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Registros recuperados: 7
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Development and characterization of 20 polymorphic microsatellite markers for Epinephelus marginatus (Lowe, 1834) (Perciformes: Epinephelidae) using 454 pyrosequencing Genet. Mol. Biol.
Vaini,Jussara Oliveira; Mota,Kenneth Gabriel; Ojeda,Alejandra Paola; Barreiros,João Pedro; Moreira,Renata Guimarães; Hilsdorf,Alexandre Wagner Silva.
Abstract The dusky grouper, Epinephelus marginatus, is a well-known and widespread marine fish assessed as endangered by the International Union for the Conservation of Nature. Analyzing the genetic diversity of this species is, therefore, of utmost importance and necessary for conservation purposes. Microsatellites are molecular tools with advantages that are ideal for population analyses. This study provides the first set of species-specific microsatellite loci for E. marginatus that can be applied when assessing both intra- and interpopulation genetic variation. Twenty microsatellite loci were isolated and characterized for the dusky grouper by genotyping 20 individuals obtained from the North Eastern Atlantic Ocean (n = 4) and from the South Western...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Short tandem repeat; Population genetics; Conservation; Fishery; Marine resources.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100074
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Genetic structuring of Salminus hilarii Valenciennes, 1850 (Teleostei: Characiformes) in the rio Paraná basin as revealed by microsatellite and mitochondrial DNA markers Neotropical Ichthyology
Silva,Juliana Viana da; Hallerman,Eric M.; Orfão,Laura Helena; Hilsdorf,Alexandre Wagner Silva.
Genetic variation of Salminus hilarii was assessed by screening microsatellite loci and mitochondrial D-loop DNA across four sampling in the upper rio Paraná basin of Brazil. Genetic diversity - measured as mean expected heterozygosity (0.904) and mean number of alleles across populations (13.7) - was reasonably high. Differentiation of microsatellite allele frequencies among populations was shown to be low but significant by AMOVA Φ ST (0.0192), and high by D EST (0.185). D-loop variation was high, with haplotypic diversity of 0.950 and nucleotide diversity of 0.011. Mitochondrial DNA-based estimates for population differentiation were high, with an overall Φ ST of 0.173. The results of tests of nuclear and mitochondrial variation yielded no unequivocal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: D-loop; River disruption; STR; Tabarana.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000300547
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Microsatellite variation and population genetic structure of a neotropical endangered Bryconinae species Brycon insignis Steindachner, 1877: implications for its conservation and sustainable management Neotropical Ichthyology
Matsumoto,Cristianne Kayoko; Hilsdorf,Alexandre Wagner Silva.
Piabanha (Brycon insignis) is a freshwater fish species from the drainages in Southeastern Brazil. During the 1950s, it was an important economic and food resource for local populations, but dramatic and continuous environmental degradation seriously jeopardized the B. insignis populations in the region. Microsatellite markers were used to assess the genetic structure of wild populations of B. insignis and compare the genetic variability and integrity of the wild populations with a captive population. Samples of DNA from 208 specimens from geographically isolated populations were analyzed. Population genetic structure was investigated using F ST, R ST estimates as well as AMOVA. All five loci used in this study were polymorphic with observed heterozygosity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Piabanha; Paraíba do Sul basin; Conservation; STR; Fish.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000300006
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Molecular assessment of Gymnotus spp. (Gymnotiformes: Gymnotidae) fishing used as live baitfish in the Tietê River, Brazil Neotropical Ichthyology
Faria-Pereira,Lilian Paula; Hilsdorf,Alexandre Wagner Silva; Albert,James; Paiva,Maria José Tavares Ranzani; Galvão,Márcia Santos Nunes.
ABSTRACT The capture of live bait for sport fishing is an important activity for fishing communities. The main species used for this purpose are members of the genus Gymnotus, which comprises numerous species of cryptic nature that are difficult to identify based on external morphology. The aims of this work were to identify through partial sequences of the COI gene Gymnotus species fished in the Jacaré-Guaçu River, SP, and to develop a molecular diagnostic approach using PCR-RFLP to identify these species. Partial COI sequences were compared to those of other species deposited in GenBank. The sequences were assessed in the NEBCutter program to determine restriction sites in the sequence and the enzymes to be tested. Phenetic analysis performed by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular identification; PCR-RFLP; Tuvira.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000500205
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Molecular cloning and sequence analysis of growth hormone cDNA of Neotropical freshwater fish Pacu (Piaractus mesopotamicus) Genet. Mol. Biol.
Pinheiro,Janeth Silva; Wolff,José Luiz Caldas; Araújo,Ronaldo de Carvalho; Hilsdorf,Alexandre Wagner Silva.
RT-PCR was used for amplifying Piaractus mesopotamicus growth hormone (GH) cDNA obtained from mRNA extracted from pituitary cells. The amplified fragment was cloned and the complete cDNA sequence was determined. The cloned cDNA encompassed a sequence of 543 nucleotides that encoded a polypeptide of 178 amino acids corresponding to mature P. mesopotamicus GH. Comparison with other GH sequences showed a gap of 10 amino acids localized in the N terminus of the putative polypeptide of P. mesopotamicus. This same gap was also observed in other members of the family. Neighbor-joining tree analysis with GH sequences from fishes belonging to different taxonomic groups placed the P. mesopotamicus GH within the Otophysi group. To our knowledge, this is the first GH...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Piaractus mesopotamicus; Growth hormone; CDNA cloning; Sequencing.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200037
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Molecular evidence of two cryptic species of Stramonita (Mollusca, Muricidae) in the southeastern Atlantic coast of Brazil Genet. Mol. Biol.
De Biasi,Juliana Beltramin; Tomás,Acácio Ribeiro Gomes; Hilsdorf,Alexandre Wagner Silva.
Abstract Snails of the genus Stramonita are commonly found in the rocky intertidal habitat of the western Atlantic Ocean coast. They belong to a monophyletic taxon that occurs along the tropical and warm-temperate Atlantic and eastern Pacific rocky shores. This genus comprises different valid species and members of the S. haemastoma complex. In the present study, samples of Stramonita were collected from three different regions of southeastern Brazil. Partial sequences of two mitochondrial genes, COI and 16S rRNA, were used to compare nucleotides sequences between Stramonita specimens. Levels of nucleotide divergence greater than 2% across the three sampled regions were used for differentiation at the species level. One of the identified species was S....
Tipo: Info:eu-repo/semantics/other Palavras-chave: COI; 16s rRNA; Mitochondrial DNA; Southern oyster drill; Brazilian coast.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300392
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Variabilidade genética de duas variedades de tilápia nilótica por meio de marcadores microssatélites PAB
Moreira,Angela Aparecida; Hilsdorf,Alexandre Wagner Silva; Silva,Juliana Viana da; Souza,Vânia Ribeiro de.
O objetivo desse trabalho foi avaliar a variabilidade genética de duas variedades de tilápia nilótica (Oreochromis niloticus), Chitralada e Red Stirling, e de suas progênies submetidas a programas de melhoramento genético, em sistemas intensivos de cultivo por meio de marcadores microssatélites. Foram utilizados 30 animais de cada variedade parental, 30 animais híbridos (CH), provenientes do cruzamento entre as variedades Chitralada e Red Stirling, e 30 animais (RR) provenientes do cruzamento entre os parentais da variedade Red Stirling. Utilizaram-se cinco microssatélites: UNH104, UNH108, UNH118, UNH222 e UNH231. Observaram-se baixos índices de endogamia, com valores de F IS negativo para as duas variedades e seus cruzamentos. Verificou-se diferença...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oreochromis niloticus; Melhoramento genético; Híbridos; Endogamia.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000400010
Registros recuperados: 7
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