspecific hybridization.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000900814
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Karyotipic asymmetry of both wild and cultivated species of Pennisetum Bragantia
Techio,Vânia Helena; Davide,Lisete Chamma; Cagliari,Alexandro; Barbosa,Sandro; Pereira,Antônio Vander.
This study aimed the establishment of the relation between karyotipic asymmetry values obtained for different accessions of both wild and cultivated species of Pennisetum from Germplasm Bank of Embrapa Gado de Leite/Juiz de Fora-Minas Gerais State, Brazil. Conventional cell cycle synchronization protocols and Feulgen staining method were used to obtain metaphases plates. The wild-type accessions corresponded to the species P. setosum (2n=6x=54), P. nervosum (2n=4x=36), and P. orientale (2n=4x=36), and the cultivated to P. purpureum (2n=4x=28) and P. glaucum (2n=2x=14). No significant difference was found for the total length of chromosomes (p>0.05) among the species. The analysis of intra-chromosomal asymmetry (A1) and inter-chromosomal asymmetry (A2)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pennisetum purpureum; Pennisetum glaucum; Germplasm; Karyotype.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052010000200003
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KARYOTYPE ALTERATIONS AFTER HYBRIDIZATION BETWEEN Pennisetum purpureum AND Pennisetum glaucum Ciência e Agrotecnologia
Reis,Gabriela Barreto dos; Andrade-Vieira,Larissa Fonseca; Campos,José Marcello Salabert de; Pereira,Antonio Vander; Davide,Lisete Chamma.
ABSTRACTNapier grass and pearl millet are tropical forages from the genus Pennisetum. The variability in those species is explored in breeding programs of forages, as well as in the production of interspecific hybrids. Hybridization is a phenomenon that leads to intergenomic conflicts following the elimination of genomic sequences. In this sense, the present work aimed to study the genomic alterations occurring after interspecific hybridization of pearl millet and Napier grass with the use of cytogenetics and flow cytometry tools. These methods allowed the evaluation of chromosome morphometry, DNA content and genomic ratio in pearl millet, Napier grass and hybrids. It was observed that pearl millet and Napier grass have chromosomes with superposed size....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Interspecific hybrids; Chromosome morphology; DNA content; In situhybridization.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542015000500443
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Meiosis in elephant grass (Pennisetum purpureum), pearl millet (Pennisetum glaucum) (Poaceae, Poales) and their interspecific hybrids Genet. Mol. Biol.
Techio,Vânia Helena; Davide,Lisete Chamma; Pereira,Antônio Vander.
The cultivated and sexually compatible species Pennisetum purpureum (elephant grass, 2n = 4x = 28) and Pennisetum glaucum (pearl millet, 2n = 2x = 14) can undergo hybridization which favors the amplification of their genetic background and the introgression of favorable alleles into breeding programs. The main problem with interspecific hybrids of these species is infertility due to triploidy (2n = 3x = 21). This study describes meiosis in elephant grass x pearl millet hybrids and their progenitors. Panicles were prepared according to the conventional protocol for meiotic studies and Alexander’s stain was used for assessing pollen viability. Pearl millet accessions presented regular meiosis with seven bivalents and high pollen viability. For elephant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Meiosis; Pennisetum; Elephant grass; Interspecific hybrids; Pearl millet; Pollen.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200025
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Mitodepressive and clastogenic effects of aqueous extracts of the lichens Myelochroa lindmanii and Canoparmelia texana (Lecanorales, Parmeliaceae) on meristematic cells in plant bioassays Genet. Mol. Biol.
Campos,José Marcello Salabert de; Davide,Lisete Chamma; Soares,Geraldo Luiz Gonçalves; Viccini,Lyderson Facio.
The cytotoxicity effect of aqueous extracts of the lichens species Myelochroa lindmanii and Canoparmelia texana (Lecanorales, Parmeliaceae) were evaluated using meristematic cells of lettuce (Lactuca sativa) and maize (Zea mays). The seasonal effect was also evaluated. Extracts of M. lindmanii and C. texana inhibited root growth and/or percentage germination, possibly due to alterations in the cell cycle. The M. lindmanii extract showed anti-mitotic effects and blocked the cell cycle in metaphase so that c-mitosis and cells with chromosome duplication were produced. The C. texana extract appeared to hinder cell division, increasing the number of interphase cells. In addition, both extracts caused an increase in percentage of cell death. Clastogenic effects...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Canoparmelia texana; Cytotoxicity; Mitotic index; Myelochroa lindmanii; Plant bioassays.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100024
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