Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: 

RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 10
Primeira ... 1 ... Última
Imagem não selecionada

Imprime registro no formato completo
Biochemical and catalytic properties of three recombinant alcohol acyltransferases of melon. Sulfur-containing ester formation, regulatory role of CoA-SH in activity, and sequence elements conferring substrate preference Inra
Lucchetta, L.; Manriquez-Becerra, D.; El-Sharkawy, I.; Flores, F.B.; Sanchez-Bel, P.; Zouine, M.; Ginies, C.; Bouzayen, M.; Rombaldi, C.; Pech, J.C.; Latché, A..
Alcohol acyltransferases (AAT) play a key role in the biosynthesis of ester aroma volatiles in fruit. Three ripening-specific recombinant AATs of cantaloupe Charentais melon fruit (Cm-AAT1, Cm-AAT3, and Cm-AAT4) are capable of synthesizing thioether esters with Cm-AAT1 being by far the most active. All proteins, as well as AAT(s) extracted from melon fruit, are active as tetramers of around 200 kDa. Kinetic analysis demonstrated that CoA-SH, a product of the reaction, is an activator at low concentrations and an inhibitor at higher concentrations. This was confirmed by the addition of phosphotransacetylase at various concentrations, capable of modulating the level of CoA-SH in the reaction medium. Site-directed mutagenesis of some amino acids that were...
Tipo: Journal Article Palavras-chave: PROPRIETE BIOCHIMIQUE ALCOHOL ACYLTRANSFERASES; AROMA VOLATILES; SULFUR-CONTAINING ESTERS; SITE-DIRECTED MUTAGENESIS; ACTIVITE ENZYMATIQUE.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20073d83f2e9&uri=/notices/prodinra1/2007/10/
Imagem não selecionada

Imprime registro no formato completo
Change in anthocyanin concentrations in red apricot fruits during ripening Inra
Bureau, S.; Renard, C.M.G.C.; Reich, M.; Ginies, C.; Audergon, J.M..
Here we report on accumulation patterns of anthocyanins and of β-carotene during fruit maturation, between 82 and 125 days after flowering, of two apricot (Prunus armeniaca L.) cultivars, A3576 and A3751. Both cultivars displayed an intense red colour of the skin but differed in their genetic background. The pigments were extracted from skin and flesh, separately, and analysed using HPLC–DAD–MS. Out of three anthocyanins detected here, the major compound, cyanidin-3-O-rutinoside was present at 75%. The two minor compounds were cyanidin-3-O-glucoside and peonidin-3-O-rutinoside. This is the first time that peonidin-3-O-rutinoside has been detected in apricot fruit. During maturation, A3751 accumulated anthocyanins in both skin and flesh, whereas...
Tipo: Journal Article Palavras-chave: PROPRIETE BIOCHIMIQUE; PROPRIETE PHYSIQUE PIGMENTS; HPLC; CHEMICAL COMPOSITION; FIRMNESS; BETA-CAROTENE; HPLC-DAD-MS; ANTHOCYANIN.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008bd0807de&uri=/notices/prodinra1/2008/10/
Imagem não selecionada

Imprime registro no formato completo
CYP1A1 induction in the colon by serum: involvement of the PPARα pathway and evidence for a new specific human PPREα site Inra
Villard, P.H.; Barlesi, F.; Armand, M.; Dao, M.A.; Pascussi, J.M.; Fouchier, F.; Champion, S.; Dufour, C.; Ginies, C.; Ayman, K.; Amiot-Carlin, M.J.; Barra, Y.; Seree, É..
BackgroundWe previously showed that blood serum induced cytochrome P450 1A1 (CYP1A1) monooxygenase expression in vitro.ObjectiveOur purpose was (i) to identify the molecular mechanism involved and (ii) to characterize the inducer compound(s) in serum involved at least in part.MethodsSerum was fractionated on hydrophobic columns. PPARα involvement was demonstrated by gene reporter assays, DNA mutagenesis and EMSA. Gene expression was evaluated by qRT-PCR. Serum samples were analyzed using HS-SPME-GC-MS.ResultsThe inductive effect of serum did not depend on the AhR pathway and was enhanced by cotransfection of PPARα cDNA. Mutations in the PPAR response elements of the CYP1A1 gene promoter suppressed this effect. One of the PPRE sites appeared highly specific...
Tipo: Journal Article Palavras-chave: BLOOD SERUM; RT-PCR; INDUCTION; HEXANAL.
Ano: 2011 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201113910e75&uri=/notices/prodinra1/2011/05/
Imagem não selecionada

Imprime registro no formato completo
Eco-friendly and cleaner process for isolation of essential oil using microwave energy. Experimental and theoretical study Inra
Farhat, A.; Ginies, C.; Romdhane, M.; Chemat, F..
Microwave steam diffusion (MSD) was developed as a cleaner and new process design and operation for isolation of essentials oils and was compared to conventional steam diffusion (SD). The essential oils extracted by MSD for 3 min were quantitatively (yield) and qualitatively (aromatic profile) similar to those obtained by conventional steam diffusion for 20 min. In addition, an optimal operating steam flow rate of 25 g min―1 and microwave power 200W were found to ensure complete extraction yield with reduced extraction time. To confirm the efficiency of this process a mathematical model was proposed to describe the mass transfer of essential oil from lavender. Solid-steam mass transfer coefficients obtained by MSD were six times higher than obtained by SD....
Tipo: Journal Article Palavras-chave: MICROSCOPIE ELECTRONIQUE; ENTRAINEMENT A LA VAPEUR; EXTRACTION MICROONDE; HUILE ESSENTIELLE; TECHNOLOGIE PROPRE MICROWAVE DIFFUSION; ESSENTIAL OIL; STEAM DIFFUSION; GREEN ANALYSIS.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20097f58dc70&uri=/notices/prodinra1/2010/01/
Imagem não selecionada

Imprime registro no formato completo
Green procedure with a green solvent for fats and oils’ determination microwave-integrated Soxhlet using limonene followed by microwave clevenger distillation Inra
Virot, M.; Tomao, V.; Ginies, C.; Visinoni, F.; Chemat, F..
Here is described a green and original alternative procedure for fats and oils’ determination in oleaginous seeds. Extractions were carried out using a by-product of the citrus industry as extraction solvent, namely d-limonene, instead of hazardous petroleum solvents such as n-hexane. The described method is achieved in two steps using microwave energy: at first, extractions are attained using microwave-integrated Soxhlet, followed by the elimination of the solvent from the medium using a microwave Clevenger distillation in the second step. Oils extracted from olive seeds were compared with both conventional Soxhlet and microwave-integrated Soxhlet extraction procedures performed with n-hexane in terms of qualitative and quantitative determination. No...
Tipo: Journal Article Palavras-chave: DISTILLATION; MICROONDE; BIOSOLVANT MICROWAVE; LIMONENE; SOXHLET; CLEVENGER; EXTRACTION; BIO-SOLVENT.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008a6fd03ce&uri=/notices/prodinra1/2008/08/
Imagem não selecionada

Imprime registro no formato completo
Identification of Bacillus cereus genes specifically expressed during growth at low temperatures Inra
Brillard, J.; Jehanno, I.; Dargaignaratz, C.; Barbosa, I.; Ginies, C.; Carlin, F.; FEDHILA, S.; Nguyen The, C.; Broussolle, V.; Sanchis, V..
The mechanisms involved in the ability of B. cereus to multiply at low temperature were investigated. It was assumed that many genes involved in cold acclimation would be up-regulated at low temperature. Recombinase-based in vivo expression technology (IVET) was adapted to the detection of the transient activation of B. cereus promoters during growth at 10 °C. Four independent screenings of a promoter library from type strain ATCC 14579 were performed and 17 clones were isolated. They corresponded to 17 promoter regions that displayed a reproducible elevated expression at 10 °C relative to 30 °C. This analysis revealed several genes that may be important for B. cereus to grow successfully in the restrictive conditions of cold habitats. Among them, a locus...
Tipo: Journal Article Palavras-chave: IVET; FOOD BORNE BACTERIA; ENTEROTOXIN.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20106c1d95fa&uri=/notices/prodinra1/2010/04/
Imagem não selecionada

Imprime registro no formato completo
In vivo metabolism of diallyl disulphide in the rat : identification of two new metabolites Inra
Germain, E.; Auger, J.; Ginies, C.; Siess, M.H.; Teyssier, C..
1. Diallyl disulphide (DADS), a compound formed from the organosulphur compounds present in garlic, is known for its anticarcinogenic effects in animal models.2. The aim was to identify and analyse the metabolites produced in vivo after a single oral administration of 200 mg kg -1 DADS to rats. The organic sulphur metabolites present in the stomach, liver, plasma and urine were measured by gas chromatography coupled with mass spectrometry over 15 days.3. Data indicate that DADS is absorbed and transformed into allyl mercaptan, allyl methyl sulphide, allyl methyl sulphoxide (AMSO) and allyl methyl sulphone (AMSO 2 ), which are detected throughout the excretion period. Overall, the highest amounts of metabolites were measured 48-72h after the DADS...
Tipo: Journal Article Palavras-chave: AIL; DADS.
Ano: 2002 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200720a8e6d&uri=/notices/prodinra1/2008/10/
Imagem não selecionada

Imprime registro no formato completo
Mechanisms of melon fruit ripening and development of sensory quality National Institute of Agronomic Research
Ayub, R.; Rombaldi, C.; Lucchetta, L.; Ginies, C.; Latché A., A.; Bouzayen, M.; Pech, J.C..
This paper is aimed at reviewing some of our data obtained in the recent years on the mechanisms of melon fruit ripening and development of fruit sensory quality, with emphasis on fruit softening and biosynthesis of aroma volatiles. Ethylene is playing a major role in the ripening of climacteric fruit such as Cantaloupe Charentais melon. However, knocking-down the expression of ACC oxidase, a gene involved in the last step of ethylene biosynthesis, has shown that several ripening pathways and sensory attributes were ethylene-independent. Fruit softening, an important quality parameter has a strong ethylene-dependent component and a minor ethyleneindependent component. It is associated with cell wall disassembly which involves the breakdown of many...
Tipo: Conference Paper Palavras-chave: Cucumis melo; Melon; Ethylene; Aroma volatiles; Softening; Cell-wall degrading gene; Fruit sensory quality.
Ano: 2008 URL: http://hdl.handle.net/2174/214
Imagem não selecionada

Imprime registro no formato completo
Microwave-integrated extraction of total fats and oils Inra
Virot, M.; Tomao, V.; Ginies, C.; Visinoni, F.; Chemat, F..
An improved process of Soxhlet extraction assisted by microwave, called microwave-integrated Soxhlet (MIS) is proposed for the extraction of oils and fats from different food matrixes such as oleaginous seeds, meat and bakery products. Optimal conditions for extraction were obtained using a response surface methodology and reached from a central composite design allowing us to conclude in a previous paper that the proposed process ensures complete, efficient and accurate extraction for lipids determination from olives. In this paper, the peak areas of the main fatty acids extracted with MIS from olive seeds were considered as response variables and submitted to an analysis of variance in order to determine if there was a significant link between the...
Tipo: Journal Article Palavras-chave: MICROONDE; PROCEDE SOXHLET; MICROWAVE INTEGRATED SOXHLET; PENTA-HEXANE; FOOD; GAS CHROMATOGRAPHY; MASS SPECTROMETRY.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20082c736495&uri=/notices/prodinra1/2008/08/
Imagem não selecionada

Imprime registro no formato completo
(Z,Z,E)-3,6,8-dodecatrien-1-ol, a major component of trail-following pheromone in two sympatric termite species Reticulitermes lucifugus grassei and R. santonensis Inra
Wobst, B.; Farine, J.P.; Ginies, C.; Sémon, E.; Robert, A.; Bonnard, O.; Connétable, S.; Bordereau, C..
Trail-following bioassays show that trails of the two subterranean termites, Reticulitermes lucifugus grassei and R. santonensis, which are sympatric in some areas of southwestern France, are not species specific. Even when tested just above threshold level, trails from pentane extracts of whole workers of R. santonensis are always preferred by both species. If the R. santonensis extract is progressively diluted, the preference is lost. In purified extracts of workers of R. lucifugus grassei that elicited a trail-following response, only one compound is active. It was identified by GC-MS as the same major compound of the trail-following pheromone of R. santonensis: (Z,Z,E)-3,6,8-dodecatrien-1-ol (DTE-OH). The threshold concentration of activity of...
Tipo: Journal Article Palavras-chave: TERMITE; RETICULITERME LUCIFUGUS GRASSEI; RETICULITERME SANTONENSIS; PHEROMONE SEXUELLE; COMPOSITION CHIMIQUE; COMMUNICATION ANIMALE TRAIL-FOLLOWING PHEROMONES; (Z Z E)-3 6 8-DODECATRIEN-1-OL; SPECIFICITY; TERMITES; RETICULITERMES LUCIFUGUS GRASSEI; RETICULITERMES SANTONENSIS; GC-MS.
Ano: 1999 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0000033887079664&uri=/notices/prodinra1/2010/11/
Registros recuperados: 10
Primeira ... 1 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional