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Bacteriophage amplification assay for detection of Listeria spp. using virucidal laser treatment BJM
Oliveira,I.C.; Almeida,R.C.C.; Hofer,E.; Almeida,P.F..
A protocol for the bacteriophage amplification technique was developed for quantitative detection of viable Listeria monocytogenes cells using the A511 listeriophage with plaque formation as the end-point assay. Laser and toluidine blue O (TBO) were employed as selective virucidal treatment for destruction of exogenous bacteriophage. Laser and TBO can bring a total reduction in titer phage (ca. 10(8) pfu/mL) without affecting the viability of L. monocytogenes cells. Artificially inoculated skimmed milk revealed mean populations of the bacteria as low as between 13 cfu/mL (1.11 log cfu/mL), after a 10-h assay duration. Virucidal laser treatment demonstrated better protection of Listeria cells than the other agents previously tested. The protocol was faster...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Listeria; Bacteriophage A511; Laser light; Detection; Skimmed milk.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300040
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Bone marrow fibroblasts in patients with advanced lung cancer BJMBR
Chasseing,N.A.; Hofer,E.; Bordenave,R.H.; Shanley,C.; Rumi,L.S..
In a previous study we demonstrated that the incidence of fibroblast colony-forming units (CFU-F) was very low in bone marrow primary cultures from the majority of untreated advanced non-small lung cancer patients (LCP) compared to normal controls (NC). For this reason, we studied the ability of bone marrow stromal cells to achieve confluence in primary cultures and their proliferative capacity following four continuous subcultures in consecutive untreated LCP and NC. We also evaluated the production of interleukin-1ß (IL-1ß) and prostaglandin E2 (PGE2) by pure fibroblasts. Bone marrow was obtained from 20 LCP and 20 NC. A CFU-F assay was used to investigate the proliferative and confluence capacity. Levels of IL-1ß and PGE2 in conditioned medium (CM) of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fibroblasts; Interleukin-1; Prostaglandin E2; Cancer.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100014
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