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Activation of the alternative complement pathway in canine normal serum by Paracoccidioides brasiliensis BJM
Bianchini,A.A.C.; Petroni,T.F.; Fedatto,P.F.; Bianchini,R.R.; Venancio,E.J.; Itano,E.N.; Ono,M.A..
The dimorphic fungus Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, a human granulomatous disease. Recently the first case of natural disease in dogs was reported. The complement system is an important effector component of humoral immunity against infectious agents. Therefore, the aim of this study was to evaluate the activation of the dog alternative complement pathway by P. brasiliensis. Initially, the ability of erythrocytes of guinea pig, rabbit, sheep, chicken and swine to activate the dog alternative pathway was evaluated. The guinea pig erythrocytes showed the greatest capacity to activate dog alternative pathway. The alternative (AH50) hemolytic activity was evaluated in 27 serum samples from healthy dogs and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Paracoccidioidomycosis; Dog; Complement.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200005
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Leukotrienes are not essential for the efficacy of a heterologous vaccine against Mycobacterium tuberculosis infection BJMBR
Franco,L.H.; Paula,M. Oliveira e; Wowk,P.F.; Fonseca,D.M. da; Sérgio,C.A.; Fedatto,P.F.; Gembre,A.F.; Ramos,S.G.; Silva,C.L.; Medeiros,A.I.; Faccioli,L.H.; Bonato,V.L.D..
Leukotrienes are reported to be potent proinflammatory mediators that play a role in the development of several inflammatory diseases such as asthma, rheumatoid arthritis and periodontal disease. Leukotrienes have also been associated with protection against infectious diseases. However, the role of leukotrienes in Mycobacterium tuberculosis infection is not understood. To answer this question, we studied the role of leukotrienes in the protective immune response conferred by prime-boost heterologous immunization against tuberculosis. We immunized BALB/c mice (4-11/group) with subcutaneous BCG vaccine (1 x 10(5) M. bovis BCG) (prime) followed by intramuscular DNA-HSP65 vaccine (100 µg) (boost). During the 30 days following the challenge, the animals were...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Tuberculosis; Prime-boost immunization; Leukotrienes.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700006
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