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Assessment of silver-stained AFLP markers for studying DNA polymorphism in proso millet (Panicum miliaceum L.) Rev. Bras. Bot.
Karam,Décio; Westra,Philip; Niessen,Scott J.; Ward,Sarah M.; Figueiredo,José Edson Fontes.
Proso millet (Panicum miliaceum L.) is a serious weed in North America. A high number of wild proso millet biotypes are known but the genetic basis of its phenotypic variation is poorly understood. In the present study, a non-radioactive silver staining method for PCR-Amplified Fragment Length Polymorphism (AFLP) was evaluated for studying genetic polymorphism in American proso millet biotypes. Twelve biotypes and eight primer combinations with two/three and three/three selective nucleotides were used. Pair of primers with two/three selective nucleotides produced the highest number of amplified DNA fragments, while pair of primers with three/three selective nucleotides were more effective for revealing more polymorphic DNA fragments. The two better primer...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AFLP; Genetic diversity; Molecular markers; Proso-millet.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042006000400011
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Molecular analysis of endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.) BJM
Figueiredo,José Edson Fontes; Gomes,Eliane Aparecida; Guimarães,Claudia Teixeira; Lana,Ubiraci Gomes de Paula; Teixeira,Marta Aparecida; Lima,Guilherme Vitor Corrêa; Bressan,Wellington.
Endophytic bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and abiotic stresses. In the present study molecular methods were used for identifying Bacillus endophytic bacteria isolated from Brazilian sweet corn. SDS-PAGE of wholecell protein extract of fortytwo isolates revealed a high number of scrutinable bands. Twenty-four isolates were identified in nine different groups of duplicated bacteria and eighteen were identified as unique. Some high-accumulated polipeptides with variable length were observed in almost isolates. Partial sequencing of 16S ribosomal gene revealed that all isolates are Bacillus sp. and among thirteen isolates with similar protein profiles, two were different strains....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic bacteria; Bacillus; Sweet corn; SDS-PAGE; RDNA sequencing.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300014
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Novel insights into the early stages of infection by oval conidia of Colletotrichum sublineolum Scientia Agricola
Souza-Paccola,Ednéia Aparecida de; Bomfeti,Cleide Aparecida; Tanaka,Francisco André Ossamu; Massola Junior,Nelson Sidnei; Colauto,Nelson Barros; Figueiredo,José Edson Fontes; Paccola-Meirelles,Luzia Doretto.
Anthracnose, caused by Colletotrichum sublineolum Henn. ex Sacc. & Trotter, is one of the most important sorghum [Sorghum bicolor (L.) Moench] diseases in Brazil. This fungus exhibits conidial dimorphism, producing either falcate or oval conidia on solid and liquid media, respectively. We compared patterns of the initial infection events by these two types of conidia on sorghum leaves using light microscopy and scanning electron microscopy. The infection events during the first 24 h were similar for both oval and falcate conidia. Globose and melanized apressoria were formed at 24 h after inoculation (hai) regardless of the conidia type. Dense mycelium and oval conidia developed from germinated falcate conidia at 32 hai. Hyphal mass displaying acervuli...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sorghum bicolor; Anthracnose; Conidial dimorphism; Infection biology; Secondary sporulation.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000400351
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Yield losses in off-season corn crop due to stalk rot disease PAB
Costa,Rodrigo Véras da; Simon,Jones; Cota,Luciano Viana; Silva,Dagma Dionísia da; Almeida,Rodrigo Estevam Munhoz de; Lanza,Fabrício Eustáquio; Lago,Bruno Cocco; Pereira,Alan Alves; Campos,Leonardo José Motta; Figueiredo,José Edson Fontes.
Abstract: The objective of this work was to assess yield losses due to stalk rot in corn (Zea mays) hybrids, as well as to identify the main pathogenic fungi responsible for the disease during the off-season, in the state of Tocantins, Brazil. Two field experiments were carried out, one with five hybrids, in 2015, and another with four, in 2016. Ears of healthy and stalk rot-infected plants were collected from each plot, at harvest. Ear size, grain and ear weights, and grain moisture content were measured. From each diseased plant, a stalk piece with two to three nodes was removed for pathogen identification and quantification. The most common pathogens were: Fusarium graminearum, Stenocarpella maydis, and Macrophomina phaseolina. All yield parameters were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fusarium graminearum; Zea mays; Lodging; Stalk breakage; Stem disease.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102300
Registros recuperados: 4
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