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Long-term maintenance of in vitro cultures affects growth and secondary metabolism of St. John's Wort Ciência Rural
Figueiró,Adriana de Andrade; Correa,Cynthia Manira; Astarita,Leandro Vieira; Santarém,Eliane Romanato.
Hypericum perforatum is a traditional medicinal plant with wound healing and antidepressant properties. Efficiency of micropropagation is often related to the long term maintenance of tissues in culture, which may alter the secondary metabolism of plants. The objective of this study was to evaluate growth and secondary metabolism of in vitro shoots of H. perforatum on short and long term maintenance of cultures (30 and 100 days). The effect of BA and NAA supplementation was evaluated during 30 days of culture. Adventitious shoots were cultivated on MS medium supplemented with 4.4mM BA alone or in combination with 0.05mM NAA for 30 days. A hormone-free medium was used as control. Shoots cultivated for 100 days were maintained in presence of 4.4mM BA....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tissue culture; Hypericum perforatum; Hypericin; Phenolic compounds.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001000010
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