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| Registros recuperados: 19 | |
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| OKINO, C. H.; MALAGO JUNIOR, W.; MARCONDES, C. R.; GIGLIOTI, R.; MONTASSIER, H. J.; OLIVEIRA, H. N. DE; OLIVEIRA, M. C. de S.. |
| Two mutations in the CD4 bovine gene (G>T/Q306H; A>C/K310N) were identified as causative for altered staining with anti-CD4 mAb #CC8. We developed a HRM qPCR for genotyping these mutations and compare with immunophenotyping in different cattle breeds. The assay distinguished five genotypes, B (homozygous, G/A) and C (heterozygous, G/A and T/C), found in taurine, A (homozygous, G/C) and D (heterozygous, T/C and G/C), found in zebu. The E genotype (homozygous, T/C) was not observed in tested animals. As expected, B and C presented high/very high and intermediate CD4 staining, respectively. The lack/low CD4 staining was mainly related to the A, while the intermediate staining was mainly related to D genotype. The developed HRM qPCR assay accurately... |
| Tipo: Artigo de periódico |
Palavras-chave: QPCR; High Resolution Melt; Immunophenotyping; T helper cells; Genotype. |
| Ano: 2022 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1144902 |
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| MATTOS, G. L. M.; OKINO, C. H.; TREVISOL, I. M.; MORES, M. A. Z.; BRENTANO, L.; ESTEVES, P. A.; LOPES, L. dos S.; BASTOS, A. P. de A.; CARON, L.; FILHO, T. F.; DUARTE, I.; MONTASSIER, H. J.. |
| Tipo: Separatas |
Palavras-chave: Cellular imune response.; Avicultura; Virologia; Infectious bronchitis.. |
| Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1014170 |
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| LOPES, P. D.; OKINO, C. H.; FERNANDO, F. S.; PAVANI, C.; MARIGUELA, V. C.; MONTASSIER, M. DE F. S.; MONTASSIER, H. J.. |
| Efficient vaccines are the main strategy to control the avian coronavirus (AvCoV), although several drawbacks related to traditional attenuated and inactivated vaccines have been reported. These counterpoints highlight the importance of developing new alternative vaccines against AvCoV, especially those able to induce long-lasting immune responses. This study evaluated and compared two inactivated vaccines formulated with AvCoV BR-I variants, one composed of chitosan nanoparticles (AvCoV-CS) and the second by Montanide oily adjuvant (AvCoV-O). Both developed vaccines were administered in a single dose or associated with the traditional Mass attenuated vaccine. The AvCoV-CS vaccine administered alone or associated with the Mass vaccine was able to induce... |
| Tipo: Artigo de periódico |
Palavras-chave: Avian infectious bronchitis; Cellular immune response; Delivery carrier adjuvant; Mucosal immunization; Oil adjuvant. |
| Ano: 2021 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137397 |
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| OKINO, C. H.; MORES, M. A. Z.; MATTOS, G. L. M.; ESTEVES, P. A.; BRENTANO, L.; MONTASSIER, H. J.; TREVISOL, I. M.. |
| A Bronquite Infecciosa das galinhas (BI), causada pelo vírus da BI (VBI), possui ocorrência mundial e é responsável por perdas de milhões de dólares anuais na produção de frangos, matrizes e postura. A replicação primária do VBI ocorre no epitélio traqueal, ocasionando uma série de alterações patológicas. Entretanto, os mecanismos envolvidos localmente na resposta imune e patogênese da BI permanecem não elucidados completamente. O objetivo desse trabalho foi o de avaliar comparativamente o perfil de expressão de genes envolvidos na resposta imune na traqueia de aves infectadas experimentalmente com isolados de campo brasileiros do VBI genotipados como ?variantes?. |
| Tipo: Separatas |
Palavras-chave: Ave.; Doença animal; Bronquite.; Chickens; Respiratory tract diseases; Bronchitis. |
| Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1014149 |
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| OKINO, C. H.; MORES, M. A. Z.; TREVISOL, I. M.; COLDEBELLA, A.; MONTASSIER, H. J.; BRENTANO, L.. |
| Avian infectious bronchitis virus (IBV) primarily replicates in epithelial cells of the upper respiratory tract of chickens, inducing both morphological and immune modulatory changes. However, the association between the local immune responses induced by IBV and the mechanisms of pathogenesis has not yet been completely elucidated. This study compared the expression profile of genes related to immune responses in tracheal samples after challenge with two Brazilian field isolates (A and B) of IBV from the same genotype, associating these responses with viral replication and with pathological changes in trachea and kidney. We detected a suppressive effect on the early activation of TLR7 pathway, followed by lower expression levels of inflammatory related... |
| Tipo: Artigo de periódico |
Palavras-chave: Bronquite; Frango; Bronchitis; Infectious bronchitis; Infectious bronchitis virus; Chickens. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094609 |
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| ROCHA, T. G.; SILVA, K. R.; SILVA, F. D. F.; ALFERI, A. A.; SILVA, D. G.; MONTASSIER, H. J.; BUZINARO, M. da G.; GREGORI, F.; ZAFALON, L. F.; FAGLIARI, J. J.. |
| The main strategy to prevent bovine rotavirus group A (RVA) diarrhea in calves is to vaccinate late-term dams aiming to enhance passive immunity transfer of specific immunoglobulins against the virus. This study aimed to evaluate influence of parity in titers of immunoglobulin G (IgG), IgG1 and IgM in serum and colostrum of vaccinated or unvaccinated Holstein cows and in serum of its calves, associated with monitoring for RVA diarrhea in calves. Cows and its calves were allotted into groups according to parity and vaccination (primiparous/multiparous; vaccinated/unvaccinated) and serum and colostrum samples of cows were taken as well as serum and fecal samples of its calves. Parturition influenced colostral titers of IgG and IgG1, which were higher in... |
| Tipo: Artigo de periódico |
Palavras-chave: Bovine group A rotavirus; Humoral immune response; Dairy calves; Diarrhea; Vaccination. |
| Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127571 |
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| SANTOS, R. M. dos; FERNANDO, F. S.; MONTASSIER, M. de F. S.; SILVA, K. R.; LOEPS, P. D.; PAVANI, C.; BORZI, M. M.; OKINO, C. H.; MONTASSIER, H. J.. |
| In this study, we evaluated antibody and cell-mediated immune (CMI) responses in the mucosal and systemic compartments and protection against challenge with a nephropathogenic Brazilian (BR-I) strain of infectious bronchitis virus (IBV) in chickens submitted to a vaccination regime comprising a priming dose of heterologous live attenuated Massachusetts vaccine followed by a booster dose of an experimental homologous inactivated vaccine two weeks later. This immunization protocol elicited significant increases in serum and lachrymal levels of anti-IBV IgG antibodies and upregulated the expression of CMI response genes, such as those encoding CD8? chain and Granzyme homolog A in tracheal and kidney tissues at 3, 7, and 11 days post-infection in the... |
| Tipo: Artigo de periódico |
Palavras-chave: Cell mediated immunity; Oil adjuvant; Avian coronavirus; Humoral immunity; Mucosal immunity. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1111878 |
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| Registros recuperados: 19 | |
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