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Registros recuperados: 11
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3D arrays on quantum dots embedded within a bio-inspired template studied by SAXS ArchiMer
Henry, Etienne; Dif, A.; Schmutz, M.; Legoff, L.; Amblard, F.; Marchi, V.; Artzner, F..
Tipo: Text
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00206/31758/30166.pdf
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A cooperative and specific DNA-binding mode of HIV-1 integrase depends on the nature of the metallic cofactor and involves the zinc-containing N-terminal domain ArchiMer
Carayon, Kevin; Leh, Herve; Henry, Etienne; Simon, Francoise; Mouscadet, Jean-francois; Deprez, Eric.
HIV-1 integrase catalyzes the insertion of the viral genome into chromosomal DNA. We characterized the structural determinants of the 3'-processing reaction specificity-the first reaction of the integration process-at the DNA-binding level. We found that the integrase N-terminal domain, containing a pseudo zinc-finger motif, plays a key role, at least indirectly, in the formation of specific integrase-DNA contacts. This motif mediates a cooperative DNA binding of integrase that occurs only with the cognate/viral DNA sequence and the physiologically relevant Mg(2+) cofactor. The DNA-binding was essentially non-cooperative with Mn(2+) or using non-specific/random sequences, regardless of the metallic cofactor. 2,2'-Dithiobisbenzamide-1 induced zinc ejection...
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Ano: 2010 URL: http://archimer.ifremer.fr/doc/00206/31773/30183.pdf
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Calcium-driven DNA synthesis by a high-fidelity DNA polymerase ArchiMer
Ralec, Celine; Henry, Etienne; Lemor, Melanie; Killelea, Tom; Henneke, Ghislaine.
Divalent metal ions, usually Mg2+, are required for both DNA synthesis and proofreading functions by DNA polymerases (DNA Pol). Although used as a non-reactive cofactor substitute for binding and crystallographic studies, Ca2+ supports DNA polymerization by only one DNA Pol, Dpo4. Here, we explore whether Ca2+-driven catalysis might apply to high-fidelity (HiFi) family B DNA Pols. The consequences of replacing Mg2+ by Ca2+ on base pairing at the polymerase active site as well as the editing of terminal nucleotides at the exonuclease active site of the archaeal Pyrococcus abyssi DNA Pol (PabPolB) are characterized and compared to other (families B, A, Y, X, D) DNA Pols. Based on primer extension assays, steady-state kinetics and ion-chased experiments, we...
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Ano: 2017 URL: http://archimer.ifremer.fr/doc/00405/51663/52210.pdf
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Comparative Study of the Fatty Acid Binding Process of a New FABP from Cherax quadricarinatus by Fluorescence Intensity, Lifetime and Anisotropy ArchiMer
Li, Jiayao; Henry, Etienne; Wang, Lanmei; Delelis, Olivier; Wang, Huan; Simon, Francoise; Tauc, Patrick; Brochon, Jean-claude; Zhao, Yunlong; Deprez, Eric.
Fatty acid-binding proteins (FABPs) are small cytosolic proteins, largely distributed in invertebrates and vertebrates, which accomplish uptake and intracellular transport of hydrophobic ligands such as fatty acids. Although long chain fatty acids play multiple crucial roles in cellular functions (structural, energy metabolism, regulation of gene expression), the precise functions of FABPs, especially those of invertebrate species, remain elusive. Here, we have identified and characterized a novel FABP family member, Cq-FABP, from the hepatopancreas of red claw crayfish Cherax quadricarinatus. We report the characterization of fatty acid-binding affinity of Cq-FABP by four different competitive fluorescence-based assays. In the two first approaches, the...
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00206/31770/30186.pdf
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Contrastes de la motorizacion y de la movilidad en las megapolis IRD
Henry, Etienne; Hubert, J.P. (collab.).
La motorisation des déplacements quotidiens qui accompagne l'urbanisme de la société paraît être une tendance historique inéluctable, avec ses avantages et dysfonctionnements respectifs. L'expérience latino-américaine, aux taux élevés d'urbanisation, peut servir de module comparatif avec des pays du Nord et du Sud. Dans ces métropoles, on assiste à certaine substitution des moyens collectifs de transport par les modes individuels, amenant d'inédites situations de congestion, de pollution et d'insécurité. La mobilité ne paraît pas augmenter à un rythme conforme au processus d'urbanisation, selon les résultats produits par quelques enquêtes ménage (ici, huit métropoles). Certaines expériences répétées d'enquêtes origine destination fournissent des...
Tipo: Text Palavras-chave: TRANSPORT URBAIN; URBANISATION; MEGAPOLE; TRANSPORT COLLECTIF; DEPLACEMENT; VIE QUOTIDIENNE; POLLUTION; MENAGE; REVENU; SEGREGATION; CONTRASTE; ENQUETE; TRANSPORT INDIVIDUEL; MOBILITE URBAINE.
Ano: 2000 URL: http://www.documentation.ird.fr/hor/fdi:010023428
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Differential Activities of DNA Polymerases in Processing Ribonucleotides during DNA Synthesis in Archaea ArchiMer
Lemor, Melanie; Kong, Ziqing; Henry, Etienne; Brizard, Raphael; Laurent, Sebastien; Bosse, Audrey; Henneke, Ghislaine.
Consistent with the fact that ribonucleotides (rNTPs) are in excess over deoxyribonucleotides (dNTPs) in vivo, recent findings indicate that replicative DNA polymerases (DNA Pols) are able to insert ribonucleotides (rNMPs) during DNA synthesis, raising crucial questions about the fidelity of DNA replication in both Bacteria and Eukarya. Here, we report that the level of rNTPs is 20-fold higher than that of dNTPs in Pyrococcus abyssi cells. Using dNTP and rNTP concentrations present in vivo, we recorded rNMP incorporation in a template-specific manner during in vitro synthesis, with the family-D DNA Pol (PolD) having the highest propensity compared with the family-B DNA Pol and the p41/p46 complex. We also showed that ribonucleotides accumulate at a...
Tipo: Text Palavras-chave: Archaea; DNA replication and repair; DNA polymerase; Nucleotide pool; Translesion synthesis.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00464/57603/59796.pdf
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Envahir, conseiller et gouverner...la ville d'Amérique latine IRD
Henry, Etienne; Sachs-Jeantet, C..
Tipo: Text Palavras-chave: VILLE; URBANISATION; BIDONVILLE; MARGINALITE; MOUVEMENTS SOCIAUX URBAINS; POUVOIR LOCAL; GESTION URBAINE; SECTEUR INFORMEL; SOCIOLOGIE URBAINE; RECHERCHE URBAINE; SERVICES DE BASE URBAINS; CITOYENNETE.
Ano: 1993 URL: http://www.documentation.ird.fr/hor/fdi:37759
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Mechanistic insight into cadmium-induced inactivation of the Bloom protein ArchiMer
Qin, Wei; Bazeille, Nicolas; Henry, Etienne; Zhang, Bo; Deprez, Eric; Xi, Xu-guang.
Cadmium is a toxic metal that inactivates DNA-repair proteins via multiple mechanisms, including zinc substitution. In this study, we investigated the effect of Cd2+ on the Bloom protein (BLM), a DNA-repair helicase carrying a zinc-binding domain (ZBD) and playing a critical role to ensure genomic stability. One characteristics of BLM-deficient cells is the elevated rate of sister chromatid exchanges, a phenomenon that is also induced by Cd2+. Here, we show that Cd2+ strongly inhibits both ATPase and helicase activities of BLM. Cd2+ primarily prevents BLM-DNA interaction via its binding to sulfhydryl groups of solvent-exposed cysteine residues and, concomitantly, promotes the formation of large BLM multimers/aggregates. In contrast to previously described...
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Ano: 2016 URL: https://archimer.ifremer.fr/doc/00341/45196/44599.pdf
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Mitochondria-targeted Triphenylamine Derivatives Activatable by Two-Photon Excitation for Triggering and Imaging Cell Apoptosis ArchiMer
Chennoufi, Rahima; Bougherara, Houcine; Gagey-eilstein, Nathalie; Dumat, Blaise; Henry, Etienne; Subra, Frederic; Bury-mone, Stephanie; Mahuteau-betzer, Florence; Tauc, Patrick; Teulade-fichou, Marie-paule; Deprez, Eric.
Photodynamic therapy (PDT) leads to cell death by using a combination of a photosensitizer and an external light source for the production of lethal doses of reactive oxygen species (ROS). Since a major limitation of PDT is the poor penetration of UV-visible light in tissues, there is a strong need for organic compounds whose activation is compatible with near-infrared excitation. Triphenylamines (TPAs) are fluorescent compounds, recently shown to efficiently trigger cell death upon visible light irradiation (458 nm), however outside the so-called optical/therapeutic window. Here, we report that TPAs target cytosolic organelles of living cells, mainly mitochondria, triggering a fast apoptosis upon two-photon excitation, thanks to their large two-photon...
Tipo: Text Palavras-chave: Apoptosis; Small molecules.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00320/43168/42726.pdf
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Physical and functional interplay between PCNA DNA clamp and Mre11–Rad50 complex from the archaeon Pyrococcus furiosus ArchiMer
Hogrel, Gaelle; Lu, Yang; Laurent, Sebastien; Henry, Etienne; Etienne, Clarisse; Duy Khanh Phung,; Dulermo, Remi; Bosse, Audrey; Pluchon, Pierre Francois; Clouet-d'Orval, Beatrice; Flament, Didier.
Several archaeal species prevalent in extreme environments are particularly exposed to factors likely to cause DNA damages. These include hyperthermophilic archaea (HA), living at temperatures >70°C, which arguably have efficient strategies and robust genome guardians to repair DNA damage threatening their genome integrity. In contrast to Eukarya and other archaea, homologous recombination appears to be a vital pathway in HA, and the Mre11–Rad50 complex exerts a broad influence on the initiation of this DNA damage response process. In a previous study, we identified a physical association between the Proliferating Cell Nuclear Antigen (PCNA) and the Mre11–Rad50 (MR) complex. Here, by performing co-immunoprecipitation and SPR analyses, we identified a...
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Ano: 2018 URL: http://archimer.ifremer.fr/doc/00439/55092/56534.pdf
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Structure of the DP1–DP2 PolD complex bound with DNA and its implications for the evolutionary history of DNA and RNA polymerases ArchiMer
Raia, Pierre; Carroni, Marta; Henry, Etienne; Pehau-arnaudet, Gerard; Brule, Sebastien; Beguin, Pierre; Henneke, Ghislaine; Lindahl, Erik; Delarue, Marc; Sauguet, Ludovic.
PolD is an archaeal replicative DNA polymerase (DNAP) made of a proofreading exonuclease subunit (DP1) and a larger polymerase catalytic subunit (DP2). Recently, we reported the individual crystal structures of the DP1 and DP2 catalytic cores, thereby revealing that PolD is an atypical DNAP that has all functional properties of a replicative DNAP but with the catalytic core of an RNA polymerase (RNAP). We now report the DNA-bound cryo–electron microscopy (cryo-EM) structure of the heterodimeric DP1–DP2 PolD complex from Pyrococcus abyssi, revealing a unique DNA-binding site. Comparison of PolD and RNAPs extends their structural similarities and brings to light the minimal catalytic core shared by all cellular transcriptases. Finally, elucidating the...
Tipo: Text
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00477/58883/61420.pdf
Registros recuperados: 11
Primeira ... 1 ... Última
 

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