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Regeneration of soybean via embryogenic suspension culture Scientia Agricola
Droste,Annette; Leite,Paulo César Pimentel; Pasquali,Giancarlo; Mundstock,Elsa Cristina de; Bodanese-Zanettini,Maria Helena.
In an attempt to establish an alternative plant regeneration system for soybean [Glycine max (L.) Merrill] cultivars used in Brazilian breeding programs, ten genotypes were tested for their embryogenic potential. Cotyledons were removed as explants from immature seeds harvested from field-grown plants. After 45 days on induction medium, the number of responding cotyledons and the number of somatic embryos per immature cotyledon were evaluated. The percentage of explants that produced somatic embryos varied from 1 to 70% among cultivars. The average number of somatic embryos produced per cotyledon pair ranged from 0.01 to 10.3 with a mean of 3.4. Suspension cultures were initiated with three Agrobacterium tumefaciens susceptible cultivars. Suspensions were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Somatic embryogenesis; Liquid medium; Plant tissue culture.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162001000400017
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