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The alga Bryothamnion seaforthii contains carbohydrates with antinociceptive activity BJMBR
Vieira,L.A.P.; Freitas,A.L.P.; Feitosa,J.P.A.; Silva,D.C.; Viana,G.S.B..
Bryothamnion seaforthii, a red alga common to the Northeastern coast of Brazil, was used to prepare the protein fraction F0/60 by ammonium sulfate precipitation. The chromatography of F0/60 on DEAE-Sephadel column resulted in two lectin fractions, PI and PII, which have antinociceptive properties in rodents. We determined the antinociceptive activity of the PII fraction and of a carbohydrate-containing fraction (CF) in mice. The CF was prepared from the dried algae, after digestion with 100 mM sodium acetate, pH 6.0, containing 5 mM cysteine, EDTA and 0.4% papain, at 60ºC. A 10% cetylpyridinium chloride was added to the filtrate, and the precipitate was dissolved with 2 M NaCl:ethanol (100:15, v/v) followed by the carbohydrate precipitation with ethanol....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Algae; Antinociceptive activity; Carbohydrate; Bryothamnion seaforthii.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700017
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