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| Loureiro y Penha,C.V.; Kubitschek,P.H.B.; Larcher,G.; Perales,J.; Rodriguez León,I.; Lopes-Bezerra,L.M.; Bouchara,J.P.. |
| The incidence of superficial or deep-seated infections due to Candida glabrata has increased markedly, probably because of the low intrinsic susceptibility of this microorganism to azole antifungals and its relatively high propensity to acquire azole resistance. To determine changes in the C. glabrata proteome associated with petite mutations, cytosolic extracts from an azole-resistant petite mutant of C. glabrata induced by exposure to ethidium bromide, and from its azole-susceptible parent isolate were compared by two-dimensional polyacrylamide gel electrophoresis. Proteins of interest were identified by peptide mass fingerprinting or sequence tagging using a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer. Tryptic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida glabrata; Petite mutations; Cytosolic extracts; Proteomic analysis; Azole resistance. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200010 |
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