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Biochemical and morphological analysis of cell death induced by Egyptian cobra (Naja haje) venom on cultured cells J. Venom. Anim. Toxins incl. Trop. Dis.
Omran,M. A. A.; Fabb,S. A.; Dickson,G..
We investigated the in vitro process of cell death caused by Egyptian cobra venom on primary human embryonic kidney (293T) and mouse myoblast (C2C12) cell lines. The aim of these studies was to provide further information about triggering cell death, and suggest methods for eliminating unwanted cells, such as tumour cells. Both cell lines were treated with 10, 20, and 50 m g/ml of Egyptian cobra (Naja haje) venom in serum free media (SFM) and incubated for 8 hours. Total activities of the lactate dehydrogenase (LDH) and creatine kinase (CK) released in the culture during venom incubation were used as an indicator of the venom in vitro cytotoxicity. Cell injury was morphologically recognized and apoptosis determined by a Fluorescing Apoptosis Detection...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cobra venom; Cytotoxicity; Apoptosis.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300004
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