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Application of microsatellite markers for breeding and genetic conservation of herds of Pantaneiro sheep Electron. J. Biotechnol.
Crispim,Bruno do Amaral; Seno,Leonardo de Oliveira; Egito,Andréa Alves do; Vargas Junior,Fernando Miranda de; Grisolia,Alexéia Barufatti.
Background The aim of the present study was to assess the genetic diversity of Pantaneiro sheep, using microsatellite markers, in order to assist maintenance and management plans, enhance mating systems and reduce the inbreeding rate. A total of 127 animals were genotyped at eight microsatellite loci. They belonged to populations from the Experimental Farm of the Universidade Federal da Grande Dourados (UFGD) (Dourados/MS/Brazil) and Embrapa Pantanal (Corumbá/MS/Brazil). Results The population of Pantaneiro sheep from the UFGD exhibited a high mean number of alleles (11.13) and allelic richness (10.66). The polymorphic information content was highly informative in the locus studied, resulting in a mean value of 0.71. Observed heterozygosity was lower than...
Tipo: Journal article Palavras-chave: Animal genetic resources; Genetic diversity; Kinship; Locally adapted breed; STR.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600010
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Genetic identification of interspecific hybrid of Neotropical catfish species (Pseudoplatystoma corruscans vs. Pseudoplatystoma reticulatum) in rivers of Mato Grosso do Sul State, Brazil Neotropical Ichthyology
Vaini,Jussara Oliveira; Grisolia,Alexéia Barufatti; Prado,Fernanda Dotti do; Porto-Foresti,Fábio.
Hybrids of the species Pseudoplatystoma corruscansvs.Pseudoplatystoma reticulatum are extensively traded because they are more docile and precocious compared to their parents. This study aimed to identify the occurrence of hybrid 'Surubim' in rivers of the State of Mato Grosso do Sul, applying molecular techniques. Pseudoplatystoma corruscans, Pseudoplatystoma reticulatum and hybrid 'Surubim' were detected using multiplex PCR and PCR-RFLP in RAG2, GLOBIN, EF1α, 18S rRNA nuclear genes, and mitochondrial 16S rRNA gene. The results revealed the presence of F1 hybrids in rivers and the occurrence of genetic introgression, evidenced by the Post-F1 hybrids. Furthermore, discrepancies between the morphological and genetic identification were observed,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Fish; Hybridization; Introgression; Molecular markers.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000300635
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Genetic variability of Herpailurus yagouaroundi, Puma concolor and Panthera onca (Mammalia, Felidae) studied using Felis catus microsatellites Genet. Mol. Biol.
Moreno,Vanessa Roma; Grisolia,Alexéia Barufatti; Campagnari,Francine; Milazzotto,Marcela; Adania,Cristina Harumi; Garcia,José Fernando; Souza,Edislane Barreiros de.
We used four microsatellite loci (Fca08, Fca45, Fca77 and Fca96) from the domestic cat, Felis catus, to investigate genetic variability in specimens of Herpailurus yagouaroundi (jaguarundi, otter cat, eyra), Puma concolor (cougar, mountain lion, puma) and Panthera onca (jaguar) held in various Brazilian zoos. Samples of DNA from the cats were PCR amplified and then sequenced before being analyzed using the CERVUS program. Our results show a mean polymorphic information content (PIC) of 0.83 for H. yagouaroundi, 0.66 for P. concolor and 0.69 for P. onca and a mean of 10.3 alleles for the Fca08 locus, 5.3 for Fca 45, 9 for Fca 77 and 14 for Fca 96. These results indicate a relatively high level of genetic diversity for the specimens studied.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic variability; Mammals; Felines; Microsatellites.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200017
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