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Registros recuperados: 8
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A Multiplex real-time PCR for detection of Mycoplasma gallisepticum and Mycoplasma synoviae in clinical samples from Brazilian commercial poultry flocks BJM
Fraga,Aline Padilha; Vargas,Tatiana de; Ikuta,Nilo; Fonseca,André Salvador Kazantzi; Celmer,Álvaro José; Marques,Edmundo Kanan; Lunge,Vagner Ricardo.
Mycoplasma gallisepticum (MS) and Mycoplasma synoviae (MS) are important avian pathogens and cause economic losses to the poultry industry. Molecular biology techniques are currently used for a rapid detection of these pathogens and the adoption of control measures of the diseases. The aim of this study was to develop and validate a technique for simultaneous detection of MG and MS by multiplex real time polymerase chain reaction (PCR). The complete assay (Multiplex MGMS) was designed with primers and probes specific for each pathogen and developed to be carried out in a single tube reaction. Vaccines, MG and MS isolates and DNA from other Mycoplasma species were used for the development and validation of the method. Further, 78 pooled clinical samples...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Real-time PCR; Mycoplasma gallisepticum; Mycoplasma synoviae.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200028
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Hepatitis C in hemodialysis: the contribution of injection drug use BJID
Galperim,Bruno; Mattos,Angelo A; Stein,Airton T; Schneider,Nuttiane C; Buriol,André; Fonseca,André; Lunge,Vagner; Ikuta,Nilo.
BACKGROUND: Hepatitis C virus (HCV) infection is the most common cause of acute or chronic hepatitis in patients on hemodialysis (HD). The purpose of this study was to describe the prevalence of positive HCV RNA and investigate injection drug use as an emerging risk factor in patients with chronic renal disease on HD. METHODS: This was a multicenter cross-sectional study with 325 patients with chronic renal disease on HD in the period between August 1, 2005 to August 30, 2006, receiving care at four institutions in the city of Porto Alegre, Southern Brazil. Epidemiological data were collected by means of a structured questionnaire. The following laboratory tests were performed: alanine aminotransferase (ALT), anti-hepatitis C virus antibodies (anti-HCV),...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Hepatitis C virus; Hemodialysis; Injection drug use; Risk factors.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000400020
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Human papillomavirus detection and typing using a nested-PCR-RFLP assay BJID
Coser,Janaina; Boeira,Thaís da Rocha; Fonseca,André Salvador Kazantzi; Ikuta,Nilo; Lunge,Vagner Ricardo.
BACKGROUND: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. OBJECTIVES: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to detect and type HPV based on the analysis of L1 gene. METHODS: Analysis of published DNA sequence of mucosal HPV types to select sequences of new primers. Design of an original nested-PCR assay using the new primers pair selected and classical MY09/11 primers. HPV detection and typing in cervical samples using the nested-PCR-RFLP assay. RESULTS: The nested-PCR-RFLP assay detected and typed HPV in cervical samples. Of the total of 128 clinical samples submitted to simple PCR and nested-PCR for detection of HPV,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA probes; HPV polymerase chain reaction polymorphism; Restriction fragment length.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000500009
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Identification of enteric viruses circulating in a dog population with low vaccine coverage BJM
Alves,Christian D.B.T.; Granados,Oscar F.O.; Budaszewski,Renata da F.; Streck,André F.; Weber,Matheus N.; Cibulski,Samuel P.; Pinto,Luciane D.; Ikuta,Nilo; Canal,Cláudio W..
ABSTRACT Although the use of vaccines has controlled enteric diseases in dogs in many developed countries, vaccine coverage is still under optimal situation in Brazil. There is a large population of nonimmunized dogs and few studies about the identification of the viruses associated with diarrhea. To address this situation, stool samples from 325 dogs were analyzed by polymerase chain reaction for the detection of common enteric viruses such as Canine adenovirus (CAdV), Canine coronavirus (CCoV), Canine distemper virus (CDV), Canine rotavirus (CRV) and Carnivorous protoparvovirus 1 (canine parvovirus 2; CPV-2). At least one of these species was detected in 56.6% (184/325) of the samples. The viruses detected most frequently in either diarrheic or...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Distemper; Parvovirus; Diarrhea; Dog; Co-infection.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400790
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Increasing prevalence and dissemination of invasive nontyphoidal Salmonella serotype Typhimurium with multidrug resistance in hospitalized patients from southern Brazil BJID
Reis,Rafael Oliveira dos; Souza,Margarida Neves; Cecconi,Maria Cristina Piccoli; Timm,Loeci; Ikuta,Nilo; Simon,Daniel; Wolf,Jonas Michel; Lunge,Vagner Ricardo.
ABSTRACT Introduction: Nontyphoidal Salmonella serotypes are the main cause of human food-borne infection, including several hospitalization cases in the developing countries. Aim: To detect the main serotypes and to characterize the antibiotic resistance of human non-enteric and enteric nontyphoidal Salmonella from clinical isolates in Brazil. Methods: Salmonella serotypes were identified by microbiological and molecular methods. Susceptibility testing to antibiotics was performed by agar disk diffusion. Real-time PCRs were carried out for the detection of the genus Salmonella as well as serotypes Typhimurium and Enteritidis. Results: A total of 307 nontyphoidal Salmonella were isolated from 289 different patients in a reference laboratory (LACEN-RS)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Antibiotic resistance; Typhimurium; Enteritidis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000500424
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Isolamento de Arcobacter butzleri de músculos de carcaças de suínos de terminação e de matrizes descartadas abatidos em um matadouro no Estado do Rio Grande do Sul, Brasil Ciência Rural
Oliveira,Sérgio José de; Ikuta,Nilo; Lunge,Vagner Ricardo; Fonseca,André; Moraes,Hamilton Luiz; Kuchenbecker,Beatris Sontaz; Passos,Daniel Thompson.
Foram colhidos fragmentos de músculos da carcaça de 74 suínos de abate e de 150 porcas descartadas de granjas de ciclo completo, abatidos em frigorífico no Rio Grande do Sul. Os materiais foram inoculados em meio EMJH (Ellinghausen, MacCullough, Johnson & Harris), incubados a 30ºC e o cultivo foi passado a placas de agar sangue através de membrana de 0,45µm. Foram obtidas 69 amostras de Arcobacter sp, classificadas por PCR como A. butzleri, respectivamente de suínos de abate (21 isolamentos, 29,3%) e matrizes descartadas (48 isolamentos, 32%).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carcaças; Suínos de abate; Matrizes descartadas; Arcobacter butzleri.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000500015
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Isolation of Arcobacter spp from poultry carcasses, in Brazil Ciência Rural
Oliveira,Sérgio José de; Moraes,Hamilton Luiz de Souza; Kuchenbecker,Beatris Sonntag; Ikuta,Nilo; Lunge,Vagner; Fonseca,André; Coiro,José Rafael.
Fourty eight isolates of Arcobacter spp were obtained from 37 poultry carcasses, from abattoir, among 80 carcasses examined. Attempts for culturing were made from the skin and muscle, resulting on 25 positive cultures from muscle and 23 from skin. Classification was achieved by phenotypic characterization and PCR and multiplex PCR, resulting 41 samples of Arcobacter butzleri and 07 Arcobacter sp. This is the first report on the occurrence of Arcobacter in animal carcasses in Brazil.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arcobacter sp; Arcobacter butzleri; Poultry carcasses.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000400013
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Low prevalence of human immunodeficiency virus and hepatitis C virus co-infection in a medium size city in southern Brazil BJID
Librelotto,Carina Sperotto; Simon,Daniel; Ikuta,Nilo; Lunge,Vagner Ricardo.
Tipo: Info:eu-repo/semantics/other
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000600689
Registros recuperados: 8
Primeira ... 1 ... Última
 

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