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Determination of lytic enzyme activities of indigenous Trichoderma isolates from Pakistan BJM
Asad,Saeed Ahmad; Tabassum,Ayesha; Hameed,Abdul; Hassan,Fayyaz ul; Afzal,Aftab; Khan,Sabaz Ali; Ahmed,Rafiq; Shahzad,Muhammad.
Abstract This study investigated lytic enzyme activities in three indigenous Trichoderma strains namely, Trichoderma asperellum, Trichoderma harzianum and Trichoderma sp. Native Trichoderma strains and a virulent strain of Rhizoctonia solani isolated from infected bean plants were also included in the study. Enzyme activities were determined by measuring sugar reduction by dinitrosalicylic acid (DNS) method using suitable substrates. The antagonists were cultured in minimal salt medium with the following modifications: medium A (1 g of glucose), medium B (0.5 g of glucose + 0.5 g of deactivated R. solani mycelia), medium C (1.0 g of deactivated respective antagonist mycelium) and medium D (1 g of deactivated R. solani mycelia). T asperellum showed presence...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lytic enzymes; Mycoparasitic activity; Rhizoctonia solani; Trichoderma.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401053
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Ethambutol resistance of indigenous Mycobacterium tuberculosis isolated from human patients BJM
Nazir,Taha; Rasool,Muhammad Hidayat; Hameed,Abdul; Ahmad,Bashir; Qureshi,Javed Anver.
The present study was conducted to find out the ethambutol resistance pattern of indigenous isolates of Mycobacterium tuberculosis from Tuberculosis diagnosed human patients. A total of 172 specimens were collected from six different sources and comprised of 84.9% sputum, 10.5% pus and 4.7% bronchial washings. There were 70.9% males and 29.1% females with 84.30% pulmonary and 15.69% extra-pulmonary tuberculosis. The Mycobacterium tuberculosis isolates collected from primary culture were further studied to determine their pattern and level of resistance. The inoculums were prepared using 0.5 Mac Farland turbidity standards. Five different concentration of ethambutol were used in Lowenstein Jensen (LJ) medium i.e. 2μg/ml, 4μg/ml, 6μg/ml, 8μg/ml and 10μg/ml...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ethambutol; Mycobacterium tuberculosis; Resistance; Lowenstein Jensen medium; Human patients.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400026
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Production of peptide antibiotics by Bacillus sp: GU 057 indigenously isolated from saline soil BJM
Amin,Adnan; Khan,Muhammad Ayaz; Ehsanullah,Malik; Haroon,Uzma; Azam,Sheikh Muhammad Farooq; Hameed,Abdul.
A total of 112 soil samples were taken from differents areas of district D.I.Khan and Kohat (KPK) Pakistan and screened for production of antibiotics against the Micrococcus luteus and Staphylococcus aureus. Widest zone of inhibition (18mm) was produced by microorganism isolated from saline soil. The strain was later identified as Bacillus GU057 by standard biochemical assays. Maximum activity (18mm inhibition zone) was observed against Staphylococcus aureus after 48 hours of incubation at pH 8 and 4% concentration of glucose. The antibiotic was identified by autobiography as bacitracin. The Bacillus strain GU057 was confirmed as good peptide antibiotic producer and can effectively be indulged as biocontrol agent.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peptide antibiotics; Bacillus species; Screening; Optimization of parameters.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400015
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Role of brown-rot fungi in the bioremoval of azo dyes under different conditions BJM
Ali,Naeem; Hameed,Abdul; Ahmed,Safia.
The present study is vital to the understanding of bioremediation of structurally different azo dyes by some unusual Brown-rot fungi. Bioremoval of each dye (20 mg l-1) was tested in two different culture media under static and shaking conditions by taking inocula from different fungi. Fungal strains showed varying dyes removal abilities, though considerable high in case of Acid Red (AR) 151(di-azo) as compared to Orange (Or) II (mono-azo). With an exception of Aspergillus tereus SA3, all the fungal isolates showed higher removal of dyes in SDB. Under static condition, the maximum decolorizing fungal strains were; Aspergillus flavus SA2 (67%) and Alternaria spp. SA4 (57%) in AR 151, while Penicillium spp. (34 and 33 %) in Orange II, in SDB and STE,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Azo dyes; Bio-removal; Bio-decolorization; Culture conditions; Fungi.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400009
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