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Registros recuperados: 8
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Challenges of microsatellite isolation in fungi Inra
Dutech, C.; Enjalbert, J.; Fournier, E.; Delmotte, F.; Barrès, B.; Carlier, J.; Tharreau, D.; Giraud, T..
Although they represent powerful genetic markers in many fields of biology, microsatellites have been isolated in few fungal species. The aim of this study was to assess whether obtaining microsatellite markers with an acceptable level of polymorphism is generally harder from fungi than in other organisms. We therefore surveyed the number,nature and polymorphism level of published microsatellite markers in fungi from the literature and from our own data on seventeen fungal microsatellite-enriched libraries, and in five other phylogroups (angiosperms, insects, fishes, birds and mammals). Fungal microsatellites indeed appeared both harder to isolate and to exhibit lower polymorphism than in other organisms. This appeared to be due, at least in part, to...
Tipo: Journal Article Palavras-chave: MARQUEUR GENETIQUE; MICROSATELLITE; CHAMPIGNON; ANALYSE DE DONNEES SSR; PLANT PATHOGEN; POLYMORPHIC; MARKERS; DEVELOPMENT; ISOLATION; FEATURES; AFFECTING POLYMORPHISM.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200780b37b09&uri=/notices/prodinra1/2008/04/
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Effect of host plants on sympatric genetic differentiations in french populations of Botrytis cinerea. Abstract Inra
Fournier, E.; Confais, J.; Decognet, V.; Fermaud, M.; Bout, A.; Walker, A.S..
Tipo: Conference Paper Palavras-chave: MICROSATELLITE; MARQUEUR GENETIQUE VINEYARD; BRAMBLES.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008d3e99ce2&uri=/notices/prodinra1/2008/06/
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GEMO: an innovative project on Evolutionary Genomics of Magnaporthe oryzae Inra
Fournier, E.; Tharreau, D.; Kroj, T.; Chiapello, H.; Aguileta, G.; Rodolphe, F.; Gendrault, A.; Lebrun, M.H..
Developing integrated control methods against pests of cultivated plants can significantlycontribute to increasing food production while reducing inputs threatening the environment.The durability of a control method can be improved by a better knowledge of the pathogen’sgenetic determinants that are responsible for this adaptation. We were granted by the FrenchNational Research Agency for a project that aims at sequencing the genomes of several strainsof the phytopathogenic model species Magnaporthe oryzae and at exploiting these completesequences to characterize the repertoire of genes involved in pathogenicity and hostspecificity, and study their evolution. We will sequence 7 strains of the species M. oryzaerepresenting different genetic groups...
Tipo: Conference Paper Palavras-chave: MAGNAPORTHE; GENOME; EFFECTEUR; SEQUENÇAGE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010f5f5626f&uri=/notices/prodinra1/2010/08/
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Identification and functional analysis of Magnaporthe oryzae effectors Inra
Ribot, C.; Cesari, S.; Hirsch, J.; Vincent, D.; Gagey, M.J.; Vallet, J.; Gautier, P.; Notteghem, J.L.; Job, C.; Job, D.; Fournier, E.; Plomion, C.; Morel, J.B.; Lebrun, M.H.; Kroj, T..
The ascomycete Magnaporthe oryzae causes « blast disease » affecting several cereals inparticular rice. During the compatible interaction, the first phase of M. oryzae infection cycleis biotrophic. M. oryzae is suspected to secrete, as bacterial and oomycete pathogens, anarsenal of effector proteins that disrupt the activation and execution of plant defenses. In orderto identify M. oryzae effectors, a search for putatively secreted proteins during rice infectionwas performed from different sequence datasets such as, genome annotation data,transcriptome data of blast fungus-infected rice leaves, and proteomic data of blast proteinsreleased in culture media. A list of 370 candidate effectors expressed during rice infection andpossessing a putative signal...
Tipo: Conference Paper Palavras-chave: EFFECTEUR; CHAMPIGNON; MAGNAPORTHE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20107b2d38db&uri=/notices/prodinra1/2010/08/
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La reproduction sexuée de Magnaporthe oryzae existe-t-elle au champ ? Inra
Saleh, D.; Fournier, E.; Tharreau, D..
Magnaporthe oryzae est un champignon pathogène du riz et d’autres Poacées. Chez cetAscomycète, la reproduction sexuée n’a jamais été observée dans la nature mais certainessouches gardent la capacité de se croiser en conditions contrôlées. M. oryzae esthétérothallique, la reproduction sexuée nécessite donc 2 souches de signes de compatibilitésexuelle (SCS) opposés (Mat1.1 et Mat1.2 codés par 2 gènes différents au locus MAT). Deplus, au moins une des 2 souches doit être femelle-fertile, c’est à dire capable de différencierles périthèces, structures femelles où se déroule la méiose. La structure génétique despopulations collectées sur riz suggère un mode de reproduction majoritairement asexué danspresque toute l’aire de répartition. Mais l’existence de...
Tipo: Conference Paper Palavras-chave: REPRODUCTION; MAGNAPORTHE; TYPE SEXUEL.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010499d477b&uri=/notices/prodinra1/2010/08/
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Microsatellite loci to recognize species for the cheese starter and contaminating strains associated with cheese manufacturing Inra
Giraud, F.; Giraud, T.; Aguileta, G.; Fournier, E.; Samson, R.; Cruaud, C.; Lacoste, S.; Ropars, J.; Tellier, A.; Dupont, J..
We report the development of 17 microsatellite markers in the cheese fungi Penicillium camemberti and P. roqueforti, using an enrichment protocol. Polymorphism and cross-amplification were explored using 23 isolates of P. camemberti, 26 isolates of P. roqueforti and 2 isolates of each of the A chtysogenum and P. nalgiovense species. used to produce meat fermented products. The markers appeared useful for differentiating species, both using their amplification sizes and the sequences of their flanking regions. The microsatellite locus PC4 was particularly suitable for distinguishing contaminant species closely related to P. camemberti and for clarifying the phylogenetic relationship of this species with its supposed ancestral form, P. commune. We analyzed...
Tipo: Journal Article Palavras-chave: CONTAMINANT; DOMESTICATION; PC4 MARKER; MULTIGENIC PHYLOGENY; PENICILLIUM; STARTER CULTURES.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20112cbb5aae&uri=/notices/prodinra1/2011/01/
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Un cadre théorique pour étudier les interactions plantes - champignons pathogènes foliaires Inra
Andanson, A.; Fournier, E.; Grognard, F.; Halkett, F.; Mailleret, L.; Tharreau, D.; Ravigné, V..
Les organismes vivant tirent leurs ressources de l’environnement et les allouent auxdifférentes fonctions biologiques assurant leur développement (croissance, survie,reproduction…). Or les ressources disponibles dans un environnement donné sont finies etmême la plupart du temps limitantes. L’individu doit donc faire des compromis dansl’allocation des ressources à ses différentes fonctions biologiques. Ces compromis vont seretrouver au niveau des traits d’histoire de vie (âge et taille à maturité, nombre dedescendants…), conditionnant ainsi les capacités d’adaptation des individus à leurenvironnement. Chez les champignons phytopathogènes, on peut distinguer deux modesd’exploitation des ressources contrastés : la nécrotrophie, où le pathogène exploite des...
Tipo: Conference Paper Palavras-chave: STRATEGIE OPTIMALE D’ALLOCATION DES RESSOURCES; COMPROMIS; BIOTROPHE; NECROTROPHE; TRAITS D’HISTOIRE DE VIE; AGRESSIVITE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201017e589aa&uri=/notices/prodinra1/2010/08/
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Variabilité et déterminisme génétique des traits de vie impliqués dans l’évolution de l’agressivité et l’adaptation à la résistance partielle Inra
Fournier, E.; Mississipi, S.; Milazzo, J.; Tertois, C.; Ravigné, V.; Tharreau, D..
La recherche des traits impliqués dans la virulence et adaptatifs pour un hôte ou son parasite,l’étude des compromis entre ces traits et des bases génétiques de ces traits, sont cruciales pourla compréhension de l’évolution de la virulence. Dans les pathosystèmes cultivés, la virulence(classiquement définie comme la baisse de fitness infligée à l’hôte par son parasite) estremplacée par la notion de pouvoir pathogène défini comme la quantité de dommages infligéeà l’hôte. Une partie du pouvoir pathogène dépend d’interactions spécifiques qualitatives detype gène pour gène prenant de facto en compte la variabilité des génotypes hôtes et parasites.Par contre, très peu de travaux intégrant les interactions génotype X génotype (G X G) ontporté sur l’agressivité,...
Tipo: Conference Paper Palavras-chave: ADAPTATION; RESISTANCES PARTIELLES; POUVOIR PATHOGENE; TRAITS DE VIE; MAGNAPORTHE ORYZAE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20102c50cb4e&uri=/notices/prodinra1/2010/08/
Registros recuperados: 8
Primeira ... 1 ... Última
 

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