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| Montealegre,Jaime; Valderrama,Luis; Herrera,Rodrigo; Besoaín,Ximena; Pérez,Luz M. |
| Wild (Th11, Th12 and Th650) and mutant (Th11A80.1, Th12A40.1, Th12C40.1 and Th650-NG7) Trichoderma harzianum strains were stored for 180 days at 5ºC or at 22ºC, in two types of soils. Strains recovered at 90, 120 and 180 days from the two types of soils, retained their full capacity to biocontrol Rhizoctonia solani 618, that produces crown and root rot of tomatoes. Recovery, estimated as colony forming units (cfu) of the wild and mutant strains, showed that all increased their cfu after storage independently of the type of soil and temperature, although kinetic behavior differed among strains. Ratios of recovery after storage in type B soil/ type A soil or at 22ºC/5ºC, higher or lower than one respectively, allowed to establish that Th11 and Th12 were the... |
| Tipo: Journal article |
Palavras-chave: Bioantagonism; Persistence; Root rot; Tomato. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400002 |
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| Montealegre,Jaime R; Herrera,Rodrigo; Velásquez,Juan Carlos; Silva,Polyana; Besoaín,Ximena; Pérez,Luz María. |
| Trichoderma harzianum 650 (Th650) and Paenebacillus lentimorbus 629 (Pl629) selected earlier for their ability to control Rhizoctonia solani, Fusarium solani and F. oxysporum in vitro, were applied alone or combined with solarization (summer assay) and/or with methyl bromide (MeBr) (summer and winter assays) to a soil with a high inoculum level, for the control of tomato root rot caused by the complex F. oxysporum f. sp. lycopersici - Pyrenochaeta lycopersici - Rhizoctonia solani. Evaluations were also performed independently for root damage caused by P. lycopersici, and also for R. solani in the summer assay. MeBr decreased tomato root damage caused by the complex from 88.7% to 21.2% and from 78.4% to 35.7% in the summer and in the winter assay,... |
| Tipo: Journal article |
Palavras-chave: Fusarium oxysporum; Lycopersicon esculentum; Pyrenochaeta lycopersici; Rhizoctonia solani; Solarization. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300004 |
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| Montealegre,Jaime; Valderrama,Luis; Sánchez,Soledad; Herrera,Rodrigo; Besoain,Ximena; Pérez,Luz María. |
| Biocontrol of Rhizoctonia solani in tomatoes cultivated under greenhouse and field conditions was analyzed using the Trichoderma harzianum mutants Th650-NG7, Th11A80.1, Th12A40.1, Th12C40.1 and Th12A10.1 and ThF2-1, respectively. Their innocuousness on tomato cultivars 92.95 and Gondola (greenhouse assays), and on cultivar Fortaleza (field assays) was established. Alginate pellets (1.7 g pellets/L soil) containing c.a1 x 10(5) colony forming units (cfu)/g pellet were applied to a soil previously inoculated with R. solani at transplant (greenhouse) or to a naturally infected soil (field). Controls considered parental wild strains, a chemical fungicide and no additions. Th11A 80.1, Th12A10.1 and Th650-NG7 prevented the 100% mortality of tomato plants cv.... |
| Tipo: Journal article |
Palavras-chave: Bioantagonism; Field assays; Greenhouse assays; Root rot; Tomato. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200001 |
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| Montealegre,Jaime R; Ochoa,Fabián; Besoain,Ximena; Herrera,Rodrigo; Pérez,Luz M. |
| J.R. Montealegre, F. Ochoa, X. Besoain, R. Herrera, and L.M. Pérez. 2014. In vitro and glasshouse biocontrol of Rhizoctonia solani with improved strains of Trichoderma spp. Cien. Inv. Agr. 41(2):197-206. The potential of Trichoderma spp. fusants for the biocontrol of Rhizoctonia solani was compared with the ability of their corresponding parental strains. Their effect was tested in vitro using two R. solani strains, 509 (AG 2-1) and 618 (AG 4). The highest inhibitions in growth in dual cultures were obtained with the ThF2-1 (89.79%), ThF3-3 (90.55%), ThF4-15 (91.75%) and ThF5-8 (77.67%) fusants on R. solani 509; only ThF2-1 was able to inhibit the growth of R. solani 618 (60.19%). The inhibitory effect on growth was mainly due to diffusible metabolites.... |
| Tipo: Journal article |
Palavras-chave: Biological control; Methyl bromide; Tomato; Solanum lycopersicum. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202014000200006 |
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| Montealegre,Jaime R.; Reyes,Rodrigo; Pérez,Luz María; Herrera,Rodrigo; Silva,Polyana; Besoain,Ximena. |
| Bacteria from the rhizoplane and surrounding soil of healthy and Rhizoctonia solani diseased tomato plants, cropped in greenhouse of the V Region of Chile, were collected. The best bacterial strains, based on their ability to control development of three R. solani isolates (identified as belonging to the anastomosis groups AG-2-1, AG‑4), were identified as B. subtilis (one isolate) and B. lentimorbus (two different isolates). All bacterial isolates resulted effective for the in vitro control of growth of all R. solani isolates, where the control mechanisms used by the bacteria do not involve the secretion of fungal cell wall hydrolytic enzymes. R. solani AG-2-1 was more sensitive than R. solani AG-4. On the other hand, all bacteria grew well in... |
| Tipo: Journal article |
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| Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000200006 |
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| Herrera,Rodrigo; Núñez,David; Romero,Natalia; Besoain,Ximena; Pérez,Luz María; Montealegre,Jaime. |
| The germination of conidia in wild-type (Th11, Th12 and Th650) and mutant (Th11A80.1, Th12A10.1 and Th650-NG7) strains of Trichoderma harzianum that were exposed to different commercial fungicides was studied. All wild-type and mutant Trichoderma strains were germinated in the presence of 1,700 mg L-1 of pencycuron. The wild-type strains Th12 and Th650 and the corresponding mutant strains Th12A10.1 and Th650-NG7 were sensitive to all concentrations of iprodione and metalaxil + mancozeb. The EC50 (Median Effective Concentration) values for the different fungicides were between 10-1 and 10-4 less than the concentrations recommended for field applications; one exception was Phyto-fos on Th650-NG7, where this ratio was 0.72. These results establish whether... |
| Tipo: Journal article |
Palavras-chave: Biocontrol; Biocontrol agents; Control of tomato root diseases; Fungicides; Fungicide tolerant Trichoderma; Integrated management. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300016 |
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