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Assessment of genotoxicity and cytotoxicity of "lixeira" (Curatella americanaL.) λ using the prophage induction test (SOS inductest) BJPS
Vilar,Juliana Brandstetter; Andrade,Laryssa Silva de; Leite,Kaio Ramos; Ferreira,Heleno Dias; Chen,Lee Chen.
Curatella americana L., commonly known as "lixeira" in Brazil, has been used in folk medicine to treat ulcers and inflammations. The purpose of the present work was to evaluate the cytotoxic and genotoxic potential of the ethanolic extract of C. americana stem bark using the prophage λ induction test (SOS inductest). To evaluate the cytotoxicity of this plant, after treatment with different concentrations of the extract, Escherichia coli WP2s(λ) cultures were diluted in M9 buffer, inoculated into LB plates, and incubated for 24 h at 37 °C. To assess genotoxicity, the lysogenic strain E. coli WP2s(λ) was treated with different concentrations of the extract. Then, the lysogenic strain was added to the indicator strain (RJF013), LB(1/2)(malt/amp), seeded into...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Curatella americana; Lixeira; Cytotoxicity; Genotoxicity; Lysogenic induction.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300015
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