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A simplified DNA extraction method for PCR analysis of Camarotella spp. BABT
Vitória,Nadja Santos; Bezerra,José Luiz; Gramacho,Karina Peres.
This work aimed to optimize an efficient and simple protocol for DNA extraction of Camarotella species, an obligate plant pathogen that cause verrucosis or "lixa" on coconut tree and other palms, facilitating the molecular studies of these biotrophic microorganisms. The method proposed enabled a fast, reproducible and reliable DNA extraction from Camarotella species.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Camarotella torrendiella; C. acrocomiae; Verrucosis on coconut; DNA; PCR.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200001
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Analysis of the ergosterol biosynthesis pathway cloning, molecular characterization and phylogeny of lanosterol 14α-demethylase (ERG11) gene of Moniliophthora perniciosa Genet. Mol. Biol.
Ceita,Geruza de Oliveira; Vilas-Boas,Laurival Antônio; Castilho,Marcelo Santos; Carazzolle,Marcelo Falsarella; Pirovani,Carlos Priminho; Selbach-Schnadelbach,Alessandra; Gramacho,Karina Peres; Ramos,Pablo Ivan Pereira; Barbosa,Luciana Veiga; Pereira,Gonçalo Amarante Guimarães; Góes-Neto,Aristóteles.
The phytopathogenic fungus Moniliophthora perniciosa (Stahel) Aime & Philips-Mora, causal agent of witches' broom disease of cocoa, causes countless damage to cocoa production in Brazil. Molecular studies have attempted to identify genes that play important roles in fungal survival and virulence. In this study, sequences deposited in the M. perniciosa Genome Sequencing Project database were analyzed to identify potential biological targets. For the first time, the ergosterol biosynthetic pathway in M. perniciosa was studied and the lanosterol 14α-demethylase gene (ERG11) that encodes the main enzyme of this pathway and is a target for fungicides was cloned, characterized molecularly and its phylogeny analyzed. ERG11 genomic DNA and cDNA were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Basidiomycota; Fungus; Ergosterol; Theobroma cacao.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500011
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