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Chromosomal organization of simple sequence repeats in the Pacific oyster (Crassostrea gigas): (GGAT)(4), (GT)(7) and (TA)(10) chromosome patterns ArchiMer
Bouilly, Karine; Chaves, R.; Leitao, A.; Benabdelmouna, Abdellah; Guedes Pinto, H..
Chromosome identification is essential in oyster genomic research. Fluorescence in situ hybridization (FISH) offers new opportunities for the identification of oyster chromosomes. It has been used to locate satellite DNAs, telomeres or ribosomal DNA sequences. However, regarding chromosome identification, no study has been conducted with simple sequence repeats (SSRs). FISH was used to probe the physical organization of three particular SSRs, (GGAT)(4), (GT)(7) and (TA)(10) onto metaphase chromosomes of the Pacific oyster, Crassostrea gigas. Hybridization signals were observed in all the SSR probes, but the distribution and intensity of signals varied according to the oligonucleotide repeat. The intercalary, centromeric and telomeric bands were observed...
Tipo: Text Palavras-chave: Simple sequence repeats (SSRs); Mollusks; Fluorescence in situ hybridization (FISH); Crassostrea gigas; Chromosome identification.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6132.pdf
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Karyotype variation in neoplastic cells associated to severity of disseminated neoplasia in the cockle Cerastoderma edule ArchiMer
Matias, A. M.; Diaz, S.; Teixeira De Sousa, Joana; Villalba, A.; Carballal, M. J.; Leitao, A..
Disseminated neoplasia (DN) is characterized by an abnormal cell proliferation, usually resulting in death of the affected mollusks. In the present study we performed, for the first time, karyotypes of two different stages of DN in the cockle Cerastoderma edule. Analysis of moderate (N2) and high (N3) severity neoplasic metaphases displayed a chromosome number ranging from 57 to 317, instead of the normal diploid number of 38. Cockles with N2 showed a lower percentage of microchromosomes and a higher ploidy than those with N3. The comparison of both cytogenetic profiles contributes to better understand the evolution of DN in cockles.
Tipo: Text Palavras-chave: Karyotype; Disseminated neoplasia; Cerastoderma edule; Microchromosomes.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00184/29550/27876.pdf
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