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Correlation between chronic inflammation, immunostaining and parasite load in the genital system of female dogs naturally infected with Leishmania infantum Ciência Rural
Oliveira,Vinícius Vasconcelos Gomes de; Macedo,Simone Regina Barros de; Araújo,Flábio Ribeiro de; Ramos,Carlos Alberto do Nascimento; Ramos,Rafael Antonio Nascimento; Maia,Frederico Celso Lyra; Alves,Leucio Câmara; Silva Junior,Valdemiro Amaro da.
ABSTRACT: The aim of the present study was to investigate the correlations among chronic inflammatory reaction, immunostaining and parasite load in the genital system of female dogs naturally infected with Leishmania infantum . Animals (n = 10) used in this study were from the Department of Vector Control and Animal Surveillance of the municipality of Caruaru, state of Pernambuco, Brazil. Fragments of the vulva, vagina, cervix, uterine body, uterine horns and ovaries were submitted to histopathological analysis, immunohistochemistry (IHC) and DNA detection of amastigotes by qPCR. Correlations were found between the IHC findings and chronic inflammatory infiltrate related to L. Infantum only in the vulva and vagina; whereas, the same inflammatory reactions...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine visceral leishmaniasis; Genital pathology; Immunohistochemistry; QPCR.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001102029
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Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantum Ciência Rural
Oliveira,Vinícius Vasconcelos Gomes de; Macedo,Simone Regina Barros de; Araújo,Flábio Ribeiro de; Ramos,Carlos Alberto do Nascimento; Ramos,Rafael Antonio Nascimento; Maia,Frederico Celso Lyra; Alves,Leucio Câmara; Silva Junior,Valdemiro Amaro da.
ABSTRACT: Canine visceral leishmaniasisis an important disease caused by the protozoon Leishmania infantum which affects several organs and systems, including the genital tract. The L. infantum tropism to the male genital system and correlation among parasite load, immunohistochemistry detection and structural changes in these organs is controversy. Therefore, the aim of this study was to evaluate this correlation in the genital organs of the male dogs naturally infected with L. infantum. Samples from testicles, epididymis, prostate, glans penis, prepuce and scrotum were collected from 19 positive adult dogs. Structural changes were observed in the testicles (5.2%), epididymis (2.6%), prepuce (5.2%) and scrotum (5.2%) of the samples positive at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine visceral leishmaniasis; Structural changes; Genital pathology; Immunohistochemistry.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400641
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Histopathological findings and detection of parasites in the eyes of dogs infected naturally with Leishmania chagasi Ciência Rural
Brito,Fábio Luiz da Cunha; Laus,José Luiz; Tafuri,Wagner Luiz; Figueiredo,Maria Marta; Silva Júnior,Valdemiro Amaro; Maia,Frederico Celso Lyra; Alves,Leucio Câmara.
The objective of the present study was to investigate the histopathological alterations and detection of parasites that occur in the eyes of dogs naturally infected with Leishmania chagasi. The eyes of 25 dogs with a positive parasitological and serological exam for L. chagasi were submitted to routine ophthalmic examination. Fragments were obtained from the eyeball and were analyzed by immunohistochemistry and histopathology. Among the structures studied, the conjunctiva of the third eyelid was the tissue most frequently stained for L. chagasi. Histopathological analysis revealed a predominantly mononuclear inflammatory infiltrate whose intensity ranged from discrete to intense in the various ocular structures. Vascular congestion and perivasculitis were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine; Leishmaniasis; Ocular abnormalities; Histopathology; Immunohistochemistry.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000500022
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Mielografia de cães sadios com o meio de contraste ioversol: resultados liquóricos e anatomo-histopatológicos Ciência Rural
Sarmento,Luciana Virgínia Costa; Tudury,Eduardo Alberto; Teixeira,Miriam Nogueira; Maia,Frederico Celso Lyra; Albuquerque,Éricka Rejane Correia; Magalhães,Patrícia Karla de Luna.
O meio de contraste ioversol 240mg de iodo(I) por ml foi administrado na dose de 0,5ml/kg de peso por via intracisternal em 30 cães sadios, com o objetivo de verificar as possíveis alterações liquóricas e histopatológicas geradas pelo mesmo. Foram realizadas no líquor, análises físicas, contagens total e diferencial de leucócitos e determinação da concentração total de proteínas. A análise do líquor pós-mielográfica revelou aumento no número de leucócitos totais em três animais e da concentração de proteína em quatro. Alterações histopatológicas foram observadas em dois deles: infiltrado de células inflamatórias mononucleares na fissura ventral e no canal central da medula espinhal e infiltrado discreto de células inflamatórias mononucleares no canal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diagnóstico neurológico; Coluna espinhal; Cães.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000300010
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Molecular evidence of early vertical transmission of Leishmania ( Leishmania ) infantum in a dog Ciência Rural
Oliveira,Vinícius Vasconcelos Gomes de; Ramos,Rafael Antonio Nascimento; Ramos,Carlos Alberto do Nascimento; Guerra,Neurisvan Ramos; Maia,Frederico Celso Lyra; Alves,Leucio Câmara; Silva Junior,Valdemiro Amaro da.
ABSTRACT: Visceral leishmaniasis (VL) is caused by the protozoon Leishmania infantum . Transmission of this parasite to hosts occurs mainly through the bite of infected sand flies. However, alternative infection routes have been hypothesized, especially in areas where the biological vector is absent. The exact time of infection and whether in utero transmission occurs have still not been fully elucidated. This report demonstrates molecular evidence of vertical transmission of L. infantum from a pregnant dog to the embryo. Samples (e.g. vulva, vagina, cervix, uterine body, uterine horn and ovaries) from a female naturally infected by L. infantum and from her embryo were molecularly analyzed by means of qPCR and cPCR followed by DNA sequencing. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmaniasis; Dog; Vertical infection; Embryo.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100553
Registros recuperados: 5
Primeira ... 1 ... Última
 

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