Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 18
Primeira ... 1 ... Última
Imagem não selecionada

Imprime registro no formato completo
Analysis of expressed sequence tags from Citrus sinensis L. Osbeck infected with Xylella fastidiosa Genet. Mol. Biol.
Souza,Alessandra A. de; Takita,Marco A.; Coletta-Filho,Helvécio D.; Targon,Maria Luisa P.N.; Carlos,Eduardo F.; Locali-Fabris,Eliane C.; Amaral,Alexandre M.; Freitas-Astúa,Juliana; Silva-Pinhati,Ana Carla O.; Boscariol-Camargo,Raquel L.; Berger,Irving J.; Rodrigues,Carolina M.; Reis,Marcelo S.; Machado,Marcos A..
In order to understand the genetic responses resulting from physiological changes that occur in plants displaying citrus variegated chlorosis (CVC) symptoms, we adopted a strategy of comparing two EST libraries from sweet orange [Citrus sinensis (L.) Osbeck]. One of them was prepared with plants showing typical CVC symptoms caused by Xylella fastidiosa and the other with non-inoculated plants. We obtained 15,944 ESTs by sequencing the two cDNA libraries. Using an in silico hybridization strategy, 37 genes were found to have significant variation at the transcriptional level. Within this subset, 21 were up-regulated and 16 were down-regulated in plants with CVC. The main functional categories of the down-regulated transcripts in plants with CVC were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sweet orange; EST; CVC; Compatible interaction.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500023
Imagem não selecionada

Imprime registro no formato completo
Avaliação in vitro de genótipos de citros a Phytophthora parasitica Trop. Plant Pathol.
Siviero,Amauri; Santos,Francineide A.; Cristofani,Mariângela; Furtado,Edson L.; Machado,Marcos A..
Este trabalho teve como objetivo avaliar in vitro a reação de porta-enxertos de citros (Citrus spp.) a Phytophthora parasitica. As plântulas foram cultivadas em meio MS por 40 dias sendo, submetidas a fotoperíodo de 18 h, à temperatura de 25 ºC. A inoculação foi realizada através da inserção de agulha infestada com micélio de P. parasitica. A avaliação foi realizada aos 15 dias após a inoculação, medindo-se o comprimento das lesões em centímetros. O delineamento experimental foi inteiramente casualizado, com 15 repetições. Os resultados estão de acordo com as reações de campo dos genótipos e podem ser de grande utilidade em trabalhos envolvendo resistência varietal e seleção precoce de plantas.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Citros; Resistência a doenças; Seleção precoce.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000300010
Imagem não selecionada

Imprime registro no formato completo
Bioinformatics for the Citrus EST Project (CitEST) Genet. Mol. Biol.
Reis,Marcelo S.; Takita,Marco A.; Palmieri,Darío A.; Machado,Marcos A..
In this work we describe all the computational environments, pipelines, and web services developed for the CitEST transcriptome project, on which all the annotation researchers relied. We also present a complete list of CitEST libraries and, for each of them, the general features after the in silico processing, showing some quantitative information.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Transcriptomics; Comparative genomics; Clustering; Trimming; Digital northern.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500031
Imagem não selecionada

Imprime registro no formato completo
Breeding, genetic and genomic of citrus for disease resistance Rev. Bras. Frutic.
Machado,Marcos A.; Cristofani-Yaly,Mariângela; Bastianel,Marinês.
Although the citriculture is one of the most important economic activities in Brazil, it is based on a small number of varieties. This fact has contributed for the vulnerability of the culture regarding the phytosanitary problems. A higher number of varieties/genotypes with potential for commercial growing, either for the industry or fresh market, has been one of the main objectives of citrus breeding programs. The genetic breeding of citrus has improved, in the last decades, due to the possibility of an association between biotechnological tools and classical methods of breeding. The use of molecular markers for early selection of zygotic seedlings from controlled crosses resulted in the possibility of selection of a high number of new combination and, as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citriculture; Improvement; Hybrids.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452011000500019
Imagem não selecionada

Imprime registro no formato completo
Comparative analysis of differentially expressed sequence tags of sweet orange and mandarin infected with Xylella fastidiosa Genet. Mol. Biol.
Souza,Alessandra A. de; Takita,Marco A.; Coletta-Filho,Helvécio D.; Campos,Magnólia A.; Teixeira,Juliana E.C.; Targon,Maria Luísa P.N.; Carlos,Eduardo F.; Ravasi,Juliano F.; Fischer,Carlos N.; Machado,Marcos A..
The Citrus ESTs Sequencing Project (CitEST) conducted at Centro APTA Citros Sylvio Moreira/IAC has identified and catalogued ESTs representing a set of citrus genes expressed under relevant stress responses, including diseases such as citrus variegated chlorosis (CVC), caused by Xylella fastidiosa. All sweet orange (Citrus sinensis L. Osb.) varieties are susceptible to X. fastidiosa. On the other hand, mandarins (C. reticulata Blanco) are considered tolerant or resistant to the disease, although the bacterium can be sporadically detected within the trees, but no disease symptoms or economic losses are observed. To study their genetic responses to the presence of X. fastidiosa, we have compared EST libraries of leaf tissue of sweet orange Pêra IAC (highly...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ESTs; Citrus variegated chlorosis; Biotic stress; CitEST; Phytopathogen.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500024
Imagem não selecionada

Imprime registro no formato completo
Development and characterization of polymorphic microsatellite markers for the sweet orange (Citrus sinensis L. Osbeck) Genet. Mol. Biol.
Novelli,Valdenice M.; Cristofani,Mariangela; Souza,Alessandra A.; Machado,Marcos A..
The aims of this study were to develop simple sequence repeat markers (SSRs or microsatellite markers) in citrus and to evaluate the efficiency of these markers for characterization of sweet orange. We developed SSRs from a genomic library of 'Pêra IAC' sweet orange enriched for AG/TC, GT/CA, TCA/AGT and AAC/TTG sequence repeats. We selected 279 sequences from which 171 primer pairs were designed of which 113 with the best banding patterns were selected. Characterization of sweet orange microsatellite loci revealed that AG/TC was the most abundant (69%) microsatellite class isolated, followed by GT/CA (15.9%), TCA/AGT (8%) and AAC/TTG (6.2%). The number of alleles ranged from 1 to 4, with a mean of 2 alleles per locus. Four microsatellite loci developed in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus species; Germplasm bank; Polymorphism; Molecular markers.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100018
Imagem não selecionada

Imprime registro no formato completo
Differential expression of genes identified from Poncirus trifoliata tissue inoculated with CTV through EST analysis and in silico hybridization Genet. Mol. Biol.
Cristofani-Yaly,Mariângela; Berger,Irving J.; Targon,Maria Luisa P.N.; Takita,Marco A.; Dorta,Sílvia de O.; Freitas-Astúa,Juliana; Souza,Alessandra A. de; Boscariol-Camargo,Raquel L.; Reis,Marcelo S.; Machado,Marcos A..
Citrus is the most important fruit crop in Brazil and Citrus tristeza virus (CTV) is considered one of the most important pathogens of citrus. Most citrus species and varieties are susceptible to CTV infection. However, Poncirus trifoliata, a close relative of citrus, is resistant to the virus. In order to better understand the responses of citrus plants to the infection of CTV, we constructed expressed sequence tag (EST) libraries with tissues collected from Poncirus trifoliata plants, inoculated or not with Citrus tristeza virus at 90 days after inoculation, grafted on Rangpur lime rootstocks. We generated 17,867 sequence tags from Poncirus trifoliata inoculated (8,926 reads) and not (8,941 reads) with a severe CTV isolate. A total of 2,782 TCs...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus; Disease resistance; Citrus tristeza virus; Biotic stress.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500025
Imagem não selecionada

Imprime registro no formato completo
Differentially expressed stress-related genes in the compatible citrus-Citrus leprosis virus interaction Genet. Mol. Biol.
Freitas-Astúa,Juliana; Bastianel,Marinês; Locali-Fabris,Eliane C.; Novelli,Valdenice M.; Silva-Pinhati,Ana Carla; Basílio-Palmieri,Ana Carolina; Targon,Maria Luisa P.N.; Machado,Marcos A..
Leprosis, caused by Citrus leprosis virus, cytoplasmic type (CiLV-C), is the main viral disease in the Brazilian citrus industry. This occurs because of the widespread source of inoculum and the year-round presence of the vector, the tenuipalpid mite Brevipalpus phoenicis, in citrus plants. In addition, while some Citrus species are resistant to CiLV-C, C. sinensis, the main cultivated species in the country, is extremely susceptible to the disease. The main objective of this work was to identify genes in C. sinensis cv. Pêra plants that were differentially expressed after the host was challenged with CiLV-C. In order to accomplish that, cDNA libraries were constructed from healthy and CiLV-inoculated sweet orange leaves. Two hundred and fifty-four genes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: EST; Disease; CiLV; In silico hybridization; Sweet orange; Citrus sinensis.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500026
Imagem não selecionada

Imprime registro no formato completo
Flutuação populacional de cigarrinhas vetoras de Xylella fastidiosa Wells et al. em pomares comerciais de citros no noroeste do Paraná Neotropical Entomology
Nunes,William M.C.; Molina,Rúbia de O.; Albuquerque,Fernando A. de; Corazza-Nunes,Maria J.; Zanutto,Carlos A.; Machado,Marcos A..
A Clorose Variegada dos Citros (CVC) é uma importante doença causada pela bactéria Xylella fastidiosa Wells et al., que é transmitida por insetos sugadores do xilema como vetores, denominados cigarrinhas (Hemiptera: Cicadellidae). No presente estudo, avaliou-se a flutuação populacional de espécies de cigarrinhas pertencentes às tribos Cicadellini e Proconiini, da subfamília Cicadelinae, em um pomar comercial de laranja-doce [Citrus sinensis (L.) Osb.], contendo as variedades Valência, Natal, Pêra e Folha Murcha, localizados no Noroeste do Paraná. Amostragens quinzenais foram realizadas com o uso de armadilhas adesivas amarelas, no total de 24 armadilhas em cada avaliação, de novembro de 1999 a março de 2004. As espécies mais representativas foram...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CVC; Citrus sinensis; Inseto vetor; Cicadellidae.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2007000200012
Imagem não selecionada

Imprime registro no formato completo
Identification and in silico analysis of the Citrus HSP70 molecular chaperone gene family Genet. Mol. Biol.
Fietto,Luciano G.; Costa,Maximiller D.L.; Cruz,Cosme D.; Souza,Alessandra A.; Machado,Marcos A.; Fontes,Elizabeth P.B..
The completion of the genome sequencing of the Arabidopsis thaliana model system provided a powerful molecular tool for comparative analysis of gene families present in the genome of economically relevant plant species. In this investigation, we used the sequences of the Arabidopsis Hsp70 gene family to identify and annotate the Citrus Hsp70 genes represented in the CitEST database. Based on sequence comparison analysis, we identified 18 clusters that were further divided into 5 subgroups encoding four mitochondrial mtHsp70s, three plastid csHsp70s, one ER luminal Hsp70 BiP, two HSP110/SSE-related proteins and eight cytosolic Hsp/Hsc70s. We also analyzed the expression profile by digital Northern of each Hsp70 transcript in different organs and in response...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chaperones; Digital northern; Heat shock proteins; Genome; Expressed sequence tags.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500017
Imagem não selecionada

Imprime registro no formato completo
In silico analysis of ESTs from roots of Rangpur lime (Citrus limonia Osbeck) under water stress Genet. Mol. Biol.
Boscariol-Camargo,Raquel L.; Berger,Irving J.; Souza,Alessandra A.; Amaral,Alexandre M. do; Carlos,Eduardo F.; Freitas-Astúa,Juliana; Takita,Marco A.; Targon,Maria Luisa P.N.; Medina,Camilo L.; Reis,Marcelo S.; Machado,Marcos A..
CitEST project resulted in the construction of cDNA libraries from different Citrus sp. tissues under various physiological conditions. Among them, plantlets of Rangpur lime were exposed to hydroponic conditions with and without water stress using PEG6000. RNA from roots was obtained and generated a total of 4,130 valid cDNA reads, with 2,020 from the non-stressed condition and 2,110 from the stressed set. Bioinformatic analyses measured the frequency of each read in the libraries and yielded an in silico transcriptional profile for each condition. A total of 40 contigs were differentially expressed and allowed to detect up-regulated homologue sequences to well known genes involved in stress response, such as aquaporins, dehydrin, sucrose synthase, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Expressed sequence tags; Transcription; Rootstock; Drought; Molecular response.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500019
Imagem não selecionada

Imprime registro no formato completo
In silico prediction of gene expression patterns in Citrus flavedo Genet. Mol. Biol.
Berger,Irving J.; Freitas-Astúa,Juliana; Reis,Marcelo S.; Targon,Maria Luísa P.N.; Machado,Marcos A..
Out of the 18,942 flavedo expressed sequences (clusters plus singletons) in Citrus sinensis from the Citrus EST Project (CitEST), 25 were statistically supported to be differentially expressed in this tissue after a double in silico hybridization strategy against leaf-, flower-, and bark-derived ESTs. Five of them, two terpene synthases and three O-methyltransferases, are absent in the other citrus tissues with concomitant 2x2 statistics, supporting the hypothesis that they are putative flavedo-specific expressed sequences. The pattern of these differentially expressed sequences during fruit development suggests that most of them are developmentally regulated. Some expressed gene products, including a putative germin-like protein highly expressed in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: EST; Fruit; Gene expression; Orange; Tissue-specific.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500004
Imagem não selecionada

Imprime registro no formato completo
Mapeamento de QTLs associados à produção de frutos e sementes em híbridos de Citrus Sunki vs. Poncirus trifoliata Rev. Bras. Frutic.
Siviero,Amauri; Cristofani,Mariângela; Boava,Leonardo P.; Machado,Marcos A..
O grupo dos citros apresenta acentuada juvenilidade manifestada pela incapacidade de florescimento, vigor e formação de espinhos. No caso da laranja 'valência', os híbridos de trifoliata, utilizados como porta-enxertos, induzem maior precocidade e produtividade. O número de sementes do fruto (NSF) constitui importante característica da variedade candidata a porta-enxerto de citros. O presente estudo teve como objetivo detectar QTLs associados às características número de frutos por planta (NF) e número de sementes por fruto em uma progênie F1 derivada de um cruzamento entre Citrus sunki e Poncirus trifoliata 'Rubidoux'. Os grupos de ligação (GL) dos parentais foram obtidos usando a estratégia 'pseudotestcross' e marcadores do tipo RAPD. A contagem do...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Propagação; Melhoramento de citros; Marcadores moleculares; Frutificação.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452002000300045
Imagem não selecionada

Imprime registro no formato completo
Polyclonal antibodies to the coat protein of Apple stem grooving virus expressed in Escherichia coli: production and use in immunodiagnosis Trop. Plant Pathol.
Nickel,Osmar; Targon,Maria L.P.N.; Fajardo,Thor V.M.; Machado,Marcos A.; Trivilin,Ana P..
The coat protein gene of Apple stem grooving virus (ASGV) was amplified by RT-PCR, cloned, sequenced and subcloned in the expression vector pMal-c2. This plasmid was used to transform Escherichia coli BL21c+ competent cells. The ASGV coat protein (cp) was expressed as a fusion protein containing a fragment of E. coli maltose binding protein (MBP). Bacterial cells were disrupted by sonication and the ASGVcp/MBP fusion protein was purified by amylose resin affinity chromatography. Polyclonal antibodies from rabbits immunized with the fusion protein gave specific reactions to ASGV from infected apple (Malus domestica) cv. Fuji Irradiada and Chenopodium quinoa at dilutions of up to 1:1,000 and 1:2,000, respectively, in plate trapped ELISA. The ASGVcp/MBP...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Recombinant antigen; Latent virus; ELISA; Malus domestica.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000500017
Imagem não selecionada

Imprime registro no formato completo
PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approach Genet. Mol. Biol.
Campos,Magnólia A.; Rosa,Daniel D.; Teixeira,Juliana Érika C.; Targon,Maria Luisa P.N.; Souza,Alessandra A.; Paiva,Luciano V.; Stach-Machado,Dagmar R.; Machado,Marcos A..
In silico expression profiles, of the discovered 3,103 citrus ESTs putatively encoding for PR protein families (PR-1 to PR-17), were evaluated using the Brazil citrus genome EST CitEST/database. Hierarchical clustering was displayed to identify similarities in expression patterns among citrus PR-like gene families (PRlgf) in 33 selected cDNA libraries. In this way, PRlgf preferentially expressed by organ and citrus species, and library conditions were highlighted. Changes in expression profiles of clusters for each of the 17 PRlgf expressed in organs infected by pathogens or drought-stressed citrus species were displayed for relative suppression or induction gene expression in relation to the counterpart control. Overall, few PRlgf showed expression 2-fold...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus; Functional genome; Ests; Gene expression profiles; Pathogenesis-related proteins; Defense genes.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500020
Imagem não selecionada

Imprime registro no formato completo
RT-PCR para a detecção simultânea dos vírus da leprose e da tristeza dos citros Trop. Plant Pathol.
Freitas-Astúa,Juliana; Locali,Eliane C.; Antonioli-Luizon,Renata; Astúa-Monge,Gustavo; Targon,Maria Luisa P.N.; Rodrigues,Vandeclei; Kitajima,Elliot W.; Machado,Marcos A..
Tipo: Info:eu-repo/semantics/report
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000600019
Imagem não selecionada

Imprime registro no formato completo
The induction of differentially expressed proteins of Xylella fastidiosa with citrus extract BJM
Bellato,Cláudia de M.; Garcia,Andréia K.M.; Mestrinelli,Fabiana; Tsai,Siu M.; Machado,Marcos A.; Meinhardt,Lyndel W..
An in vitro system was developed to induce and identify Xylella fastidiosa proteins that were differentially expressed in the presence of callus-derived extracts from its host, the citrus cultivar Pêra. To optimize the induction, we first developed a single culture medium for the growth of both, host and bacteria. This medium, CPXPm7, which mimics the citrus xylem sap, showed that X. fastidiosa at 72 h post-incubation had 10(8) colony forming units mL-1, while Pêra cells had the highest fresh weight content (0.79 g). After testing various methods of co-cultivation of the bacteria and host callus grown in this single medium, the best induction procedure was to grow X. fastidiosa in a solid medium amended with an extract of Pêra callus grown in CPXPm7....
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2DE; Callus; Pathogenicity; Protein; Xylella-citrus interaction.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200012
Imagem não selecionada

Imprime registro no formato completo
Transformation of Xanthomonas axonopodis pv. citri by electroporation Trop. Plant Pathol.
Amaral,Alexandre M. do; Toledo,Cristiane P.; Baptista,Juliana C.; Machado,Marcos A..
This study describes the use of electroporation for transforming Xanthomonas axonopodis pv. citri (Xac), the causal agent of citrus (Citrus spp.) canker. It also evaluates the methodology used for this species under different electrical parameters. The bacterium used in the study (Xac 306) was the same strain used for recent complete sequencing of the organism. The use of a plasmid (pUFR047, gentamycin r) is reported here to be able to replicate in cells of Xac. Following the preparation and resuspension of competent cells of Xac at a density of ~4 x 10(10) cfu/ml, in 10% glycerol, and the addition of the replicative plasmid, an electrical pulse was applied to each treatment. Selection of transformants showed a high efficiency of transformation (1.1 x...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus canker; Mutant; Citrus spp.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000300013
Registros recuperados: 18
Primeira ... 1 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional