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Registros recuperados: 28
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Amélioration des productions phytoplanctoniques en écloserie de mollusques : caractérisation des microalgues fourrage ArchiMer
Robert, Rene; Chretiennot-dinet, Marie-josèphe; Kaas, Raymond; Martin-jezequel, Véronique; Moal, Jeanne; Le Coz, Jean-rene; Nicolas, Jean-louis; Bernard, Eudes; Connan, Jean-paul; Le Dean, Loic; Le Gourrierec, Gaetane; Leroy, Bertrand; Quere, Claudie.
in mollusc hatcheries. Consequently, we assessed themost commonly used species to establish their growth and size profiles, their cytomorphological, biochemical, biomolecular identification as well as their cytometer signs, and also their ability to grow under extreme conditions of temperature and salinity. This work aimed at matching the conformity of the strains used in Argenton (dispatched to most of the French commercial hatcheries) to those of a referenced culture collection. A previous screening preceded this study, because of the 45 species and/or clones held originally, Argenton kept no more than 15 species. As most of the latter are also represented in referenced collection, our strains are therefore correctly identified. On the other hand, the...
Tipo: Text Palavras-chave: Ecological limits; Biomolecular profile; Flow cytometry; Biochemical composition; Cytomorphology; Phytoplankton; Mollusc; Hatchery; Ecotolérance; Profil biomoléculaire; Cytométrie en flux; Composition biochimique; Cytomorphologie; Phytoplancton; Mollusques; Ecloserie.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/rapport-1546.pdf
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Approche de l'état physiologique de l'huître creuse Crassostrea gigas au cours d'un cycle saisonnier à Marennes-Oléron ArchiMer
Moal, Jeanne; Samain, Jean-francois; Bodoy, Alain; Le Coz, Jean-rene.
The energy charge was measured for two Crassostrea gigas populations, during an annual survey at Marennes Oleron, to appreciate the condition of health of these oysters. It is assumed that the actual decrease in production of this area is related to a biomass overload which affects the oysters by lowering their scope for growth. Energy charge, an index developped by ATKINSON (1968), defines the energy equilibrium of organisms. It is the adenylic nucleotide ratio: E.C = (ATP + 1/2ADP)/ATP + ADP + AMP). We have measured this parameter to determine the state of health of these oysters. A methodological study has been conducted previously to define the conditions of sampling, conservation and extraction for a HPLC assay. The HPLC method (high performance...
Tipo: Text Palavras-chave: Marennes Oleron; Seasonal variations; Crassostrea gigas; Physiological indice; Nucleotides; Energy charge; Marennes Oléron; Cycle saisonnier; Crassostrea gigas; Indiced physiologique; Nucléotides; Charge énergétique.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/1987/publication-2869.pdf
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Biological characteristics of European hake (Merluccius merluccius) sperm ArchiMer
Groison, Anne-laure; Suquet, Marc; Cosson, Jacky; Le Coz, Jean-rene; Jolivet, Aurelie; Garren, Francois.
Very little is known on European hake's reproductive biology and especially on biological characteristics of its sperm despite a growing interest in its aquaculture potential. This study reports, for the first time some hake sperm characteristics. After activation, the swimming phase lasts 3 min (8 min when activated with 50% sea water (SW) but lower initial velocity). The initial flagellar beat frequency (BF), velocity and percentage of motile cells decreased after 100 s. After 2 days at 4 degrees C, the mean Adenylate Energy Charge (AEC) level was 0.71. Sperm stored at 4 degrees C still showed motility after 9 days for 2 individuals. When cryopreserved, the motility recovery index of thawed spermatozoa ranged from 11.8% to 29.6%.
Tipo: Text Palavras-chave: AEC; Gamete quality; Sperm motility; Sperm Density; Merluccius merluccius.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6553.pdf
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Biological, physiological, immunological and nutritional assessment of farm-reared Litopenaeus stylirostris shrimp affected or unaffected by vibriosis ArchiMer
Mugnier, Chantal; Justou, Carole; Lemonnier, Hugues; Patrois, Jacques; Ansquer, Dominique; Goarant, Cyrille; Le Coz, Jean-rene.
Shrimp aquaculture in New Caledonia is subject to seasonal mortalities during grow-out due to highly virulent Vibrio nigripulchritudo (Vn). To understand the mechanisms affecting shrimp resistance and leading to significant mortality, a shrimp ecophysiology and immunology survey was conducted on two farms, the first considered as a "control" farm (HC), the second affected by the disease (DF). Mortality observed during the survey at DF started 50 days after stocking and was typical of this disease. The main observations regarding shrimp were: (a) growth was not affected by the disease and was faster in the DF than in the HC pond; (b) disease did not affect one sex more than the other, or a specific part of the population in terms of weight; (c) the physical...
Tipo: Text Palavras-chave: Vibriosis; Penaeid shrimp; Field survey; Physiology; Immunology; Nutritional status.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00176/28764/27567.pdf
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Changes in fatty acid and sterol composition during oogenesis in the pearl oyster Pinctada margaritifera ArchiMer
Vahirua Lechat, I; Laure, F; Le Coz, Jean-rene; Bianchini, J; Bellais, M; Le Moullac, Gilles.
The fatty acid and sterol composition of the oyster Pinctada margaritifera during oogenesis and in eggs was analysed. No major differences were observed during oogenesis, but the egg composition was significantly different from that of gonads. The amount of saturated fatty acids was the highest in eggs and the C16:0 predominant (P < 5%); by contrast, the amount of 22:6(n-3) was significantly lower (P < 5%) than in gonads. No major differences were observed for the polar lipid (PL) composition during oogenesis. The main free sterols in gonads and eggs were cholesterol and brassicasterol. Among free sterols, the proportion of cholesterol diminished continuously from the beginning to the end of gonad maturation, and this decrease persisted in eggs after...
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Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-5902.pdf
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Changes in motility, ATP content, morphology and fertilisation capacity during the movement phase of tetraploid Pacific oyster (Crassostrea gigas) sperm ArchiMer
Suquet, Marc; Labbe, C.; Brizard, Raphael; Donval, Anne; Le Coz, Jean-rene; Quere, Claudie; Haffray, P..
Changes in sperm features during the movement phase are especially interesting to study in external fertilization species whose sperm duration movement is long because this implies a significant adaptation of moving cells to the external medium. This study describes the changes in tetraploid Pacific oyster sperm characteristics in relation to time post activation. Sperm individually collected on three tetraploid males were activated in seawater. Their features were analysed over a 24 h period and compared to a sperm pool collected on three diploid males as a reference. The percentage of motile spermatozoa, the intracellular ATP content, and the fine structure of spermatozoa were studied in relation to time post activation. Furthermore, the fertilisation...
Tipo: Text Palavras-chave: Crassostrea gigas; Sperm; Tetraploid; Motility; ATP; Ultrastructure.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00006/11756/8639.pdf
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C/N et contrôle de la physiologie des cultures de phytoplancton ArchiMer
Moal, Jeanne; Samain, Jean-francois; Le Coz, Jean-rene.
Nutrients can be used for induction of carbohydrate or protein metabolism of three unicellular algae, their chemical variations measured by c/N ratio. The C/N determination and evolution can be used as a control for growth phase and satured conditions. Cultures of routine production have been tested by such a method. The results are discussed. [NOT CONTROLLED OCR]
Tipo: Text Palavras-chave: Histoire Ifremer.
Ano: 1979 URL: http://archimer.ifremer.fr/doc/1979/publication-5221.pdf
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Composition en cires, triglycérides, protéines et sucres du mésozooplancton ArchiMer
Alayse-danet, Anne-marie; Daniel, Jean-yves; Le Coz, Jean-rene.
Analyses effectuées sur le zooplancton total des pêches verticales au WP2 et sur des animaux triés à partir des pêches horizontales (Campagne RCA sur les côtes Atlantiques du Portugal). On ne signale pas de différence significative entre la composition biochimique des populations du large et de la côte
Tipo: Text Palavras-chave: Zooplancton; Milieu marin; Composition chimique; Glucide; Cire animale; Triglycéride; Protéine; Océan Atlantique Nord; Upwelling; Méiofaune; Portugal; France; Zone côtière; Zooplankton; Marine environment; Chemical composition; Carbohydrate; Animal wax; Triglyceride; Proteins; North Atlantic; Upwelling; Meiofauna; Portugal; France; Coastal zone.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/00189/29976/28429.pdf
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Compte rendu final du projet Triplofimer : Fiabilité et qualité de la production de naissains triploïdes d'huître creuse (Crassostrea gigas) ArchiMer
Suquet, Marc; Agogue, Julie; Brizard, Raphael; Haffray, Pierrick; Labbe, Catherine; Le Coz, Jean-rene; Pepin, Jean-francois; Rault, Paul; Saulnier, Denis.
Ce rapport fait état d'une étude réalisée au laboratoire de Génétique et Pathologie de l'IFREMER dans le cadre de l'analyse d'épisodes de mortalité anormale survenue en écloserie privée de crevettes pénéides. Plusieurs hypothèses ont été avancées. La présence de Vibrio harveyi a amené à constituer une collection de 40 souches à partir desquelles une identification bactériologique a été effectuée. Elle montre en effet une dominance de souches appartenant à un groupe homogène de Vibrio harveyi. La technique de ribotypage (digestion d'ADN génomique, transfert de Southem-blot et hybridation avec une sonde spécifique) est alors mise en place. Plusieurs difficultés techniques ont ralenti l'analyse et n'ont donc pas permis d'avoir des résultats assez avancés pour...
Tipo: Text Palavras-chave: Huître creuse; Crassostrea gigas; Tétraploïdes; Sperme; Conservation; Diagnostic zoo-sanitaire; Flore bactérienne; Herpès virus..
Ano: 2007 URL: http://archimer.ifremer.fr/doc/00304/41554/40762.pdf
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Ecophysiological and metabolic adaptations to sulphide exposure of the oyster Crassostrea gigas ArchiMer
Le Moullac, Gilles; Cheize, Marie; Gastineau, Olivier; Daniel, Jean-yves; Le Coz, Jean-rene; Huvet, Arnaud; Moal, Jeanne; Pouvreau, Stephane; Van Wormhoudt, A; Samain, Jean-francois.
The purpose of this study is to examine the response of the oyster Crassostrea gigas to sulphide at different biological scales. A first experiment was designed to measure for 20 h the clearance (CR) and oxygen consumption (OC) rates of oysters exposed at different concentrations of sulphide (0, 3, 6, 12, 20, and 40 mu M). The second experiment was carried out to evaluate the metabolic adaptations to chronic sulphide exposure for 10 days by measuring PK and PEPCK enzyme activities, succinate and alanine content, the adenylate energy charge (AEC), and the activity of the electron transport system (ETS)in whole oysters at above 20 mu M. PK and PEPCK mRNAs and enzyme activities have been measured in adductor muscle. When exposed to sulphide above 20 mu M, CR...
Tipo: Text Palavras-chave: ATP; AEC; Electron transport system; Succcinate; Alanine; Phosphoenolpyruvate carboxykinase; Pyruvate kinase; Oxygen consumption; Clearance rate; Crassostrea gigas; Sulphide; Oyster.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3924.pdf
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Effect of reproduction on escape responses, metabolic rates and muscle mitochondrial properties in the scallop Placopecten magellanicus ArchiMer
Kraffe, E; Tremblay, R; Belvin, S; Le Coz, Jean-rene; Marty, Y; Guderley, H.
In scallops, gametogenesis and spawning can diminish the metabolic capacities of the adductor muscle and reduce escape response performance. To evaluate potential mechanisms underlying this compromise between reproductive investment and escape response, we examined the impact of reproductive stage (pre-spawned, spawned and reproductive quiescent) of the giant scallop, Placopecten magellanicus, on behavioural (i.e., escape responses), physiological (i.e., standard metabolic rates and metabolic rates after complete fatigue) and mitochondrial capacities (i.e., oxidative rates) and composition. Escape responses changed markedly with reproductive investment, with spawned scallops making fewer claps and having shorter responses than pre-spawned or...
Tipo: Text Palavras-chave: Placopecten magellanicus; Sterols; Fatty acids; Plasmalogens; Phospholipids; Cytochrome C oxidase; Metabolic rate; Escape response; Muscle; Scallops; Reproduction; Mitochondria.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4786.pdf
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Effect of sediment nearness on the metabolic enzyme activity and energy state of the oyster Crassostrea gigas ArchiMer
Le Moullac, Gilles; Fleury, Pierre-gildas; Le Coz, Jean-rene; Moal, Jeanne; Samain, Jean-francois.
This study was designed to assess in situ the effects of sediment nearness on Crassostrea gigas metabolism. One year-old oysters were reared for 5 months (April-August) in plastic bags on metallic frames at 10 and 60 cm off the sediment at the beginning of April. The management of the energetic resources (storage, consumption) was estimated with respect to biochemical changes of proteins, lipids and carbohydrates. In order to evaluate the effect of the sediment nearness at cellular level, the metabolic rate was evaluated by assessing pyruvate kinase (PK) and phosphoenolpyruvate carboxykinase (PEPCK) enzyme activities and electron transport system (ETS) activity. The metabolic pathways were assessed by measuring ATP and intermediary metabolites such as...
Tipo: Text Palavras-chave: Aspartate; Succinate; Alanine; ETS; PEPCK; PK; Carbohydrate; Lipids; Proteins; Sediment; Oyster; Aspartate; Succinate; Alanine; ETS; PEPCK; PK; Glucides; Lipides; Proteines; Sédiment; Huître.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2803.pdf
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Effect of the culture system and culture technique on biochemical characteristics of Pavlova lutheri and its nutritional value for Crassostrea gigas larvae ArchiMer
Ponis, Emanuele; Parisi, G; Le Coz, Jean-rene; Robert, Rene; Zittelli, G; Tredici, R.
Pavlova lutheri was cultivated in carboys using batch technique and in a flat alveolar photobioreactor (FAP), using initially batch and then semi-continuous techniques. Growth and productivity of the cultures were compared and their nutritional quality was assessed by analysing their biochemical composition (gross composition, fatty acids and sterols) and by evaluating their food value for Pacific oyster (Crassostrea gigas) larvae as bispecific diet in association with Chaetoceros calcitrans forma pumilum. The highest productivity was achieved with the FAP operating in semi-continuous mode. Gross composition of P. lutheri was similar in all cultures, while cellular organic matter content of the microalga grown in FAP in semi-continuous mode was lower....
Tipo: Text Palavras-chave: Semi continuous culture; Pavlova lutheri; Flat alveolar photobioreactors; Crassostrea gigas; Carboys; Biochemical composition; Batch culture.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-6117.pdf
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Effet de la qualité de l'eau sur la croissance larvaire de Pecten maximus en écloserie : observations préliminaires ArchiMer
Samain, Jean-francois; Cochard, Jean-claude; Chevolot, Lionel; Daniel, Jean-yves; Jeanthon, C.; Le Coz, Jean-rene; Marty, Y.; Moal, Jeanne; Prieur, D.; Salaun, Michele.
Some positive growth differences can be observed, all year long, in Pecten maximus larvae cultivated in an old fishpond, compared with the water which flows into it periodically. These waters are filtered at 1 µm through CUNO cartridges. Moreover, the larvae are cultivated in the same light, temperature and food (cultured phytoplankton) conditions. This difference is significant in terms of larvae size, dry weight, organic matter, ashes, total proteins, glycogen, and lipids. A series of preliminary observations on the chemical composition of these two waters after filtration (amino acids, fatty acids, organic mater, nutritive salts), on the bacterial flora (total, heterotrophic organisms, vibrio), on the particular material using electron microscopy, on...
Tipo: Text Palavras-chave: Larve; Ecloserie; Pecten maximus; Facteur croissance; Qualité eau.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/1986/acte-6185.PDF
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Enhancement of neutral lipid productivity in the microalga Isochrysis affinis Galbana (T-Iso) by a mutation-selection procedure ArchiMer
Bougaran, Gael; Rouxel, Catherine; Dubois, Nolwenn; Kaas, Raymond; Grouas, Sophie; Lukomska, Ewa; Le Coz, Jean-rene; Cadoret, Jean-paul.
Microalgae offer a high potential for energetic lipid storage as well as high growth rates. They are therefore considered promising candidates for biofuel production, with the selection of high lipid-producing strains a major objective in projects on the development of this technology. We developed a mutation-selection method aimed at increasing microalgae neutral lipid productivity. A two step method, based on UVc irradiation followed by flow cytometry selection, was applied to a set of strains that had an initial high lipid content and improvement was assessed by means of Nile-red fluorescence measurements. The method was first tested on Isochrysis affinis galbana (T-Iso). (...)
Tipo: Text Palavras-chave: Strain selection; Isochrysis; Neutral lipid; Ultra violet; Flux cytometry; Biofuel.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00085/19643/17418.pdf
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Genetic and reproduction strategy in Crassostrea gigas mortality ArchiMer
Moal, Jeanne; Bedier, Edouard; Fleury, Pierre-gildas; Langlade, Aime; Le Coguic, Yvette; Degremont, Lionel; Boudry, Pierre; Le Coz, Jean-rene; Pouvreau, Stephane; Enriquez-diaz, M.; Lambert, Christophe; Quere, Christele; Soudant, Philippe; Samain, Jean-francois.
Bi-parental families were produced in hatchery and tested in the field in 2001. Two sets of 5 families were constituted, selected on their high (R) and low (S) survival. These two sets were reared in Brittany from March to November 2002. Samplings were performed twice a month to obtain data on biometry, survival, reproductive cycle, biochemical composition, adenylate energy charge, hemolymph parameters (ions and defense system) and muscle strength. "R" and "S" oysters exhibited different reproductive effort and spawning strategy. "R" oysters allocated less energy in gonad than "S" ones and presented a complete spawning at the end of August contrary to the "S" which spawns partially. Mortality started in July when the seawater temperature reached 19 degree...
Tipo: Text Palavras-chave: Summer; Reproduction; Oyster culture; Mortality; Genetic diversity; Commercial species; Breeding.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-3345.pdf
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Influence of phytoplankton diet mixtures on microalgae consumption, larval development and settlement of the Pacific oyster Crassostrea gigas (Thunberg) ArchiMer
Rico Villa, Benjamin; Le Coz, Jean-rene; Mingant, Christian; Robert, Rene.
Microalgae commonly used as feed for bivalves, Pavlova lutheri (P), Isochrisis affinis galbana (T) and Chaetoceros calcitrans forma pumilum (Cp), were fed to Pacific oyster Crassostrea gigas to assess their nutritional value for larval development and metamorphosis during two experiments. Monospecific, bispecific and trispecific diets were firstly evaluated during 3 weeks from D larvae to young postlarvae. Then bispecific diets, based on different T and Cp proportions, were assessed during a similar period. Concurrently, ingestion was studied through the whole larval and postlarval development for each diet and/or diet mixture. Because lipids are assumed to be a key nutrient for bivalves, biochemical analysis was undertaken on the second set of trials...
Tipo: Text Palavras-chave: Lipids; Grazing; Metamorphosis; Growth; Larvae; Crassostrea gigas.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1707.pdf
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Lipid composition of the pacific lion-paw scallop, Nodipecten subnodosus, in relation to gametogenesis - 2. Lipid classes and sterols ArchiMer
Palacios, Elena; Racotta, I.s.; Arjona, O; Marty, Y; Le Coz, Jean-rene; Moal, Jeanne; Samain, Jean-francois.
Knowledge on the energy distribution during the reproductive cycle of the giant lion-paw scallop, Nodipecten subnodosus is limited, but necessary for aquaculture purposes. In a previous work, we found that natural food availability can sustain the full cost of gametogenesis in optimal environmental conditions. The present study analyses the lipid and sterol classes' composition of several tissues during gametogenesis. In female gonads, the quantity of triacylglycerol (TG) increased parallel to gametogenesis from December (spawned gonads, 0.7 mg g(-1) or 7% of total lipids) to June (ripe gonads, 52 mg g(-1) or 66% of total lipids). In male gonads sampled in June, the concentration of TG (0.7 mg g(-1) or 3.5% of total lipids) and esterified sterols was...
Tipo: Text Palavras-chave: Sterol; Reproduction; Phospholipid; Mollusks; Acylglycerides.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2635.pdf
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Metabolic adjustments in the oyster Crassostrea gigas according to oxygen level and temperature ArchiMer
Le Moullac, Gilles; Queau, Isabelle; Le Souchu, Pierrick; Pouvreau, Stephane; Moal, Jeanne; Le Coz, Jean-rene; Samain, Jean-francois.
The purpose of this study was to examine the responses of the oyster C. gigas to oxygen levels at subcellular and whole-organism levels. Two experiments were carried out. The first experiment was designed to measure at 15°C, 20°C and 25°C for 20 hours the clearance (CR) and oxygen consumption (OC) rates of oysters exposed at different concentrations of oxygen. The goal of this first part was to estimate the hypoxic threshold for oysters below which their metabolism shifts toward anaerobiosis, by estimating the oxygen critical point (PcO2) at 15, 20 and 25°C. The second experiment was carried out to evaluate the metabolic adaptations to hypoxia for 20 days at three temperatures: 12°C, 15°C and 20°C. The metabolic pathways were characterized by the...
Tipo: Text Palavras-chave: Succinate; Alanine; Phosphoenolpyruvate carboxykinase; Pyruvate kinase; Temperature; Hypoxia; Oysters.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2693.pdf
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Modèle de détection rapide des effets sublétaux des polluants. II. Un exemple in situ : anomalie métabolique de copépode hyponeustonique Anomalocera patersoni en coïncidence avec une marée noire ArchiMer
Samain, Jean-francois; Moal, Jeanne; Alayse-danet, Anne-marie; Daniel, Jean-yves; Le Coz, Jean-rene.
Model for Rapid Detection of Sublethal Effects of Pollutants. II. An in situ Example: Anomalous Metabolism of the Hyponeustonic Copepod Anomalocera patersoni Coinciding with an Oil Spill An unusual correlation has been obsemed between the amylase and trypsin content of a natural zooplankton population and of the hyponeustonic copepod Anomalocera patersoni and the oil spill of the "Amoco Cadiz" in 1978. This anomaly has also been obsewed in Artemia sp. cultures (San Francisco Bay strain) poisoned by copper. This phenomenon could originate from a general physiological perturbation. Such a non-specific perturbation could explain the anormal correlation between parameters which are usually independent and specifically regulated. The determination of such...
Tipo: Text Palavras-chave: Histoire Ifremer.
Ano: 1981 URL: http://archimer.ifremer.fr/doc/1981/publication-5362.pdf
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